Effects of dietary fat and oxidized cholesterol on gene expression in rat liver as assessed by cDNA expression array analysis

Ringseis, Robert; Eder, Klaus

doi:10.1007/s00394-004-0515-x

Cited by 9 publications

(7 citation statements)

References 40 publications

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“…Dietary PUFA greatly influence serum lipid concentrations, lipid profiles, and lipid metabolism by regulating gene expression through alteration of transcription factors involved in absorption, extracellular transport, cellular uptake, metabolism, and elimination of lipids in the animal ( Jump, 2002 ; Ringseis and Eder, 2005 ). Dietary supplementation with oxidized oil resulted in higher levels of protein carbonyl formation in blood serum ( Figure 1 ), in agreement with Zhang et al (2011b) , who reported that 5% inclusion of oxidized animal-vegetable fat in broiler diets increased plasma carbonyl content.…”

Section: Resultsmentioning

confidence: 99%

Dietary antioxidant supplementation enhances lipid and protein oxidative stability of chicken broiler meat through promotion of antioxidant enzyme activity

et al. 2014

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Recent nutrigenomic studies have shown that animal nutrition can have a major influence on tissue gene expression. Dietary antioxidant supplements can enhance the quality of meat through modification of tissue metabolic processes. This study investigated the influence of dietary antioxidants and quality of oil on the oxidative and enzymatic properties of chicken broiler breast meat stored in an oxygen-enriched package (HiOx: 80% O2/20% CO2) in comparison with air-permeable polyvinylchloride (PVC) or skin packaging systems during retail display at 2 to 4°C for up to 21 d. Broilers were fed either a diet with a low-oxidized (peroxide value 23 mEq of O2/kg) or high-oxidized (peroxide value 121 mEq of O2/kg) oil, supplemented with or without an algae-based Se yeast and organic mineral antioxidant pack for 42 d. Lipid and protein oxidation and tissue enzymatic activity were analyzed. In all packaging systems, lipid oxidation (TBA reactive substances) was inhibited by up to 32.5% (P < 0.05) with an antioxidant-supplemented diet when compared with diets without antioxidants, particularly in the HiOx and PVC systems. Protein sulfhydryls were significantly protected by antioxidant diets (e.g., by 14.6 and 17.8% for low-and high-oxidized dietary groups, respectively, in PVC d 7 samples). Glutathione peroxidase, catalase, and superoxide dismutase activities were significantly higher (P < 0.05) in antioxidant-supplemented diets compared with the basal diet, regardless of oil quality. Also, serum carbonyls were lower in broilers fed a low-oxidized antioxidant-supplemented treatment. The results demonstrate that dietary antioxidants can minimize the oxidative instability of proteins and lipids, and the protection may be linked to improved cellular antioxidant enzymatic activity.

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Section: Resultsmentioning

confidence: 99%

Dietary antioxidant supplementation enhances lipid and protein oxidative stability of chicken broiler meat through promotion of antioxidant enzyme activity

et al. 2014

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“…RNA concentration and purity were estimated from the optical density at 260 and 280 nm, respectively. cDNA synthesis and relative quantification of target gene mRNA compared to the housekeeping gene GAPDH mRNA was determined by real-time detection RT-PCR as described previously [50]. Sequences of gene-specific primers obtained from Operon (Köln, Germany) were as follows (NCBI GenBank; forward, reverse): GAPDH (NM_017008; 5'-GCA TGG CCT TCC GTG TTC C-3', 5'-GGG TGG TCC AGG GTT TCT TAC TC-3'), PPARα (NM_013196; 5'-CCC TCT CTC CAG CTT CCA GCC C-3', 5'-CCA CAA GCG TCT TCT CAG CCA TG-3'), CYP4A1 (M14972; 5'-CAG AAT GGA GAA TGG GGA CAG C-3', 5'-TGA GAA GGG CAG GAA TGA GTG G-3'), ACO (J02752; 5'-CTT TCT TGC TTG CCT TCC TTC TCC-3', 5'-GCC GTT TCA CCG CCT CGT A-3'), L-CPT I (NM_031559; 5'-GGA GAC AGA CAC CAT CCA ACA TA-3', 5'-AGG TGA TGG ACT TGT CAA ACC-3'), MCAD (NM_016986; 5'-CAA GAG AGC CTG GGA ACT TG-3', 5'-CCC CAA AGA ATT TGC TTC AA-3'), LCAD (NM_012819; 5'-AAG GAT TTA AGG GCA AGA AGC-3', 5'-GGA AGC GGA GGC GGA GTC-3'), SREBP-1c (XM_213329; 5'-GGA GCC ATG GAT TGC ACA TT-3', 5'-AGG AAG GCT TCC AGA GAG GA-3'), FAS (NM_017332; 5'-AGG TGC TAG AGG CCC TGC TA-3', 5'-GTG CAC AGA CAC CTT CCC AT-3'), c-myc (NM_012603; 5'-CTG GAG TGA GAA GGG CTT TG-3', 5'-CAG CAG CTC GAA TTT CTT CC-3'), c-jun (NM_021835; 5'-ACC AAG AAT TCC GTG ACG AC-3', 5'-CAA GGT CAT GCT CTG CTT CA-3'), and c-fos (NM_022197; 5'-CAT CGG CAG AAG GGG CAA AGT AGA G-3', 5'-TGC CGG AAA CAA GAA GTC ATC AAA G-3').…”

Section: Methodsmentioning

confidence: 99%

Feeding oxidized fat during pregnancy up-regulates expression of PPARα-responsive genes in the liver of rat fetuses

et al. 2007

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BackgroundFeeding oxidized fats causes activation of peroxisome proliferator-activated receptor α (PPARα) in the liver of rats. However, whether feeding oxidized fat during pregnancy also results in activation of PPARα in fetal liver is unknown. Thus, this study aimed to explore whether feeding oxidized fat during pregnancy causes a PPARα response in fetal liver. Two experiments with pregnant rats which were administered three different diets (control; oxidized fat; clofibrate as positive control) in a controlled feeding regimen during either late pregnancy (first experiment) or whole pregnancy (second experiment) were performed.ResultsIn both experiments pregnant rats treated with oxidized fat or clofibrate had higher relative mRNA concentrations of the PPARα-responsive genes acyl-CoA oxidase (ACO), cytochrome P450 4A1 (CYP4A1), L-type carnitin-palmitoyl transferase I (L-CPT I), medium-chain acyl-CoA dehydrogenase (MCAD), and long-chain acyl-CoA dehydrogenase (LCAD) in the liver than control rats (P < 0.05). In addition, in both experiments fetuses of the oxidized fat group and the clofibrate group also had markedly higher relative mRNA concentrations of ACO, CYP4A1, CPT I, MCAD, and LCAD in the liver than those of the control group (P < 0.05), whereas the relative mRNA concentrations of PPARα, SREBP-1c, and FAS did not differ between treatment groups. In the second experiment treatment with oxidized fat also reduced triacylglycerol concentrations in the livers of pregnant rats and fetuses (P < 0.05).ConclusionThe present study demonstrates for the first time that components of oxidized fat with PPARα activating potential are able to induce a PPARα response in the liver of fetuses. Moreover, the present study shows that feeding oxidized fat during whole pregnancy, but not during late pregnancy, lowers triacylglycerol concentrations in fetal livers.

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“…In the liver, the role of ACSL1 is not known, but changes observed in mRNA abundance generally coincide with reported changes in oxidative genes. For example, dietary supplementation with polyunsaturated fatty acids, overexpression of hepatocyte nuclear factor 4-a, and several PPARa agonists all increase the mRNA of ACSL1 as well as that of oxidative genes such as carnitine palmitoyl transferase-1 and acyl-CoA oxidase (9,(18)(19)(20). It seems likely that ACSL1 mRNA is under a complex regulatory control that is probably tissue specific.…”

Section: Discussionmentioning

confidence: 99%

Rat long-chain acyl-CoA synthetase mRNA, protein, and activity vary in tissue distribution and in response to diet

Mashek¹,

Coleman

2006

Journal of Lipid Research

168

135

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Distinct isoforms of long-chain acyl-CoA synthetases (ACSLs) may partition fatty acids toward specific metabolic cellular pathways. For each of the five members of the rat ACSL family, we analyzed tissue mRNA distributions, and we correlated the mRNA, protein, and activity of ACSL1 and ACSL4 after fasting and refeeding a 69% sucrose diet. Not only did quantitative real-time PCR analyses reveal unique tissue expression patterns for each ACSL isoform, but expression varied markedly in different adipose depots. Fasting increased ACSL4 mRNA abundance in liver, muscle, and gonadal and inguinal adipose tissues, and refeeding decreased ACSL4 mRNA. A similar pattern was observed for ACSL1, but both fasting and refeeding decreased ACSL1 mRNA in gonadal adipose. Fasting also decreased ACSL3 and ACSL5 mRNAs in liver and ACSL6 mRNA in muscle. Surprisingly, in nearly every tissue measured, the effects of fasting and refeeding on the mRNA abundance of ACSL1 and ACSL4 were discordant with changes in protein abundance. These data suggest that the individual ACSL isoforms are distinctly regulated across tissues and show that mRNA expression may not provide useful information about isoform function. They further suggest that translational or posttranslational modifications are likely to contribute to the regulation of ACSL isoforms.-Mashek, D. G., L. O. Li, and R. A. Coleman. Rat long-chain acyl-CoA synthetase mRNA, protein, and activity vary in tissue distribution and in response to diet.

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Effects of dietary fat and oxidized cholesterol on gene expression in rat liver as assessed by cDNA expression array analysis

Abstract: The present study showed that dietary oxidized cholesterol transcriptionally affects many genes involved in xenobiotic metabolism and stress response--an effect that was amplified by the administration of fish oil as dietary fat.

Cited by 9 publications

References 40 publications

Dietary antioxidant supplementation enhances lipid and protein oxidative stability of chicken broiler meat through promotion of antioxidant enzyme activity

Dietary antioxidant supplementation enhances lipid and protein oxidative stability of chicken broiler meat through promotion of antioxidant enzyme activity

Feeding oxidized fat during pregnancy up-regulates expression of PPARα-responsive genes in the liver of rat fetuses

Rat long-chain acyl-CoA synthetase mRNA, protein, and activity vary in tissue distribution and in response to diet

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