Effects of dietary restriction on appendicular bone in the SENCAR mouse

Murray, Éamonn; Beamer, Wesley G.; Duarte, Maria Eugênia Lammoglia; Behnam, Keyvan; Grisanti, Mario; Murray, Samuel S.

doi:10.1053/meta.2001.21694

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…Our experimental model was the aged male Wistar rat, which has been established as a relevant model for age-related bone loss in human subjects 39 . The severity of dietary restriction (40 %) was based on previous rodent studies 17 , 34 , 35 , 40 . During the experimental period, body weight markedly decreased with both dietary restrictions (a 23 % change compared to the controls; Fig.…”

Section: Discussionmentioning

confidence: 99%

Impact of energy and casein or whey protein intake on bone status in a rat model of age-related bone loss

et al. 2007

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In the elderly, nutritional deficiencies, such as low energy and protein intake, are suggested to increase the risk of osteoporotic fractures. Modulation of the amount and quality of protein intake under energy deficient conditions represents an interesting strategy to prevent aged-related bone loss. We investigated the effect of a 5-month dietary restriction on bone status in 16-month-old male rats. Rats were randomised into six groups (n 10 per group). Control animals were fed a normal diet containing either casein (N-C) or whey protein (N-WP). The other groups received a 40 % protein and energy-restricted diet with casein or whey protein (PER-C and PER-WP) or a normal protein and energy-restricted diet (ER-C and ER-WP). Both restrictions (PER and ER) induced a decrease in femoral bone mineral density (BMD), consistent with impaired biomechanical properties and a reduced cortical area at the diaphysis. Plasma osteocalcin and urinary deoxypyridinoline levels suggested a decrease in bone turnover in the PER and ER groups. Interestingly, circulating insulin-like growth factor 1 (IGF-1) levels were also lowered. Overall, normal protein intake did not elicit any bone sparing effect in energy-deficient rats. Regarding protein quality, neither casein nor WP appeared to significantly prevent the BMD decrease. This study confirms that nutritional deficiencies may contribute to osteopenia through decreased IGF-1 levels. Moreover, it seems that impaired bone status could not be significantly prevented by modulating the amount and quality of dietary proteins.

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Section: Discussionmentioning

confidence: 99%

Impact of energy and casein or whey protein intake on bone status in a rat model of age-related bone loss

et al. 2007

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“…( 2 , 3 ) There is also a marked circadian rhythm in bone turnover markers in rodents. ( 16 ) One study using CTX was not able to show an acute effect of fasting versus feeding on CTX; however, in a food fractionation study, changing the pattern of nutrient intake significantly modified bone resorption determined using urinary { 3 H}tetracycline excretion and resulted in a net increase in BMD. ( 8 , 17 , 18 )…”

Section: Acute Effect Of Nutrients On Bone Turnovermentioning

confidence: 99%

Potential Role of Pancreatic and Enteric Hormones in Regulating Bone Turnover

Clowes

Khosla

Eastell

2005

Journal of Bone and Mineral Research

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“…Several studies revealed that exchange of the terminal agmatine residue by uncharged chains improved cell permeability of the inhibitors [90,91] and as P2 amino acid attached to the Eps group entered the hydrophobic S2 pocket of papain‐like enzymes only hydrophobic amino acid residues (e. g., Leu, Ile, Phe) could be used in this position [92–94] . In addition, the proposed binding modes of E‐64 (and its analogue E‐64c) to papain [33] together with docking experiments of the same substrate using X‐ray structure of cathepsin B [95] led to the conclusion that a leucine residue in P2 position was required to occupy S2 pocket [39,96] .…”

Section: Synthesis Of Epoxysuccinates‐based Chemical Probesmentioning

confidence: 99%

Synthetic Approaches of Epoxysuccinate Chemical Probes

et al. 2023

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Synthetic chemical probes are powerful tools for investigating biological processes. They are particularly useful for proteomic studies such as activity‐based protein profiling (ABPP). These chemical methods initially used mimics of natural substrates. As the techniques gained prominence, more and more elaborate chemical probes with increased specificity towards given enzyme/protein families and amenability to various reaction conditions were used. Among the chemical probes, peptidyl‐epoxysuccinates represent one of the first types of compounds used to investigate the activity of the cysteine protease papain‐like family of enzymes. Structurally derived from the natural substrate, a wide body of inhibitors and activity‐ or affinity‐based probes bearing the electrophilic oxirane unit for covalent labeling of active enzymes now exists. Herein, we review the literature regarding the synthetic approaches to epoxysuccinate‐based chemical probes together with their reported applications, from biological chemistry and inhibition studies to supramolecular chemistry and the formation of protein arrays.

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Effects of dietary restriction on appendicular bone in the SENCAR mouse

Cited by 8 publications

References 28 publications

Impact of energy and casein or whey protein intake on bone status in a rat model of age-related bone loss

Impact of energy and casein or whey protein intake on bone status in a rat model of age-related bone loss

Potential Role of Pancreatic and Enteric Hormones in Regulating Bone Turnover

Synthetic Approaches of Epoxysuccinate Chemical Probes

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