Effects of Diatomaceous Earth on House Dust Mite Sensitization in BALB/c Mice

Abstract

Background: House Dust Mite (HDM) is associated with hypersensitivity such as asthma. Patients with asthma ben­efit from improved living environment by reducing HDM exposure. In this study, we examined the effects of com­mercialized diatomaceous earth product, Casaggia® used as construction materials, on hypersensitivity in HDM-sensitized mice.
 Methods: Male mice were sensitized with house dust mite extract for 7d and then housed in diatomaceous earth (DE)-coated cages for 14 days at Animal Center of the Taichung Veterans General Hospital, Taichung, Taiwan in 2014. Levels of cytokine were determined using ELISA. White blood cell counts were recorded over 21d. Histologi­cal anal­ysis was conducted to determine the remodeling of respiratory tract.
 Results: Exposure to DE resulted in a suppression in elevated eosinophilia induced by HDM in mice. In addition, elevated serum IgE responding to HDM sensitization were restored in the presence of DE. DE ameliorated the in­flammation progression in airway.
 Conclusion: Environmental exposure to DE is suggested to benefit patients with hypersensitivity through relieving inflammatory symptoms. In a sense of prevention, DE represents a potential material against development of asthma.