Effects of Diazoxide and Hydrochlorothiazide on Water Permeability and Sodium Transport in the Frog Bladder

Marumo, Fumiaki; Mishina, Takao; Shimada, Hajime

doi:10.1159/000137593

Cited by 9 publications

(8 citation statements)

References 5 publications

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“…Addition to both solutions caused a biphasic response. Marumo et al (36) examined the effect of HCTZ in the frog bladder and found partial inhibition of ISC after mucosal addition and no change in electrical properties after serosal addition. Thus, effects of thiazides on epithelia which have electrogenic Na absorption are not clearly understood.…”

Section: Discussionmentioning

confidence: 99%

Sodium chloride absorption by the urinary bladder of the winter flounder. A thiazide-sensitive, electrically neutral transport system.

Stokes¹

1984

J. Clin. Invest.

136

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Abstract. The urinary bladder of the winter flounder absorbs NaCl by a process independent of the transepithelial voltage. In contrast to most other epithelia which have a neutral NaCl-absorptive system, the flounder bladder has a high transepithelial resistance. This feature simplifies analysis of the cellular transport system because the rate of ion transfer through the paracellular pathway is rather low. Experiments were designed to distinguish among three possible mechanisms of neutral NaCl absorption: (a) Na/K/2Cl cotransport; (b) parallel Na/H and Cl/OH exchange; (c) and simple NaCl cotransport. A clear interdependency of Na and Cl for net absorption was demonstrated. NaCl absorption was not dependent on mucosal K and was minimally sensitive to loop diuretics. Thus a Na/K/2Cl transport system was unlikely. The mechanism was not parallel exchange as evidenced by insensitivity to amiloride and to 4,4'-diisothiocyano-2,2'-disulfonic stilbene, an inhibitor of anion exchange. In addition, inhibitors ofcarbonic anhydrase had no effect. Net absorption was almost completely abolished by hydrochlorothiazide (0.1 mM). Its action was rapid, reversible, and effective only from the mucosal surface. Metolazone, a structurally dissimilar diuretic in the benzothiadiazide class had qualitatively similar actions. The Dr. Stokes is an Established Investigator of the American Heart Association.

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Section: Discussionmentioning

confidence: 99%

Sodium chloride absorption by the urinary bladder of the winter flounder. A thiazide-sensitive, electrically neutral transport system.

Stokes¹

1984

J. Clin. Invest.

136

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“…In frog bladder, mucosal hydrochlorothiazide (5 x 1O-4 molll) quickly and reversibly inhibited the short circuit current whereas serosal application of the drug had no effect (MARUMO et al 1982). No effect could be observed on ADH-stimulated water transport by the bladder.…”

Section: Other Mechanismsmentioning

confidence: 95%

“…In some systems, thiazides inhibit electrogenic Na+ transport (PENDLETON et al 1968;SCHNIEDERS and LUDENS 1980;SULLIVAN et al 1971;MARUMO et al 1982;ERIKSSON and WISTRAND 1987). In the isolated toad (Bufo marinus) bladder preparation, bendroflumethiazide (1.6 x 10-4 mmol/l) when added to the serosal side of the bladder decreased the short circuit current, active Na+ flux, and trans epithelial voltage and conductance (PENDLETON et al 1968).…”

Section: Other Mechanismsmentioning

confidence: 99%

Diuretics

1995

Handbook of Experimental Pharmacology

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“…Each hemibladdcr was mounted in a glass chamber. To prevent mechanical distortion of the bladder membrane, both orifices of the chambers were covered by nylon mesh as previously described [7], To measure the osmotic water flow volumetrically. Ringer's solution diluted by 1:5 was introduced into the mucosal chamber.…”

Section: Methodsmentioning

confidence: 99%

Effects of ONO-3122 (an Enhancer of PGH<sub>2</sub> Production) and OKY-1581 (an Inhibitor of TXA<sub>2</sub> Production) on the Vasopressin-Induced Water Flow in the Toad Bladder

Marumo¹

1985

Pharmacology

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The effects of 1-iodo-3-aminomethyl-5,6,7,8-tetrahydro-2-naphthol (ONO-3122) which increases endogenous PGH₂, and sodium (E)-2-methyl-3-[4-3-pyridylmethyl)phenyl]-2-methylpropenoate (OKY-1581) which inhibits thromboxane A₂ synthesis, on vasopressin-induced osmotic water flow in the bladder of the toad, Bufo bufo japonicus, were examined. ONO-3122 significantly inhibited the vasopressin-induced water flow at a concentration of 1 × 10^–4M. OKY-1581 inhibited the vasopressin-induced water flow at 1 × 10^–6 M, but enhanced it at 1 × 10^–4M. These results suggest that ONO-3122 indirectly inhibits the vasopressin-induced osmotic water flow in the toad bladder; that is, ONO-3122 causes an increase in the conversion of arachidonic acid into PGH₂. These results also suggest that OKY-1581 at a low concentration suppresses the vasopressin-induced water flow due to inhibition of cyclooxygenase activity. Both ONO-3122 and OKY-1581 provide a useful means for studying the action of prostaglandins.

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Effects of Diazoxide and Hydrochlorothiazide on Water Permeability and Sodium Transport in the Frog Bladder

Cited by 9 publications

References 5 publications

Sodium chloride absorption by the urinary bladder of the winter flounder. A thiazide-sensitive, electrically neutral transport system.

Sodium chloride absorption by the urinary bladder of the winter flounder. A thiazide-sensitive, electrically neutral transport system.

Diuretics

Effects of ONO-3122 (an Enhancer of PGH<sub>2</sub> Production) and OKY-1581 (an Inhibitor of TXA<sub>2</sub> Production) on the Vasopressin-Induced Water Flow in the Toad Bladder

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