This study investigates the impact of Juglans kernel powder (JKP) and Jacobinia leaf powder (JLP) supplementation on Aflatoxin-B1 (AF) exposed broiler chickens. 200 Cobb-500 broiler chicks were grouped to four treatment: CONT: No supplement; AFNS: 0.5 mg/kg AF; AFJK: 0.5 mg/kg AF+ 350 mg/kg JKP; AFJL: 0.5 mg/kg AF+350 mg/kg JLP. On day 42, the broiler chicken’s relative growth rate, and dressed percentage were lowest in AFNS compared to the rest treatments. Meat cholesterol was lower in AFNS, AFJK, and AFJL, compared to CONT. Meat catalase in AFNS was lower than those in CONT, AFJK, and AFJL. Meat glutathione peroxidase levels of birds in AFNS are similar to AFJL but were lower than those in CONT, and AFJK. Lipid oxidation, and protein oxidation activities of broiler chickens in AFNS were higher than those in the rest of the treatments. Brain catalase, acetylcholinesterase, and glutathione peroxidase activities of birds in AFNS were lower than CONT, AFJK, and AFJL. Expressions of proinflammatory cytokines, and 8-hydroxy-2'-deoxyguanosine in AFNS were higher compared to other treatments. The immunoglobulins A, E and G of broiler chickens in AFNS were lower than CONT, AFJK, and AFJL. 350 mg/kg JKP or JLP ameliorate the effects of AF contamination on broiler chickens.