Effects of dietary gelatin hydrolysates on bone mineral density in magnesium-deficient rats

Abstract

BackgroundThe major types of commercially available gelatin hydrolysates are prepared from mammals or fish. Dietary gelatin hydrolysates from mammals were reported to improve bone mineral density (BMD) in some animal models. In contrast, there is limited study showing the effects of dietary gelatin hydrolysates from fish on BMD. The quantity and structure of peptides in the plasma after oral administration of gelatin hydrolysates depend on the gelatin source, which suggests that the biological activity of gelatin hydrolysates depend on the gelatin source. This study examined the effects of fish-derived gelatin hydrolysate (FGH) or porcine-derived gelatin hydrolysate (PGH) intake on BMD and intrinsic biomechanical properties in magnesium (Mg)-deficient rats as a model showing the decrease in both BMD and intrinsic biomechanical properties.MethodsFour-week-old male Wistar rats were assigned into four groups: a normal group was fed a normal diet (48 mg Mg/100 g diet), a Mg-deficient (MgD) group was fed a MgD diet (7 mg Mg/100 g diet), a FGH group was fed a MgD + FGH diet (5% FGH), and a PGH group was fed a MgD + PGH diet (5% PGH) for 8 weeks. At the end of the study, BMD and intrinsic biomechanical properties of the femur were measured.ResultsThe MgD group showed significantly lower Young’s modulus, an intrinsic biomechanical property, and trabecular BMD of the femur than the normal group; however, the MgD diet did not affect cortical BMD and cortical thickness. Both the FGH and the PGH groups showed significantly higher cortical thickness and ultimate displacement of the femur than the normal group, but neither type of gelatin hydrolysate affected Young’s modulus. Furthermore, the FGH group, but not the PGH group, showed significantly higher trabecular BMD than the MgD group.ConclusionsThis study indicates that FGH and PGH increase cortical thickness but only FGH prevents the decrease in trabecular BMD seen in Mg-deficient rats, while neither type of gelatin hydrolysate affect intrinsic biomechanical properties.

Collagen Hydrolysate against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study

“…Morphometric measurements were supportive to the histological findings; there was significant increase in the mean thickness of compact bone and in the mean area of trabecular bone when compared to FM groups (II and IV). This is in accordance with an earlier study, which investigated the effect of CH in magnesium deficient OP rats and proved that it could attenuate bone brittleness by increasing cortical thickness [2] . Similar finding was reported by Liu et al [21] who stated that CP supplementation could inhibit bone loss with significant increase in the trabecular bone area in ovariectomized OP rats.…”

“…Osteoporosis (OP) is a globally growing health problem affecting over 200 million people worldwide and the annual incidence of hip fracture due to this disease is about 1.7 million worldwide [1] . It is characterized by deterioration of bone tissue and low bone mass because of enhanced bone resorption that is not compensated by enhanced bone formation, increasing fracture risk [2] . Primary OP is associated with aging and is more common in women especially postmenopausal due to decreased estrogen.…”

Valorization of fish byproducts: Sources to end‐product applications of bioactive protein hydrolysate

“…To support bone health, a number of studies focused on the supplementation of FPH to the individual have been carried out. In their study, Noma et al (2017) evaluated the effect of FPH (5%) on bone mineral density in magnesium (Mg)-deficient male Wistar rats in comparison with porcine gelatin hydrolysate (PGH, 5%) and control (48 mg Mg/100 g diet) during 8 weeks of feeding trial. The result showed that both FPH and PGH increase cortical thickness in Mg-deficient rats.…”

Gelatin extraction from fish waste and potential applications in food sector

“…Another study was conducted by Norma, in which fish gelatin was used to minimise the bone brittleness. The results showed that fish gelatin has huge potential in order to attenuate the bone brittleness (Noma et al ., 2017). Fish gelatin could be utilised as a potential anti‐hypertensive agent in the diet of people who are affected by hypertensive condition which is a major risk for cardiovascular diseases (Kearney et al ., 2005; Chen et al ., 2018).…”