Effects of dietary supplementation of
 Lactobacillus fermentum
 URLP18 on growth, innate immunity and survival against
 Aeromonas hydrophila
 ATCC 7966 challenge in freshwater fish
 Cyprinus carpio
 (common carp)

Govindaraj, Krishnaveni; Samayanpaulraj, Vignesh; Vidhyalakshmi, N.; Velu, Vijay; Uthandakalaipandian, Ramesh

doi:10.1111/are.14974

“…Fish fed supplemented diets also had lower FCR levels. These results are in agreement with previous studies on beneficial effects of dietary pre-, pro-and synbiotics on growth performance parameters, including, diets supplemented with probiotics for juvenile roach (Rutilus rutilus) [58], common carp [59], Nile tilapia (Oreochromis niloticus) [60], Asian sea bass, (Lates calcarifer) [61], white shrimp [62] synbiotic in Texas cichlid (Herichthys cyanoguttatus) [31], and prebiotics in rainbow trout (Oncorhynchus mykiss) [22]. Dietary pre-and probiotics and their combination can stimulate fish appetite and intestinal microflora [63].…”

Section: Discussionsupporting

confidence: 93%

“…Increased nutritional efficiency may be associated with improving digestive enzyme activity and degradation of indigestible nutrients, making them available for adsorption [59]. Probiotic supplements not only colonize intestinal epithelial cells but also adhere to the mucosa [9].…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Supplementation of PrimaLac, Inulin, and Biomin Imbo on Growth Performance, Antioxidant, and Innate Immune Responses of Common Carp (Cyprinus carpio)

Ajdari

¹

,

Ghafarifarsani

²

,

Hoseinifar

³

et al. 2022

Aquaculture Nutrition

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Prebiotics, probiotics, and synbiotics have been successfully used in dietary supplements to achieve aquatic animal health and therefore increase the quality and sustainability of fish production. In the present study, four hundred and twenty common carps, Cyprinus carpio ( 25.37 ± 0.22 g ; mean ± SE ) were randomly attributed to seven treatments, fed with diets containing PrimaLac (probiotic), inulin (prebiotic), and Biomin Imbo (synbiotic), 1 and 2 g/kg for each supplement. After 60 days of feeding, an increase in final weight (FW), weight gain (WG), specific growth rate (SGR), and survival rate (SR) was recorded in the treatment groups compared to that of the control ( P < 0.05 ). The food conversion ratio (FCR) in the treatment groups significantly decreased ( P < 0.05 ). The treated groups showed significant improvements in serum immune parameters: lysozyme (LYZ), alternative complement (ACH50), total immunoglobulin (total Ig), and myeloperoxidase (MPO) ( P < 0.05 ). Feeding fish with supplemented diets significantly showed enhanced antioxidant status: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). Malondialdehyde (MDA) activity was significantly lower in fish fed dietary additives ( P < 0.05 ). Compared with the control group, enzyme parameters revealed that supplementation could significantly decrease alanine transaminase (ALT), alkaline phosphatase (ALP), and aspartate transaminase (AST) ( P < 0.05 ). The biochemical parameters including triglyceride (TRIG), cholesterol (CHO), glucose (GLU), and cortisol (CORT) decreased with dietary supplementation ( P < 0.05 ). Total protein (TP) increased in fish fed experimental diets ( P < 0.05 ). Fish fed pro, pre, and synbiotic exhibited significantly higher digestive enzymes (amylase, lipase, and protease) ( P < 0.05 ). Skin mucus parameters (total Ig, ACH50, LYZ, protease, and ALP) were significantly enhanced in groups fed dietary additives ( P < 0.05 ). Thus, the best recommended inclusion level of supplementation is 2 g/kg for inulin and 2 g/kg for PrimaLac and doses of 1 or 2 g/kg for Biomin Imbo. Dietary PrimaLac, inulin, and Biomin Imbo could be recommended as beneficial feed additives to enhance growth performance, innate immune and antioxidant systems, and promoted biochemical parameters and digestion of common carp; also, the effect of dietary synbiotic was superior to that of prebiotic and probiotic.

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“…Ahmadifar et al (2019) investigated the probiotic role of L. fermentum and obtained better growth and immune responses in the supplemented fish. Similar results were observed in the study of Krishnaveni et al (2021), where a diet containing L. Fermentum improved growth, immunity and resistance against Aeromonas hydrophila in common carp ( Cyprinus carpio ). Besides probiotics, many studies have shown that medicinal plants can also improve fish immunity and resistance to diseases (Awad & Awaad, 2017; Galina et al, 2009; Van Hai, 2015).…”

Section: Introductionsupporting

confidence: 90%

The synergistic effects of the probiotic ( Lactobacillus fermentum ) and cinnamon, Cinnamomum sp. powder on growth performance, intestinal microbiota, immunity, antioxidant defence and resistance to Yersinia ruckeri infection in the rainbow trout ( Oncorhynchus mykiss ) under high rearing density

Jasim

¹

,

Hafsan²,

Saleem³

et al. 2022

Aquaculture Research

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The present study was conducted to examine the potential effects of two dietary supplements, the probiotic, Lactobacillus fermentum (LB) (1 × 108 CFU/ml) and 0.5% cinnamon powder (CP) alone and in combination form [LB (1 × 108 CFU/ml) + CP (0.5%)] on growth, intestinal microbiota, immune and antioxidant system and resistance to Yersinia ruckeri infection in the rainbow trout, Oncorhynchus mykiss under high rearing density. Fish were fed experimental diets [control (T1): (non‐supplemented fish), T2: fish fed 1 × 108 CFU/ml L. fermentum, T3: fish fed 0.5% CP and T4: fish fed 1 × 108 CFU/ml L. fermentum + 0.5% CP] for 60 days. After the feeding period, the growth performance, intestinal microbiota, immunity and antioxidant defence were assayed and fish challenged with Y. ruckeri. The supplements alone or in combination showed higher final weight, weight gain and lower feed conversion ratio compared with control. However, the specific growth rate and protein efficiency ratio improved when the supplements were used in combination. The supplementation of fish with L. fermentum alone and in combination with CP increased the activity of protease and amylase (p < 0.05). Although the supplementation of fish with the L. fermentum alone or in combination with CP enhanced immune and antioxidant defence components in serum and mucus, this effect was more pronounced when fish fed L. fermentum + CP. The L. fermentum alone or in combination with CP reduced the fish stress, as the concentrations of glucose and cortisol decreased in these treatments. Alkaline phosphatase activity in blood in fish fed CP and those fed L. fermentum + CP and lactate dehydrogenase activity in fish fed L. fermentum + CP decreased compared with control. Furthermore, fish supplemented with L. fermentum + CP significantly increased resistance to Y. ruckeri infection (p < 0.05). In conclusion, the findings of the present study indicated that a combination of CP and L. fermentum in the diet can be more effective in improving growth, immunity, digestive enzyme performance and resistance to Y. ruckeri infection in rainbow trout, suggesting a synergistic effect for them.

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“…It has been reported that A . hydrophila infection in common carp Cyprinus carpio can enhance the GOT and GPT activity in the serum (Krishnaveni et al, 2021), indicating that A . hydrophila infection brings damage to fish liver.…”

Section: Discussionmentioning

confidence: 99%

“…Cyprinus carpio can enhance the GOT and GPT activity in the serum (Krishnaveni et al, 2021), indicating that A. hydrophila infection brings damage to fish liver. While in this study, only GPT activity in the serum was significantly increased in LG and MG of WM-1+KS02 groups, and the enhancement of GPT activity was suppressed to the normal level in HG of WM-1+KS02 group.…”

Section: It Has Been Reported That a Hydrophila Infection In Common Carpmentioning

confidence: 99%

Bacillus methylotrophicus WM‐1 enhances the immunity of grass carp against Aeromonas hydrophila

Li

¹

,

Luo

²

,

Yang

³

et al. 2022

Aquaculture Research

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Aeromonas hydrophila is a common pathogen and causes huge economic losses in aquaculture; to date, we still have no efficient methods to control fish diseases caused by A. hydrophila. Our previous research found that a strain of Bacillus methylotrophicus WM-1 could effectively inhibit A. hydrophila KS02. In the current study, the safety of WM-1 and its protective effects against A. hydrophila in the aquaculture system were examined. Grass carp were selected and fed with WM-1 supplemented feed (0, 10 3 , 10 5 and 10 7 CFU/g feed, as CG, LG, MG and HG) for a period of 90 days. On day 30, grass carp were challenged with KS02 (WM-1+KS02 group) or saline (WM-1 group) as control. The growth performance and enzyme activities were measured; blood routine tests were conducted on day 90. In the WM-1 group, AKP activity in the serum was significantly reduced and SOD activity in the liver was significantly enhanced by WM-1 supplemented feed, while other monitored indices had no significant changes. In the WM-1+KS02 group, GPT activity in the serum and SOD activity in the liver were significantly enhanced, while AKP activity in the serum had no significant change except for the MG group. Survival rates of grass carp were advanced by WM-1 supplemented feed, reaching 70% after challenge with KS02. Overall, WM-1 could maintain the homeostasis of grass carp and protected them against A. hydrophila infection efficiently, which indicated WM-1 would be a probiotic candidate for use in aquaculture.

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Effects of dietary supplementation of Lactobacillus fermentum URLP18 on growth, innate immunity and survival against Aeromonas hydrophila ATCC 7966 challenge in freshwater fish Cyprinus carpio (common carp)

Cited by 16 publications

References 76 publications

Effects of Dietary Supplementation of PrimaLac, Inulin, and Biomin Imbo on Growth Performance, Antioxidant, and Innate Immune Responses of Common Carp (Cyprinus carpio)

Effects of Dietary Supplementation of PrimaLac, Inulin, and Biomin Imbo on Growth Performance, Antioxidant, and Innate Immune Responses of Common Carp (Cyprinus carpio)

The synergistic effects of the probiotic ( Lactobacillus fermentum ) and cinnamon, Cinnamomum sp. powder on growth performance, intestinal microbiota, immunity, antioxidant defence and resistance to Yersinia ruckeri infection in the rainbow trout ( Oncorhynchus mykiss ) under high rearing density

Bacillus methylotrophicus WM‐1 enhances the immunity of grass carp against Aeromonas hydrophila

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