Effects of dietary daidzein supplementation on reproductive performance, immunity, and antioxidative capacity of New Zealand White does

Xie, Hongwei; Yu, En; Wen, Huamei; Jiang, Bayi; Fu, Guihua; Sun, Haitao; He, Jun

doi:10.1016/j.anifeedsci.2022.115431

Cited by 4 publications

(11 citation statements)

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“…The experimental diets were formulated to meet the nutritional needs of rabbits according to recommendations [30]. The ingredients and chemical compositions are shown in Table 1, and the chemical analysis was previously described [20]. We recorded the litter size, weighed the individual body weight (BW), and calculated the average daily gain (ADG) of neonatal rabbits during the trial.…”

Section: Animals Housing and Dietsmentioning

confidence: 99%

“…The neonatal rabbits were anaesthetized with anhydrous ether and blood samples were collected via the heart. All blood samples were processed as described previously [20]. After blood sampling, these neonatal does were sacrificed to collect the duodenal, jejunal, and ileal samples (the middle part of each intestinal segment was approximately 4 cm), fixed with 4% paraformaldehyde solution for intestinal morphology examination.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Moreover, the serum metabolites, such as high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglyceride (TG), were tested with a commercial kit (The Institute of Nanjing Jiancheng Bioengineering Institute, Nanjing, China). All procedures were referred to in previous studies [20].…”

Section: Serum Hormones and Metabolite Level Analysismentioning

confidence: 99%

“…Daidzein (DA), a kind of isoflavone, is one of the major phytoestrogens which is abundant in soybeans, pasture grasses, and whole cereals [20]. Previous studies indicated that dietary DA powder supplementation doses ranging from 50 to 200 mg/kg could promote growth, improve animal fertility, and ameliorate oxidative stress and inflammatory responses in rabbits, cows, and hens [20][21][22][23]. However, the DA supplementation of more than 400 mg/kg was observed to have a negative effect on body weight gain and splenic morphology in piglets [24].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, this is the first study to explore the influence of maternal DA supplementation on growth and intestinal development in neonatal rabbits. Our previous study explored the influence of DA on the reproductive performance, immunity, and antioxidative ability of maternal does [20], and we hypothesized that maternal DA supplementation would improve the poor growth performance of neonatal rabbits due to increased litter size and insufficient milk yield. Hence, the objective of this experiment was to explore the influence and possible optimal dose of maternal DA powder supplementation during lactation on growth performance, antioxidative capacity, and intestinal health in neonatal rabbits.…”

Section: Introductionmentioning

confidence: 99%

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Maternal Daidzein Supplementation during Lactation Promotes Growth Performance, Immunity, and Intestinal Health in Neonatal Rabbits

Xie,

Yu,

Wen

et al. 2023

Agriculture

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The main purpose of the present research was to evaluate the effect of varying levels of DA inclusion in maternal diet, in the form of powder, on the born-weaning growth performance (days 1–30) and intestinal health of neonatal rabbits. A total of 152 delivered maternal does (3.94 ± 0.05 kg) were allocated into four groups, with thirty-eight replicates of one doe each, and fed with a control diet (CON) supplemented with different levels of powdered DA (85 mg/kg (DA85), 170 mg/kg (DA170), and 340 mg/kg (DA340)) during lactation. The results show that dietary DA increased individual body weight at days 21 and 30 (p = 0.03 and p < 0.01) and showed a linear and quadratic effect on individual body weight and average daily gain (ADG) (p < 0.05), although ADG was not affected by DA. The serum progesterone (P) (p = 0.05) and glutathione peroxidase (GSH-Px) (p = 0.01) concentrations of maternal rabbits were increased in all maternal DA-supplemented groups and showed a linear and quadratic effect (p < 0.05), while a linear effect was observed in estrogen (E-2) (p < 0.05). Interestingly, the concentrations of the serum insulin-like growth factor-1 (IGF-I) (p < 0.01) and immunoglobulin (IgA and IgG) (p < 0.01 and p = 0.01) in neonatal rabbits were increased in all dietary DA groups, and both showed a linear and quadratic effect (p < 0.05). In addition, the serum interleukin (IL-1β) (p = 0.03) and catalase (CAT) (p = 0.04) concentrations were affected by DA supplements, but linear and quadratic effects were only observed in the catalase (CAT) of neonatal rabbits (p < 0.05). Importantly, the duodenal and jejunal villus heights (p < 0.04 and p = 0.01) and the ratio of villus height to crypt depth (p = 0.02 and p = 0.01) in neonatal rabbits were elevated in all DA-supplemented treatments, while a linear and quadratic effect was observed in jejunum, but a quadratic effect was observed in duodenum (p < 0.05). The ileal villus height (p < 0.01) was also elevated in all DA-supplemented treatments exhibiting both linear and quadratic effects (p < 0.05). Moreover, the expression levels of occludin (p = 0.04), zonula occludens-1 (ZO-1) (p < 0.01), IGF-1 (p = 0.03), and solute carrier family 5 member 9 (SCL5A9) (p < 0.01) in jejunum were markedly elevated in the DA175 and 340 mg/kg DA-supplemented groups and were showing linear and quadratic effects (p < 0.05). Finally, inflammation-related gene expression levels such as NF-κB, TLR4, and MYD88 in jejunal (p = 0.01, p = 0.04, and p < 0.01) and ileal (p = 0.04, p = 0.04, and p = 0.03) epithelium were decreased in the DA170 and DA340 groups (p < 0.05), while linear and quadratic effects were observed for TLR4 in jejunum and for NF-κB and TLR4 MYD88 in ileum (p < 0.05). In summary, as a functional additive, maternal DA supplementation with 170 and 340 mg/kg DA during lactation can promote the growth of neonatal rabbits, which is related to improved antioxidative capacity and immunity, as well as improved intestinal health in neonatal rabbits.

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Section: Animals Housing and Dietsmentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

Section: Serum Hormones and Metabolite Level Analysismentioning

confidence: 99%