Effects of Dietary Fatty Acids on Bovine Oocyte Competence and Granulosa Cells

Sharma, Aditya Kumar; Baddela, Vijay Simha; Roettgen, Volker; Vernunft, Andreas; Viergutz, Torsten; Dannenberger, Dirk; Hammon, H.M.; Schoen, Jennifer; Vanselow, Jens

doi:10.3389/fendo.2020.00087

Cited by 19 publications

(16 citation statements)

References 58 publications

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“…Among other dietary FA, rumenic acid increases plasma concentration of insulin-like growth factor-1 (IGF-1) in cows and consequently promoting conception rates [379]. Recently, CLA was reported to affect follicular granulosa cells morphology and function, which may result in a compromised ovarian cyclicity and impaired fertility [380].…”

Section: Additional Effectsmentioning

confidence: 99%

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

Bionaz

Vargas‐Bello‐Pérez

Busato

2020

J Animal Sci Biotechnol

104

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High producing dairy cows generally receive in the diet up to 5–6% of fat. This is a relatively low amount of fat in the diet compared to diets in monogastrics; however, dietary fat is important for dairy cows as demonstrated by the benefits of supplementing cows with various fatty acids (FA). Several FA are highly bioactive, especially by affecting the transcriptome; thus, they have nutrigenomic effects. In the present review, we provide an up-to-date understanding of the utilization of FA by dairy cows including the main processes affecting FA in the rumen, molecular aspects of the absorption of FA by the gut, synthesis, secretion, and utilization of chylomicrons; uptake and metabolism of FA by peripheral tissues, with a main emphasis on the liver, and main transcription factors regulated by FA. Most of the advances in FA utilization by rumen microorganisms and intestinal absorption of FA in dairy cows were made before the end of the last century with little information generated afterwards. However, large advances on the molecular aspects of intestinal absorption and cellular uptake of FA were made on monogastric species in the last 20 years. We provide a model of FA utilization in dairy cows by using information generated in monogastrics and enriching it with data produced in dairy cows. We also reviewed the latest studies on the effects of dietary FA on milk yield, milk fatty acid composition, reproduction, and health in dairy cows. The reviewed data revealed a complex picture with the FA being active in each step of the way, starting from influencing rumen microbiota, regulating intestinal absorption, and affecting cellular uptake and utilization by peripheral tissues, making prediction on in vivo nutrigenomic effects of FA challenging.

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Section: Additional Effectsmentioning

confidence: 99%

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

Bionaz

Vargas‐Bello‐Pérez

Busato

2020

J Animal Sci Biotechnol

104

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“…However, in recent research, OA is reported to have adverse impacts on the steroidogenesis and morphology of bovine granulosa cells cultured in vitro [ 54 ]. Similarly, in vitro culture experiment involving bovine follicular granulosa cells revealed that α-linolenic acid (ALA) and LA inhibit the expression of genes associated with steroidogenesis in granulosa cells and lower the concentrations of secreted estradiol and progesterone [ 55 ]. But, the treatment of bovine granulosa cells with another functional fatty acid, DHA (docosahexaenoic acid), promoted granulosa cell proliferation as well as progesterone and estradiol secretion [ 56 ].…”

Section: Metabolism Of Functional Fatty Acids In Livestockmentioning

confidence: 99%

Role of functional fatty acids in modulation of reproductive potential in livestock

Zeng

Liu

et al. 2023

J Animal Sci Biotechnol

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Fatty acids are not only widely known as energy sources, but also play important roles in many metabolic pathways. The significance of fatty acids in modulating the reproductive potential of livestock has received greater recognition in recent years. Functional fatty acids and their metabolites improve follicular development, oocyte maturation and embryo development, as well as endometrial receptivity and placental vascular development, through enhancing energy supply and precursors for the synthesis of their productive hormones, such as steroid hormones and prostaglandins. However, many studies are focused on the impacts of individual functional fatty acids in the reproductive cycle, lacking studies involved in deeper mechanisms and optimal fatty acid requirements for specific physiological stages. Therefore, an overall consideration of the combination and synergy of functional fatty acids and the establishment of optimal fatty acid requirement for specific stages is needed to improve reproductive potential in livestock.

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“…Dietary lipid supplementation is believed to enhance fertilizing potential in animals by increasing ovulatory follicle size, progesterone concentration in plasma and lifespan of corpus luteum (Sharma et al, 2020). Supplementing ruminants' diet with fats improves quality of the blastocysts obtained.…”

Section: Introductionmentioning

confidence: 99%

Synergistic impact of α-linolenic acid and α-tocopherol on in vitro maturation and culture of buffalo oocytes

et al. 2024

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The objective of the current study was to investigate the synergistic impact of α-Tocopherol and α-Linolenic acid (100 µM) on IVM and IVC of Nili Ravi buffalo oocytes. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. Buffalo cumulus oocyte complexes were placed randomly in the five experimental groups included; GROUP 1: Maturation media (MM) + 100 µM ALA (control), GROUP 2: MM + 100 µM ALA + 50μM α-Tocopherol, GROUP 3: MM + 100 µM ALA + 100μM α-Tocopherol, GROUP 4: MM + 100 µM ALA + 200 μM α-Tocopherol and GROUP 5: MM + 100 µM ALA + 300 μM α-Tocopherol under an atmosphere of 5% CO2 in air at 38.5 °C for 22-24 h. Cumulus expansion and nuclear maturation status was determined (Experiment 1). In experiment 2, oocytes were matured as in experiment 1. The matured oocytes were then fertilized in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h and cultured in synthetic oviductal fluid (SOF) medium to determine effect of α-Linolenic acid (100 µM) and α-Tocopherol in IVM medium on IVC of presumptive zygotes. To study the effect of α-Linolenic acid (100 µM) in IVM media and increasing concentration of α-tocopherol in the culture media on early embryo development (Experiment 3), the presumptive zygotes were randomly distributed into the five experimental groups with increasing concentration of α-tocopherol in culture media. Higher percentage of MII stage oocytes in experiment 1(65.2±2.0), embryos at morula stage in experiment 2 (30.4±1.5) and experiment 3 (22.2±2.0) were obtained. However, overall results for cumulus cell expansion, maturation of oocyte to MII stage and subsequent embryo development among treatments remain statistically similar (P > 0.05). Supplementation of α-tocopherol in maturation media having α-Linolenic acid and/or in embryo culture media did not further enhance in vitro maturation of oocyte or embryo production.

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Effects of Dietary Fatty Acids on Bovine Oocyte Competence and Granulosa Cells

Cited by 19 publications

References 58 publications

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

Role of functional fatty acids in modulation of reproductive potential in livestock

Synergistic impact of α-linolenic acid and α-tocopherol on in vitro maturation and culture of buffalo oocytes

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