Effects of dietary Astragalus polysaccharides on growth, health and resistance to Vibrio harveyi of Lates calcarifer

Yu, Wei; Yang, Ye; Zhou, Qicun; Huang, Xiaolin; Huang, Zhong; Li, Tao; Wu, Qiaer; Zhou, Chuanpeng; Ma, Zhenhua; Lin, Heizhao

doi:10.1016/j.ijbiomac.2022.03.176

“…In the present study, 400 mg/kg AMP significantly promoted WGR, SGR, and FE. A similar growth promotion effect of polysaccharide additives was previously reported in tilapia [32], turbot [33], grey mullet [34], large yellow croaker [35], Asian seabass [36], and shrimp [37]. However, in this study, no significant difference in growth was found between fish with dietary AMP levels of 4000 mg/kg and 8000 mg/kg.…”

Section: Discussionsupporting

confidence: 90%

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Dong

¹

,

Wu

²

,

Chen

³

et al. 2022

View full text Add to dashboard Cite

Atractylodes macrocephala polysaccharide (AMP) can enhance antioxidant defense and anti-inflammation, as the tolerance levels of AMP in aquaculture is important for additive utilization. However, the tolerance dose of AMP is unknown. We assess the tolerance levels of AMP in juvenile largemouth bass (3.38 ± 0.11 g) by feeding them a 0, 400, 4000, or 8000 mg/kg AMP supplemented diet for 10 weeks. The 400 mg/kg AMP dose increased growth performance. The Nrf2/Keap1 signaling pathway was activated, as indicated by Keap1 and Nrf2 protein levels in the liver. Enhanced activity of antioxidant enzymes (SOD, GPx), together with increased mRNA levels of antioxidant genes (sod, gpx) and decreased accumulation of reactive oxygen species (ROS) and MDA, was found in the liver, implying the antioxidant effect of AMP. Nutrient absorption was enhanced by AMP, as reflected by the increased length of intestinal villi and microvilli. However, 4000 and 8000 mg/kg AMP induced oxidant stress, as indicated by increased plasma ALT and AST content and decreased mRNA levels of antioxidant genes (sod, gpx) in the liver and intestinal tissues. Inflammatory reactions were also induced by high doses of AMP, as reflected by enhanced levels of pro-inflammatory cytokines (tnfα, nfκb) in the liver, intestinal, and kidney tissues and inhibited levels of anti-inflammatory cytokines (tgfβ, iκb). Histological analysis reveals inflammatory cell infiltration and tissue damage. Thus, the safe tolerance margin of AMP supplement for largemouth bass was 400–4000 mg/kg.

show abstract

“…Immunoglobulins are essential indicators of the immune system and play an important role in the immune response ( Mikocziova, Greiff & Sollid, 2021 ). Studies have shown that plant polysaccharides could improve the immune function of poultry ( Long et al, 2021 ; Yu et al, 2022 ) and activate macrophages to exert immunomodulatory effects by recognizing and binding to specific receptors ( Zhang et al., 2021 ). In our study, several herbs of CHAA and CHAB are rich in polysaccharides, such as Codonopsis pilosula , Atractylodes and Poria cocos.…”

Section: Discussionmentioning

confidence: 99%

The effect of combined dietary supplementation of herbal additives on carcass traits, meat quality, immunity and cecal microbiota composition in Hungarian white geese

Fu

¹

,

Zhou

²

,

Song

³

et al. 2023

PeerJ

View full text Add to dashboard Cite

The present study was performed to investigate the effects of dietary supplementation with herbal additives on meat quality, slaughter performance and the cecal microbial community in Hungarian white geese. A total of 60 newborn geese were assigned equally into the control group (CON) and the herbal complex supplemented group (HS). The dietary supplementations consisted of Compound Herbal Additive A (CHAA) including Pulsatilla, Gentian and Rhizoma coptidis, and Compound Herbal Additive B (CHAB) containing Codonopsis pilosula, Atractylodes, Poria cocos and Licorice. The geese in the HS group received a basal diet supplemented with 0.2% CHAA from day 0 to day 42 at the postnatal stage. Then from day 43 to day 70, the geese in HS group were provide a basal diet with 0.15% CHAB. The geese in the CON group were only provided with the basal diet. The results showed that the slaughter rate (SR), half chamber rates (HCR), eviscerated rate (ER) and breast muscle rate (BMR) in the HS group tended to increase slightly compared with the CON group (ns). In addition, the shear force, filtration rate and pH value of breast muscle and thigh muscle in the HS group were slightly enhanced compared to the CON group (ns). Significant increased levels in carbohydrate content, fat content and energy (P < 0.01) and significant decreased levels in cholesterol content (P < 0.01) were observed in the muscle of the HS group. The total amino acid (Glu, Lys, Thr and Asp) content in the muscle increased in HS group than in the CON group (P < 0.01). Dietary herb supplementations significantly increased the levels of IgG in serum (P < 0.05) on day 43 and higher levels of IgM, IgA and IgG (P < 0.01) were also observed in the HS group on day 70. Furthermore, 16S rRNA sequencing results indicated that herbal additives increased the growth of beneficial bacteria and inhibited the proliferation of harmful bacteria in the geese caecum. Altogether, these results offer crucial insights into the potential benefits of incorporating CHAA and CHAB into the diets of Hungarian white goose. The findings indicate that such supplementations could significantly improve meat quality, regulate the immune system and shape the intestinal microbiota composition.

show abstract

“…Tis is consistent with the results of growth performance of larvae. Previous studies confrmed that feeding sodium alginate, astragalus, and linseed could improve the activities of digestive enzymes in Asian seabass (Lates calcarifer) and silver catfsh (Rhamdia quelen) [27][28][29]. Meanwhile, AKP and LAP were considered as the important indicator of digestive function, which is located in brush border membranes of fsh foregut epithelial cells [30,31].…”

Section: Discussionmentioning

confidence: 99%

Tragacanth Gum and Linseed Gum as Adhesives Improved the Survival, Digestive Function, Antioxidant Enzyme Activities, and Immunity in Large Yellow Croaker (Larimichthys crocea) Larvae

Liu

¹

,

Wang

²

,

Zhang

³

et al. 2023

Aquaculture Research

2

0

View full text Add to dashboard Cite

A 30-day feeding experiment was designed to investigate the effects of different adhesives on survival, growth performance, digestive enzyme activities, antioxidant capacity, and inflammation response of large yellow croaker (Larimichthys crocea) larvae (initial weight 8.40 ± 0.18 mg). Four iso-nitrogenous (55% crude protein) and iso-lipidic (22% crude lipid) microbound diets (MBD) were formulated to contain 2% sodium alginate (SA), 2% tragacanth gum (TG), 2% linseed gum (LG), and 2% poloxamer (PX), respectively. Results showed that larvae fed with TG and LG diets had significantly higher survival rate than the larvae fed with SA and PX ( P < 0.05 ). Larvae fed with the PX diet had lower final weight and specific growth rate than the other groups ( P < 0.05 ). Larvae fed with TG diet had significant higher activities of lipase in pancreatic segments and trypsin in intestinal segments than the larvae fed with the PX ( P < 0.05 ). Meanwhile, the lipase activities of larvae fed with LG was significantly higher in intestinal segments than those fed with SA and PX diets ( P < 0.05 ). Larvae fed with TG and LG diets had significantly higher activities of AKP in brush border membranes than larvae fed with SA and PX diets ( P < 0.05 ). Moreover, the mRNA expression of pcna and zo-1 increased, respectively, in larvae fed TG and LG diets compared with the other groups ( P < 0.05 ). Larvae fed with LG and TG diets had significantly higher activities of SOD and T-AOC than those fed with PX diet ( P < 0.05 ). The content of GSH increased significantly in larvae fed with SA, TG, and LG diets compared with PX treatment ( P < 0.05 ). The activities of iNOS significantly increased in larvae fed with TG and LG diets compared to other groups ( P < 0.05 ). Meanwhile, the mRNA expression of il-10 significantly increased in TG and LG groups ( P < 0.05 ). In conclusion, results of the study demonstrated that TG and LG can be used as feed binders for microdiets with positive effects on survival, activities of digestive enzymes, antioxidant capacity, and inflammatory response. However, PX inhibited the survival and growth, and further study is needed to determine the suitable usage and dosage.

show abstract

Effects of dietary Astragalus polysaccharides on growth, health and resistance to Vibrio harveyi of Lates calcarifer

Cited by 43 publications

References 39 publications

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

The effect of combined dietary supplementation of herbal additives on carcass traits, meat quality, immunity and cecal microbiota composition in Hungarian white geese

Tragacanth Gum and Linseed Gum as Adhesives Improved the Survival, Digestive Function, Antioxidant Enzyme Activities, and Immunity in Large Yellow Croaker (Larimichthys crocea) Larvae

Contact Info

Product

Resources

About