Effects of dietary docosahexaenoic acid (22:6n-3) and arachidonic acid (20:4n-6) on the growth, survival, stress resistance and fatty acid composition in black sea bass Centropristis striata (Linnaeus 1758) larvae

Abstract

The objectives of this study were to determine the effects of the dietary docosahexaenoic acid (DHA) to arachidonic acid (ARA) ratio on the survival, growth, hypersaline stress resistance and tissue composition of black sea bass larvae raised from ¢rst feeding to metamorphic stages. Larvae were fed enriched rotifers Brachionus rotundiformis and Artemia nauplii containing two levels of DHA (0% and10% total fatty acids 5 TFA) in conjunction with three levels of ARA (0%, 3% and 6% TFA). On d24ph, larvae fed the 10:6 (DHA:ARA) treatment showed signi¢cantly (Po0.05) higher survival (62.3%) than larvae fed 0:0 (DHA:ARA) (27.4%). Notochord length and dry weight were also signi¢cantly (Po0.05) greater in the 10:6 (DHA:ARA) treatment (8.65 mm, 2.14 mg) than in the 0:0 (DHA:ARA) (7.7 mm, 1.65 mg) treatment. During hypersaline (65 g L À 1 ) challenge, no signi¢cant di¡erences (P40.05) were observed in the median survival time (ST50) between larvae fed 10% DHA (ST50 525.6 min) and larvae fed 0% DHA (ST50 518.2 min). The results suggested that black sea bass larvae fed prey containing 10% DHAwith increasing ARA within the range of 0^6% showed improved growth and survival from ¢rst feeding through metamorphic stages.

Effects of dietary soybean oil inclusion to replace fish oil on growth, muscle fatty acid composition, and immune responses of juvenile darkbarbel catfish, Pelteobagrus vachelli

“…Our result indicated that inclusion of soybean oil in feed does not compromise the species growth, suggesting the possible replacement of fish oil with soybean oil in diet formulation for darkbarbel catfish. The positive relationship between tissue fatty acid composition and dietary fatty acid contents has been well demonstrated in previous studies (Bransden et al, 2005;Zia-Ul-haq et al, 2007a, b, 2008, 2011aLuo et al, 2008;Rezek et al, 2010). In this study, the muscle fatty acid composition in fish generally reflected the lipid composition in diets similar to the report of Ng et al (2003) that the high fatty acids in feed can lead to high fatty acids in muscle.…”

Molecular cloning and expression analysis of the retinoid X receptor (RXR) gene in golden pompano Trachinotus ovatus fed Artemia nauplii with different enrichments

“…However, low survival in the Algamac 3080 treatment may also contribute to the high SGR due to the death of small larvae in this treatment. Highly unsaturated fatty acids, especially EPA, DHA, and ARA, are essential to growth, development, and survival in marine fish (Sargent et al 1999b;Cahu et al 2003a;Rezek et al 2010). To develop lipidenriched food for fish larvae, the requirements of essential fatty acids in fish larvae have been extensively studied by using live prey enriched with different oils and micronutrients, aiming to increase the essential fatty acids content in live prey (Sargent et al 1997;Takeuchi 1997;Izquierdo et al 2000).…”

“…In marine fish larvae, both DHA and EPA in the diet are essential to fish growth (Rezek et al 2010). Improvement in fish growth with increasing levels of dietary DHA has been observed in species such as yellowtail Seiola quiqueradiata (Furuita et al 1996), striped jack Caranx vinctus , and Japanese flounder Paralichthys olivaceus (Izquierdo et al 1992).…”

Effect of feeding Artemia nauplii enriched with different enhancement products on larval performance of golden pompano Trachinotus ovatus (Linnaeus, 1758)

“…In marine fish larvae both DHA and EPA in the diet are essential for fish growth (Rezek et al, 2010). Improved fish growth with increasing levels of dietary DHA has been observed in species such as yellowtail, Seiola quiqueradiata (Furuita et al, 1996), striped jack Caranx vinctus and Japanese flounder Paralichthys olivaceus (Izquierdo et al, 1992).…”

“…Highly unsaturated fatty acids, especially EPA, DHA and ARA are essential for growth, development and survival in marine fish (Sargent et al, 1999;Cahu et al, 2003;Rezek et al, 2010). To develop lipid enriched food for fish larvae, the requirements of essential fatty acids in fish larvae have been extensively studied using live prey enriched with different oils and micro-nutrients, aiming to increase the essential fatty acids content in live prey Takeuchi, 1997;Izquierdo et al, 2000).…”