Effects of dietary methionine on growth performance and metabolism through modulating nutrient-related pathways in largemouth bass (Micropterus salmoides)

Wang, Wenqiang; Yang, Peng; He, Chaoqun; Chi, Shuyan; Li, Songlin; Mai, Kangsen; Song, Fei

doi:10.1016/j.aqrep.2021.100642

Cited by 24 publications

(20 citation statements)

References 59 publications

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“…Correspondingly, as the key downstream factors of TOR, S6K1 and 4EBP1 mRNA levels were upregulated significantly in the 33.68% dietary protein group, which was corresponded to growth performance. As with the results of Wang et al (2021) on largemouth bass ( Micropterus salmoides ), methionine supplementation stimulated growth performance by regulating the TOR signalling pathway including the upregulation of TOR, S6K1 and 4EBP1 mRNA levels. In addition, as reported, the effects of dietary protein levels on growth performance are likely to be involved in the activation of the TOR signalling pathway (Gao et al, 2019; Qin et al, 2019).…”

Section: Discussionsupporting

confidence: 78%

The optimum dietary protein requirement of the genetically improved farmed tilapia ( GIFT : Oreochromis niloticus ): Effects on growth performance and protein metabolism via GH‐IGF axis and TOR signalling pathway at different seasonal growth stages

Huang

Liang

Ren

et al. 2022

Aquaculture Research

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Four experimental trials were conducted to explore the optimal dietary protein requirement level for the genetically improved farmed tilapia (GIFT) at different seasonal growth stages. Six practical diets were prepared with graded protein levels (26.45%, 29.28%, 31.69%, 33.68%, 36.18% and 38.75%, dry basis). In Stage 1 (initial weight 3.00 g), the results showed that the optimal growth performance was observed in the 33.68% dietary protein group. Correspondingly, compared with the lowest (26.45%) or highest (38.75%) dietary protein groups, 33.68% dietary protein significantly upregulated the GH‐IGF axis‐related genes (GH, GHR1, GHR2 and IGF‐1) and TOR signalling pathway‐related genes (TOR, S6K1 and 4EBP1). In Stage 2 (initial weight 43.78 g), 26.45% dietary protein contributed to the optimum growth performance. Besides, the expression levels of GH, GHR2 and IGF‐1 and TOR signalling pathway (S6K1 and 4EBP1) decreased with the increasing dietary protein levels, and the maximum values were observed in the 26.45% dietary protein group. In Stage 3 (initial weight 99.97 g), the best growth performance was found in the 31.69% dietary protein group. In addition, the mRNA expression levels of GH, IGF1, S6K1 and 4EBP1 increased with the increase in dietary protein levels up to 31.69%, then gradually declined. In Stage 4 (initial weight 302.82 g), dietary protein levels did not affect the growth performance. Based on the quadratic regression analysis of the SGR, the optimal dietary protein level of each stage was estimated to be 33.08% (Spring, Stage 1), 26.45% (Summer, Stage 2), 33.06% (Autumn, Stage 3) and 26.45% (Winter, Stage 4) of dry diet.

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Section: Discussionsupporting

confidence: 78%

The optimum dietary protein requirement of the genetically improved farmed tilapia ( GIFT : Oreochromis niloticus ): Effects on growth performance and protein metabolism via GH‐IGF axis and TOR signalling pathway at different seasonal growth stages

Huang

Liang

Ren

et al. 2022

Aquaculture Research

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“…An imbalanced amino acids provision has been shown to cause the activation of AAR pathway, which consist of the phosphorylation of eIF2α to suppress protein synthesis, and the activation of the expression of ATF4 to adapt to dietary stress ( 79 , 80 ). Previous studies in teleosts have confirmed the activation of AAR pathway by an imbalanced dietary amino acids supply ( 16 , 17 , 44 ), and it has also been shown that the AAR pathway can be regulated at the transcriptional levels ( 34 ; 18 ). In the present study, although the expression of eIF2α was not influenced, the expression of ATF4 was significantly decreased by the inclusion of protein hydrolysates, indicating an inhibitory action of protein hydrolysates on the AAR pathway in larval largemouth bass.…”

Section: Discussionmentioning

confidence: 77%

“…Diets were manufactured following previously described procedures ( 33 ), and then the dried diets were ground to micro-diets with particle size ranged from 0.18 mm to 0.83 mm. The feeding management procedures for the use of experimental diets with different size was implemented according to the procedures previously described by Wang et al ( 34 ). All diets were packed in double sealed plastic bags, and stored at -20°C until use.…”

Section: Methodsmentioning

confidence: 99%

Functional Properties of Protein Hydrolysates on Growth, Digestive Enzyme Activities, Protein Metabolism, and Intestinal Health of Larval Largemouth Bass (Micropterus salmoides)

Sheng

Turchini

et al. 2022

Front. Immunol.

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The present study was conducted to investigate the effects of dietary inclusion of protein hydrolysates on growth performance, digestive enzyme activities, protein metabolism, and intestinal health in larval largemouth bass (Micropterus salmoides). The experimental feeding trial presented in this study was based on five isonitrogenous and isolipidic diets formulated with graded inclusion levels of protein hydrolysates, and it showed that protein hydrolysates improved growth performance, reduced larval deformity rate, and increased the activity of digestive enzymes, including pepsin and trypsin. Gene expression results revealed that the supplementation of protein hydrolysates upregulated the expression of intestinal amino acid transporters LAT2 and peptide transporter 2 (PepT2), as well as the amino acid transporters LAT1 in muscle. Dietary provision of protein hydrolysates activated the target of rapamycin (TOR) pathway including the up-regulation of TOR and AKT1, and down-regulation of 4EBP1. Additionally, the expression of genes involved in the amino acids response (AAR) pathway, ATF4 and REDD1, were inhibited. Protein hydrolysates inhibited the transcription of some pro-inflammatory cytokines, including IL-8 and 5-LOX, but promoted the expression of anti-inflammatory cytokines TGF-β and IL-10. The 16S rRNA analysis, using V3-V4 region, indicated that dietary protein hydrolysates supplementation reduced the diversity of the intestine microbial community, increased the enrichment of Plesiomonas and reduced the enrichment of Staphylococcus at the genus level. In summary, protein hydrolysates have been shown to be an active and useful supplement to positively complement other protein sources in the diets for largemouth bass larvae, and this study provided novel insights on the beneficial roles and possible mechanisms of action of dietary protein hydrolysates in improving the overall performance of fish larvae.

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“…We envisaged that the changes of free AA profile after 60% FM replaced by CSM might be partially due to the unbalanced AA composition of higher CSM containing diets [ 50 , 51 ]. A lower level of free AA in muscle not only decreased the bricks for protein synthesis and antioxidative capacity but also disordered the glycolipid metabolism [ 52 , 53 ]. Moreover, the present study also demonstrated that the CSM 60 diet significantly decreased the glycine and tyrosine concentration more than the CSM0 diet.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, GH and its downstream mediator, IGF-1, constructed a pleotropic axis through the TOR signaling pathway regulating growth, metabolism and organ function [ 75 , 76 ]. Recent studies from our laboratory reported that in largemouth bass, a dietary AA level could through TOR, AAR and GH-IGF signaling to regulate fish growth performance and glycolipid metabolism [ 53 ]. Likewise, the present study in golden pompano also demonstrated that dietary protein sources had profound effects on changing nutrient sensing signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Muscle Nutritive Metabolism Changes after Dietary Fishmeal Replaced by Cottonseed Meal in Golden Pompano (Trachinotus ovatus)

Qin

Geng

et al. 2022

Metabolites

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Our previous study demonstrated that based on growth performance and feed utilization, cottonseed meal (CSM) could substitute 20% fishmeal (FM) without adverse effect on golden pompano (Trachinotus ovatus). Muscle deposition was also an important indicator to evaluate the efficiency of alternative protein sources. Therefore, the present study was conducted to explore the changes of physiobiochemical and nutrient metabolism in muscle after FM replaced by CSM. Four isonitrogenous and isolipidic experimental diets (42.5% crude protein, 14.0% crude lipid) were formulated to replace 0% (CSM0 diet), 20% (CSM20 diet), 40% (CSM40 diet), and 60% (CSM60 diet) of FM with CSM. Juvenile fish (24.8 ± 0.02 g) were fed each diet for 6 weeks. The results presented, which, compared with the CSM0 diet, CSM20 and CSM40 diets, had no effect on changing the muscle proximate composition and free essential amino acid (EAA) concentration. For glycolipid metabolism, the CSM20 diet did not change the mRNA expression of hexokinase (hk), glucose transport protein 4 (glut4), glucagon-like peptide 1 receptor (glp-1r), while over 20% replacement impaired glucose metabolism. However, CSM20 and CSM40 diets had no effect on altering lipid metabolism. Mechanistically, compared with the CSM0 diet, the CSM20 diet did not change muscle nutritive metabolism through keeping the activities of the nutrient sensing signaling pathways stable. Higher replacement would break this balance and lead to muscle nutritive metabolism disorders. Based on the results, CSM could substitute 20–40% FM without affecting the muscle nutritive deposition. All data supplemented the powerful support for our previous conclusion that CSM could successfully replace 20% FM based on growth performance.

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Effects of dietary methionine on growth performance and metabolism through modulating nutrient-related pathways in largemouth bass (Micropterus salmoides)

Cited by 24 publications

References 59 publications

The optimum dietary protein requirement of the genetically improved farmed tilapia ( GIFT : Oreochromis niloticus ): Effects on growth performance and protein metabolism via GH‐IGF axis and TOR signalling pathway at different seasonal growth stages

The optimum dietary protein requirement of the genetically improved farmed tilapia ( GIFT : Oreochromis niloticus ): Effects on growth performance and protein metabolism via GH‐IGF axis and TOR signalling pathway at different seasonal growth stages

Functional Properties of Protein Hydrolysates on Growth, Digestive Enzyme Activities, Protein Metabolism, and Intestinal Health of Larval Largemouth Bass (Micropterus salmoides)

Muscle Nutritive Metabolism Changes after Dietary Fishmeal Replaced by Cottonseed Meal in Golden Pompano (Trachinotus ovatus)

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