Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology

Abstract

The effects of Paenibacillus xylanexedens, alone or in combination with inulin and lactulose were evaluated on growth performance and intestinal histomorphology of male broilers. A total of 168 one-day-old male broiler chickens were randomly allocated to 4 experimental groups as follows: C, basal diet (control; without supplementation); P, 1×10 9 cfu/kg-feed P. xylanexedens; IN, 1×10 9 cfu/kg-feed P. xylanexedens and 1% inulin; LAC, 1×10 9 cfu/kg-feed P. xylanexedens and 0.5% lactulose. Addition of P. xylanexedens and synbiotics significantly increased broiler body weight gain (P<0.001) and feed conversion ratio (P=0.023) from 1 to 21 day of age. Moreover, a significant increase in body weight gain was observed when birds were fed with synbiotic supplemented diets during the overall experimental period (P=0.013). The addition of probiotic and synbiotics increased (P=0.047) villus height in ileum on day 42. Our results indicate that P. xylanexedens can be used as a probiotic feed additive in broiler diets and can improve broiler performance and intestinal histomorphology. Broyler rasyonlarında probiyotik ve sinbiyotik kullanılmasının broyler performans ve bağırsak histomorfolojisi üzerine etkisiÖzet: Paenibacillus xylanexedens'in tek başına veya inulin veya laktuloz ile birlikte kullanılmasının erkek broylerlerin büyüme performansı ve bağırsak histomorfolojisi üzerine olan etkisi incelendi. Toplamda 168 adet günlük erkek civciv dört deneme grubuna ayrıldı: C, bazal rasyon (kontrol); P, 1×10 9 cfu/kg-yem P. xylanexedens; IN, 1×10 9 cfu/kg-yem P. xylanexedens ve %1 inulin; LAC, 1×10 9 cfu/kg-yem P. xylanexedens ve %0.5 laktuloz. P. xylanexedens ve sinbiyotik ilavesinin 1-21. günlerde broyler canlı ağırlık artışını (P<0.001) ve yemden yararlanma oranını (P=0.023) önemli düzeyde arttırdığı sonucuna varıldı. Tüm araştırma periyodu dikkate alındığında sinbiyotik ilavesinin canlı ağırlık artışını önemli ölçüde (P=0.013) etkilediği görüldü. Araştırmanın 42. gününde probiyotik ve sinbiyotik ilave edilen gruplarda ileum villus yüksekliğinin daha fazla olduğu tespit edildi (P=0.047). Sonuç olarak, P. xylanexedens'in broyler büyüme performansını ve bağırsak histomorfolojisini olumlu yönde etkilediği ve rasyonlarında probiyotik yem katkı maddesi olarak kullanılabileceği görüldü.

Effect of different synbiotic administration methods on growth, carcass characteristics, ileum histomorphometry, and blood biochemistry of Cobb-500 broilers

“…In birds fed probiotic and synbiotic diets, the ileum villus height was significantly greater on day 21 compared to the control. On day 42, the ratio of VD to CD in birds fed synbiotic diets [48] was uniformly greater, corroborating this research. The broilers given 2.5 g/kg of synbiotic through in feed administration had significantly longer villi than the other groups [75].…”

“…Synbiotics promote intestinal health, enhance immune function, and favorably alter the intestinal microbiota. Synbiotics boost beneficial bacteria populations, such as Lactobacillus and Bifidobacterium, and concurrently minimize harmful bacteria, such as Clostridium and Escherichia coli, leading to mortality rate reduction [28,[44][45][46][47][48].…”

Hayvansal Yağın Yağ Asitleri Kalsiyum Tuzunun Broyler Performansı, İç Yağın Yağ Asidi Kompozisyonu, Kemiklerin Biyomekanik Özellikleri ve Tibia Mineral Düzeyi Üzerine Etkisi

“…The tissue samples for histomorphological analysis were taken from the mid part of the duodenum, jejunum, and ileum. Afterwards, the tissue samples were flushed with saline solution to remove adherent intestinal contents and fixed in 10% neutral buffered formalin solution for 24 h [20] . Then the carcasses were manually dissected and weighed individually to calculate carcass percentage.…”

Microstructure of the small intestine in broiler chickens fed a diet with probiotic or synbiotic supplementation

“…In addition, the mechanism may include competition for nutrients and adhesion receptors to the intestinal epithelium (Lee, Lillehoj, & Siragusa, 2010;Yang, Iji, & Choct, 2009). Moreover, probiotics can improve the integrity of the gastrointestinal mucosa and the digestibility of nutrients and thus improve the performance of animals, and even muscle microstructure (van der Aar, Molist, & van der Klis, 2017; Awad, Ghareeb, & Böhm, 2008Bogucka, Ribeiro, da Costa, & Bednarczyk, 2018;Çalik, Ekim, Bayraktaroğlu, Ergűn, & Saçakli, 2017;Pelicano et al, 2005;Pereira et al, 2018). According to Gibson, Probert, Loo, Rastall, and Roberfroid (2004), prebiotics are selectively fermented ingredients that allow specific changes in both the composition and activity of the intestinal microflora.…”