Effects of dietary arginine levels and carbohydrate-to-lipid ratios on mRNA expression of growth-related hormones in largemouth bass, Micropterus salmoides

Chen, Naisong; Jin, Lina; HengYong, Zhou; Qiu, Xiaojie

doi:10.1016/j.ygcen.2012.08.004

Cited by 44 publications

(19 citation statements)

References 35 publications

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“…) Chen et al (2012). reported that igf-I expression level in liver of M. salmoides decreased with the increase of dietary carbohydrate, but the igf-I content in serum was not affected by dietary carbohydrate in this study.…”

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confidence: 48%

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Transcriptome, enzyme activity and histopathology analysis reveal the effects of high level of dietary carbohydrate on glycometabolism in juvenile golden pompano,Trachinotus ovatus

Liu

Tan

et al. 2019

Aquac Res

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Golden pompano (Trachinotus ovatus) is an important aquaculture species in Chinaand broadly cultivated in Asia-Pacific region. Our previous study found that T. ovatus had a better tolerance to the high level of carbohydrate in diet (360 g/kg) compared to Micropterus salmoides. Present study investigated the tolerance mechanism of T. ovatus to high level of dietary carbohydrate (PH-360 g/kg of corn starch) on transcriptome level using Illumina sequencing compared to optimum level of dietary carbohydrate (PM-240 g/kg corn starch). A total of 208,952,492 reads were obtained and assembled into 62,377 unigenes with an average length of 822 bp and a range of 200-16,206 bp. Transcriptome annotation revealed that 27,636 unigenes with a cut-off e-value of 10 −5 were functionally annotated. Specifically, 7,903 unigenes were clustered into 24 functional categories by searching against the COG database, 12,161 unigenes to 84 GO terms, 26,835 unigenes to Nr database and 23,681 unigenes to SWISS-PROT database. In addition, 13,739 unigenes were grouped into 240 KEGG pathways. There are 3,461 differentially expressed genes in PH compared to PM, in which 3,059 unigenes were significantly upregulated and 402 unigenes were significantly downregulated. Twelve key genes related to carbohydrate metabolism were further validated by quantitative real-time RT-PCR, and the results were consistent with those of transcriptome profile. Under high level of dietary carbohydrate (PH-360 g/kg), the results indicate that T. ovatus can adapt to the high level of carbohydrate in diet by enhancing glycolysis with hepatic HK enzyme activity was significantly increased (p > 0.05), reducing gluconeogenesis with the decreased trend of PEPCK enzyme activity and maintaining the homeostasis of carbohydrate metabolism-related hormones and hormone receptor gene expressions. Histopathological examination has shown that liver cavity area was significantly increased (p > 0.05), but no visible nutritional diseases are found in fish. This study provides a valuable understanding of the molecular responses of T. ovatus fed by the high level of dietary carbohydrate (PH-360 g/kg).

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confidence: 48%

“…Among the hormones, glucagon, IGF-I and insulin are closely related to glucose metabolism (Chen, Jin, Zhou, & Qiu, 2012;Enes, Sanchez-Gurmaches, Navarro, Gutiérrez, & Oliva-Teles, 2010).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome, enzyme activity and histopathology analysis reveal the effects of high level of dietary carbohydrate on glycometabolism in juvenile golden pompano,Trachinotus ovatus

Liu

Tan

et al. 2019

Aquac Res

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“…The expression of LARS was up-regulated by dietary leucine in the hepatopancreas of gibel carp. Moreover, Chen, Jin, Zhou and Qiu (2012) reported that expression of the TOR signalling pathway genes might exist in different patterns in different tissues or organs. An in vitro study using primary cell culture also suggested that leucine was able to activate the Akt and TOR signalling pathway of rainbow trout hepatocytes (Lansard et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibeliovar. CAS III)

Zou

Cao

et al. 2018

Aquac Res

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An 8-week feeding trial was conducted to investigate the impacts of dietary leucine on growth, hepatopancreas and muscle protein content and relative expressions of protein synthesis-related genes in juvenile gibel carp (5.2 g). Triplicate tanks of fish (22 fish per tank) were fed with a low (0.82%), middle (1.90%) or high (2.99%) leucine diet to apparent satiation three times a day. The results showed that the weight gain (WG) and feed efficiency (FE) of the fish fed with the 1.90% leucine diet had the highest values compared to those of other two groups (p < .05). Dietary leucine significantly affected the protein and lipid contents of the hepatopancreas (p < .05), but not muscle or the whole body composition of the fish. The amino acid contents (Arginine, Leucine, Methionine, Phenylalanine, Alanine, Aspartic acid, Glutamic acid and Proline) of the dorsal muscles of fish fed with a 1.90% leucine diet were significantly higher than those fed with a 0.82% leucine diet. Moreover, transcriptional levels of target genes of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1), eukaryotic translation initiation factor 4E (eIF4E) and leucyl-tRNA synthetase (LARS) in the hepatopancreas were significantly up-regulated (p < .05), but eukaryotic translation initiation factor 4E-binding protein 2 (4E-BP2) was significantly down-regulated (p < .05) with the increasing dietary leucine levels. However, dietary leucine had no effects on the gene expressions of 4E-BP2 and S6K1 in the muscle of gibel carp. The present results indicate that dietary leucine led to a marked tissue-specific response on protein content in the muscle and hepatopancreas of gibel carp that is related to the TOR signalling pathway.

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“…Arginine is considered as a versatile amino acid in organisms due to several physiological roles (Wu et al, 2009). Arginine may induce somatotropic axis in fish characterized by increase in insulin (Plisetskaya et al, 1991; Baños et al, 1997; Sink & Lochmann, 2007; Andoh, 2014), growth hormone (GH) and insulin‐like growth factor 1 (IGF‐1) (Zhou et al, 2011; Chen et al, 2012b; Pohlenz et al, 2013; Wang et al, 2017), which seems to be related to the growth promoting effects of arginine. Some evidences suggested that dietary arginine may contribute to stress and cortisol responses in fish (Costas et al, 2008; Costas et al, 2011; Costas et al, 2013; Hoseini et al, 2019b).…”

Section: Introductionmentioning

confidence: 99%

Roles of arginine in fish nutrition and health: insights for future researches

Hoseini

Khan

Yousefi

et al. 2020

Reviews in Aquaculture

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Amino acids are important molecules in fish nutrition with critical roles in protein synthesis, immune function and health. Among the essential amino acids, arginine is one of the functional amino acids in animals. Arginine requirement in fish ranges between 3.0 and 8.1% of dietary protein. The amino acid involves in ureagenesis, immune function, antioxidant defence, somatotropic axis, stress responses and ammonia detoxification in fish. To have an extended insight about the current knowledge of arginine effects in fish, this article reviewed available data on arginine effects in different fish species. It covers variations in arginine requirements of different fish species, interaction of arginine with lysine and glutamine, modulation of ureagenesis and ammonia detoxification, modulation of insulin and growth hormone responses, cortisol response, modulation of antioxidant system, immune responses and disease resistance. This review indicates that rearing conditions and dietary ingredients may affect fish arginine requirement. In addition, there is a need for further data about the antioxidant effects of arginine under oxidative condition, its immunostimulant effects under pathogenic challenge, mechanisms of ammonia detoxification and cortisol response by arginine in different fish species.

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Effects of dietary arginine levels and carbohydrate-to-lipid ratios on mRNA expression of growth-related hormones in largemouth bass, Micropterus salmoides

Cited by 44 publications

References 35 publications

Transcriptome, enzyme activity and histopathology analysis reveal the effects of high level of dietary carbohydrate on glycometabolism in juvenile golden pompano,Trachinotus ovatus

Transcriptome, enzyme activity and histopathology analysis reveal the effects of high level of dietary carbohydrate on glycometabolism in juvenile golden pompano,Trachinotus ovatus

Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibeliovar. CAS III)

Roles of arginine in fish nutrition and health: insights for future researches

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