Effects of diet and cold exposure on rates of plasma leucine turnover and protein synthesis in sheep

An isotope dilution method of [2H5]phenylalanine (Phe) and [2H2]tyrosine (Tyr) was used to determine the effects of metabolisable energy (ME) intake and cold exposure on plasma Phe and Tyr turnover rates in sheep. Whole body protein synthesis (WBPS) was calculated with the [2H5]Phe model. Eight adult sheep were assigned to two dietary treatments receiving the same amount of crude protein and either 515 or 828 kJ x kg BW(-0.75) x d(-1) of ME (Me-ME diet and Hi-ME diet, respectively) with a crossover design for two 28 d periods. The sheep were exposed from a thermoneutral environment (23 +/- 1 degrees C) to a cold environment (2 +/- 1 to 4 +/- 1 degrees C) for 6 d for each dietary treatment. The primed-continuous infusion method of isotope dilution was conducted in both environmental temperatures. Plasma Phe turnover rate (PheTR) tended to be greater and plasma Tyr turnover rate (TyrTR) was greater (p = 0.03) for the Hi-ME diet compared with the Me-ME diet. Plasma PheTR increased (p = 0.04) and plasma TyrTR tended to increase during cold exposure. Whole body protein synthesis tended to be greater for the Hi-ME diet compared with the Me-ME diet and increased (p = 0.03) during cold exposure compared to the thermoneutral environment, but no interaction was detected. It was concluded that in sheep, plasma PheTR and WBPS (as determined by the [2H5]Phe model) tended to be influenced by and plasma TyrTR was influenced by ME intake. Further, plasma PheTR and WBPS increased and plasma TyrTR tended to increase during cold exposure.

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Intermediary Metabolism of Plasma Acetic Acid, Glucose and Protein in Sheep Fed a Rice Straw-based Diet

Alam¹,

Ogata²,

Sako³

et al. 2010

Asian Australas. J. Anim. Sci

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The present study was conducted to determine plasma acetate, glucose and protein metabolism using dilution of isotopes [[1-13 C]Na acetate, [U-13 C]glucose and [1-13 C]leucine (Leu)] in sheep fed rice straw (Oriza japonica L.). Four sheep were assigned to either rice straw (RS-diet) or mixed hay (MH-diet) with a crossover design. Nitrogen (N) intake and N digestibility were lower (p = 0.002 and p = 0.02, respectively) for RS-diet than MH-diet, but N retention did not differ (p>0.10) between the diets. Concentrations of rumen acetate tended to be lower (p = 0.07), and propionate was higher (p = 0.02) for RS-diet than MH-diet. Concentrations of plasma lactate, non-esterified fatty acids, Leu and α-ketoisocaproic acid did not differ (p>0.10) between the diets, but plasma glucose and urea concentrations were lower (p = 0.01 and p = 0.003, respectively) for RS-diet than MH-diet. Turnover rate of plasma acetate did not differ (p = 0.39) between the diets, and plasma glucose and Leu turnover rates were numerically lower (p = 0.15 and p = 0.14, respectively) for RS-diet than MH-diet. Whole body protein synthesis and degradation did not differ (p>0.10) between the diets. Thus it can be concluded that the intermediary metabolism of acetate, glucose and protein on rice straw is comparable to mixed hay in sheep.

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Effects of diet and cold exposure on rates of plasma leucine turnover and protein synthesis in sheep

Cited by 3 publications

References 27 publications

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Effects of dietary energy intake and cold exposure on kinetics of plasma phenylalanine, tyrosine and protein synthesis in sheep

Intermediary Metabolism of Plasma Acetic Acid, Glucose and Protein in Sheep Fed a Rice Straw-based Diet

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