“…Similar findings were also reported in the muscle proximate composition of Nile tilapia [21][22]. Another experiment in which fishmeal was used as the main protein source to study the dietary protein requirement of fish exhibited that body ash content was not affected by the dietary protein level [23][24]. The lipid level was observed to decrease in the O. niloticus, when fed with the diet containing 20% replacement of fishmeal in feed B.…”
“…Similar findings were also reported in the muscle proximate composition of Nile tilapia [21][22]. Another experiment in which fishmeal was used as the main protein source to study the dietary protein requirement of fish exhibited that body ash content was not affected by the dietary protein level [23][24]. The lipid level was observed to decrease in the O. niloticus, when fed with the diet containing 20% replacement of fishmeal in feed B.…”
“…Feces were stripped from all fish by applying gentle pressure in the anal area according to the procedure of our laboratory study [40]. Fecal samples were collected and then stored at À20 C until used as described by Yang et al [41].…”
Section: Digestibility Trial and Sample Collectionmentioning
“…According to Ballantyne (2001) and Stone et al (2003), the amino acid surplus from protein-rich diets cannot be directly stored in fishes and they are deaminated and converted into energetic compounds. The augmented protein breakdown in fish results in an increased concentration of plasma ammonia, which is promptly excreted through the gills into the environment (van Waarde 1983;Yang et al 2002).…”
Section: Discussionmentioning
confidence: 99%
“…Dietary protein can affect growth and health of cultured fish as well as maintenance of a good farming environment (Rahman et al 2008a(Rahman et al , 2008c(Rahman et al , 2012Rahman & Verdegem 2010). Therefore, it should be carefully apportioned to meet the needs of the cultured organisms (Rajkumar et al 2013;Yang et al 2002). If excessive protein is supplied in the diet, only part of it will be used to synthesize new tissues and the surplus will be metabolized as an energy source (Guo et al 2012).…”
The effects of dietary protein level on the growth performance and ammonia excretion of the leopard coral grouper,Plectropomus leopardus were investigated for eight weeks. Fish were fed diets with 40, 45, 50, 55 and 60%
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