Effects of Dietary Supplementation of Copper Soy Proteinate (Cu-SP) and Herbal Mixture (HBM) on the Performance, Blood Parameter and Immune Response in Laying Hens

Abstract

The objective of this experiment was to investigate the effect of dietary supplementation of copper-soy proteinate (Cu-SP) and herbal mixture (HBM) on growth performance, blood parameter, and immune response in laying hens. A total 800 Hy-Line Brown laying hens (60 weeks old) were randomly allotted to 1 of 4 dietary treatments : (1) Control : control diet, (2) Cu-SP : control diet + 100 mg/kg Cu-soy proteinate, (3) HBM : control diet + 0.15% herbal mixture, and (4) Cu-SP + HBM : control diet + 100 mg/kg Cu-soy proteinate + 0.15% herbal mixture. Each treatment was replicated 5 times with forty birds units were arranged according to randomized block design. Feeding trial lasted 5 weeks under 16L : 8D lighting regimen. The diet and water were available ad libitum. Result indicated that during feeding trial of the experiment, hen-day egg production was significantly (P<0.05) higher in Cu-SP and HBM treated groups than control. However, feed intake, feed conversion ratio, egg weight, broken and shell less egg production were not significantly influenced by treatments. Eggshell strength was significantly (P<0.05) higher in Cu-SP than control. Eggshell thickness, eggshell color, egg yolk color, Haugh unit were not significantly influenced by treatments. The level of WBC and stress index (heterophil : lymphocyte) were higher in supplemented groups than the control. The concentration of plasma IgG was higher in supplemented groups than the control. The result of this experiment showed that dietary copper-soy proteinate or herbal mixture tended to improve egg production and affect positively on immune response of laying hens.(Key words : Cu-soy proteinate, Herbal mixture, laying hens, productive laying performance, immune response) † To whom correspondence should be addressed : banght80@korea.kr 서 론 구리(Cu)는 cytochrome oxidase, lysyl oxidase, ceruloplasmin, superoxide dismutase와 같은 수많은 효소들의 작용에 도움을 주는 필수 광물질이다 (Klasing, 1998). 산란계에서의 Cu 요구량은 정확히 제시되어 있지 않지만 (NRC, 1994), 약 리적 수준(sub-therapeutic)으로 100∼300 mg/kg의 사료 내 황산동 형태로 첨가 시 성장 촉진 효과가 있다고 보고되었다 (Klasing, 1988

Effects of Increasing Supplementation of Lycii fructus and Glycyrrhiza uralensis Mixture in Diets on Growth Performance, Blood Parameter, Immune Response and Intestinal Microflora in Broilers

“…본 시험에서는 대조구 (Basal), LF and GU mixture 0.5% 처리구(basal diet+0.5% LF and GU mixture), LF and GU mixture 1.0% 처리구(basal diet+1.0% LF and GU mixture) 총 3처리였으며, 시험사료는 NRC(1994) 요구량에 준하여 CP 22%, ME 3,100 kcal/kg인 육계전기(0 to 21 d)와 CP 19%, ME 3,150 kcal/kg인 육계 후 기(22 to 35 d) 사료를 제조하여 사용하였다. 대조구 사료의 배합비와 영양소 함량은 Lee et al (2013) 은 육계에 감초 첨가 급이 시 증체량과 사료 요구율이 개선 된다고 보고하였으며, 지황, 당귀, 작약, 감초, 오미자, 천궁 으로 구성되어 있는 한방 복합제를 육계와 산란계, 자돈에 게 첨가 급이 시 생산성이 증가한 결과와 일치하였다 (Lee and Paik, 2007;Woo et al, 2007;Kim and Paik, 2008;Kim et al, 2014 (Shon et al, 2004;Lee and Ha, 1994;Oh et al, 1998 …”

Dietary Effects of Herbal Mixture on the Laying Performance, Blood Parameters and Immune Response in Laying Hens

“…또한 감초는 여러 flavonoid로 구성 되어 있는데, 그 중 단맛을 내는 glycyrrhizin (Hanato et al, 2000)이 있으며, 이외에도 Hispaglabridin A, Hispaglabridin B, Glabridin 4-O-methylglabridin, isoprenylchalcone derivative, isoliquiritigenin, formononetin 등의 생리활성 물질을 함유하 고 있다 (Vaya et al, 1997). 약리적 작용으로는 면역 증강, 만성간염, 해독 작용, 강심작용, 간보호 작용, 콜레스테롤 배 출 촉진 작용 등에 효과가 있다고 알려져 있다 (Kakegawa et al, 1992;Kumagai et al, 1967;안덕균, 1998;김창민 등, 1997 (Steel and Torrie, 1980 천궁으로 구성되어 있는 복합제를 육계와 산란계, 자돈에게 첨가 급이시 생산성이 증가한 결과와 일치하였다 (Lee and Paik, 2007;Woo et al, 2007;Kim and Paik, 2008;Kim et al, 2014). Cho et al(2009)은 육계사료에 감초와 용구 혼합 급이시 증체량이 증가한다고 보고하였다.…”