Effects of Dietary Lactic Acid Supplementation on the Activity of Digestive and Antioxidant Enzymes, Gene Expressions, and Bacterial Communities in the Intestine of Common Carp, Cyprinus carpio

Hoseini, Seyyed Morteza; Yousefi, Morteza; Afzali-Kordmahalleh, Alireza; Pagheh, Esmaeil; Taheri Mirghaed, Ali

doi:10.3390/ani13121934

Cited by 9 publications

(1 citation statement)

References 90 publications

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“…Consequently, measuring MDA content can be a valuable biomarker to assess the extent of oxidative damage in animals: AlatwinusaYohana et al, 2023. A decline in MDA concentration points to improved oxidative conditions(Hoseini et al, 2023). In this experiment, the serum SOD activity signi cantly increased with higher DDGS levels, while the MDA activity showed the opposite trend.…”

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confidence: 61%

Response of gut microbiota, antioxidation, and disease resistance to Pacific shrimp fed distiller’s dried grains with solubles replaced soybean meal

Yohana,

Ray,

Qihui

et al. 2024

Aquacult Int

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This experiment aimed to examine the potential of distillers dried grains soluble (DDGS) meal as a substitute for soybean meal (SBM) in the Paci c shrimp diet. The study aimed to investigate how DDGS affected disease resistance, antoxidation, and gut microbiota. 840 shrimp were given varied diets with varying concentrations of DDGS. D1 (0%), D2 2%, D3 4%, D4 6%, D5 8%, D6 10%, and D7 12% were the DDGS levels. Each group had three replicates of the 56-day experiment. Initially, the weight was 0.19 ± 0.00 g. The survival rate was consistently 100% for shrimp-fed DDGS, except for group 7 (14%) and the control group, where shrimp mortality was observed. The total protein (TP) content was improved as the inclusion level of DDGS increased. The glucose (GLU) exhibited a downward trend with higher DDGS inclusion.Similarly, the TG and CHO levels in the serum decreased as the DDGS levels increased. The activities of ALT (alanine transaminase) and AST (aspartate aminotransferase) decreased as the dietary DDGS increased, with the control group showing signi cantly higher ALT and AST activities than those fed DDGS diets (P < 0.05). Furthermore, the SOD (superoxide dismutase) activity signi cantly increased with higher DDGS levels, while the MDA (malondialdehyde) activity showed the opposite trend. Notably, the control exhibited the highest MDA activity value. The most prevalent phylu found in the gut microbiota were Proteobacteria, and Bacteroidetes. Upon completion of a 7-day bacteria challenge, it was observed that shrimp fed with the control diet had a lower mortality rate compared to those fed the DDGS diet. Based on the parameters measured in this study, it was determined that replacing 10% of SBM with DDGS in the feed of Paci c shrimp is feasible. The calculation for shrimp survivalSurvival rate % = [100× End number of shrimp/ Initial number of shrimp] Serum biochemical analysisThis study set out to look into the antioxidant activity and serum biochemical parameters of Paci c shrimp. The levels of TG, CHO, GLU, TP, ALT, AST, MDA, and SOD were among the many activities that our team examined. We used assay kits from the prestigious Nanjing Jiancheng Bioengineering Institute in China to guarantee precise and trustworthy results. We used commercial assay kits from the Nanjing Jiancheng Institute in Nanjing, China, to measure ALT and AST levels. The biuret method was used to determine TP levels. SOD activity was measured using the nitro blue tetrazolium reaction, with the color extracted into butanol and measured at 550 nm. The TBA (thiobarbituric acid) test was used to measure the amount of MDA in the hepatopancreas. Lipid hydroperoxide breakdown was also measured using TBA, resulting in red chemicals at the 532 nm absorbance peak. The analysis of digestive enzymeTo conduct a thorough analysis, we studied how dietary DDGS affects the activity of digestive enzymes, speci cally amylase, lysine, and protease, in shrimp. We selected three shrimp for each group and carefully removed and dissected their intestines. The intestine...

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confidence: 61%

Response of gut microbiota, antioxidation, and disease resistance to Pacific shrimp fed distiller’s dried grains with solubles replaced soybean meal

Yohana,

Ray,

Qihui

et al. 2024

Aquacult Int

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Utilizing Fermented Foods in Traditional Therapies for Addressing Metabolic Disorders

Gaur

2024

Traditional Foods: The Reinvented Superfoods

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Unchanged water stress induces growth retardation, histopathological alterations, and antioxidant-immune disruptions in Oreochromis niloticus: the promising role of dietary organic acids

Abdel-Aziz,

El Basuini,

Sadek

et al. 2024

Aquacult Int

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Legal restrictions and consumer trends are contributing to the growing movement for fish welfare. Therefore, the aquaculture sector is a means of reducing animal stress and enhancing physiological state, which raises financial profits. Feed additives have therefore been suggested as possible dietary stress alleviation. In this trend, a 75-day feeding trial was conducted to evaluate the effects of three organic acids (formic acid (FA), lactic acid (LA), and commercial organic acids mix (COM)) as dietary additives in Nile tilapia (Oreochromis niloticus) reared under a stressful condition (unchanged water). Fish (weighing 3.95 ± 0.05 g) were randomly housed into eight groups (n = 210 fish/group; 70 fish/replicate; 3 replicates/group). The first (negative control) and second (positive control) groups were fed a basal diet without additives and with water exchange at 20% of the water volume every 2 days for the first group and without water exchange for the second. The other six groups (FA1, FA2, LA1, LA2, COM1, and COM2) were fed basal diets supplemented with OAs at two doses (1 and 2%) without water exchange during the trial. The results revealed that fish kept without water exchange (positive control) had the highest level of water-unionized ammonia and nitrite and a marked decline in growth performance (weight gain and specific growth rate). The unchanged water induced substantial decreases in the hematological profile (white and red blood cell count, hemoglobin value, and hematocrit %), protein profile indices (total protein, albumin, and globulin), and antioxidant-immune response (superoxide dismutase, catalase, lysozyme, and total immunoglobulin M). In addition, the biochemical indices (glucose, creatinine, urea, alanine aminotransferase, and aspartate aminotransferase) and lipid peroxide (malondialdehyde) were substantially increased in the positive control group. In addition to that, marked and severe histopathological alteration in the gills, liver, and intestine including hyperplasia, hemorrhage, and degenerative and necrotic changes that were associated with desquamation and sloughing were obvious in the PC group. Dietary LA1 significantly enhanced (P < 0.05) the overall measured indices; meanwhile, the dietary FA and COM recorded the worst results. Overall, our findings demonstrate that the unchanged water negatively affected the growth and physiological functions of Nile tilapia. Dietary incorporation of organic acids did not protect the fish from these hazards except the LA (1%). The latter can reduce nitrogenous compounds, enhance immune response, and alleviate these alterations for the sustainable aquaculture industry.

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Effects of Dietary Lactic Acid Supplementation on the Activity of Digestive and Antioxidant Enzymes, Gene Expressions, and Bacterial Communities in the Intestine of Common Carp, Cyprinus carpio

Cited by 9 publications

References 90 publications

Response of gut microbiota, antioxidation, and disease resistance to Pacific shrimp fed distiller’s dried grains with solubles replaced soybean meal

Response of gut microbiota, antioxidation, and disease resistance to Pacific shrimp fed distiller’s dried grains with solubles replaced soybean meal

Utilizing Fermented Foods in Traditional Therapies for Addressing Metabolic Disorders

Unchanged water stress induces growth retardation, histopathological alterations, and antioxidant-immune disruptions in Oreochromis niloticus: the promising role of dietary organic acids

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