Effects of dietary supplementation with Pediococcus acidilactici ZPA017 on reproductive performance, fecal microbial flora and serum indices in sows during late gestation and lactation

Abstract

Objective: This study was conducted to determine the effects of dietary supplementation with Pediococcus acidilactici (P. acidilactici) ZPA017 as a probiotic on reproductive perfor mance, fecal microbial flora and serum indices in sows during late gestation and lactation. Methods: A total of 94 sows (Large White×Yorkshire, average 4.50 parities) were randomly allotted to two dietary treatments: control diet and the diet supplemented with P. acidilactici ZPA017 (2.40×10 9 colonyforming unit/kg of diets). The study started at d 90 of gestation and conducted until d 28 of lactation. Results: Compared to sows fed the control diet, supplementation of P. acidilactici ZPA017 increased the number of weaning piglets, weaning weight of litter and piglets, survival rate of piglets at weaning (p<0.05), and decreased diarrhea rate of piglets in lactation (p<0.05). Dietary P. acidilactici ZPA017 increased fecal Lactobacillus populations (p = 0.030) and reduced fecal Escherichia coli and Staphylococcus aureus populations (p<0.05) of sows at weaning. Moreover, the supplementation of P. acidilactici ZPA017 increased serum concentrations of immunoglobulin G, immunoglobulin A and total protein (p<0.05), while decreased serum haptoglobin concentration and alanine aminotransferase activity (p<0.05) of sows at weaning. Conclusion: Administration of P. acidilactici ZPA017 in diets during late gestation and lac tation had positive effects on the reproductive performance, intestinal microflora balance and immunity of sows.

Bacillus subtilis QST 713 Supplementation during Late Gestation in Gilts Reduces Stillbirth and Increases Piglet Birth Weight

“…The increased NBA in the treatment group was in agreement with some previous findings [ 5 , 8 , 9 ]. However, many other studies reported nonsignificant effect of probiotic supplementation on this criterion [ 4 , 6 , 7 , 10 , 14 – 20 ]. The positive effect of the probiotic supplementation on NBA may be partly attributable to the fact that it reduced stillbirth rate.…”

“…It is interesting that Bacillus subtilis QST 713 increased both litter size/NBA and birth weight despite the fact that birth weight is negatively associated with litter size [ 25 , 26 ]. Some previous studies failed to find any beneficial effects of probiotic supplementation at late gestation on birth weight of piglets [ 7 – 9 , 20 ] while other studies even found a reduced birth weight in the probiotic supplementation group where litter and/or NBA increased in comparison with that in the control [ 5 , 14 ]. Two previous studies found that birth weight was increased while litter sizes were unaltered when probiotic was supplemented throughout 2 successive estrus cycles [ 27 ] or late gestation [ 28 ].…”

Bacteriocinogenic lactic acid bacteria in the traditional cereal-based beverage Boza: a genomic and functional approach

“…Antibiotic resistance, prophages, plasmid and plasmid-associated bacteriocins have been indicated as source of variability at P. pentosaceus genomes 9 . Our P. acidilactici strain, however, also contains a plasmidassociated bacteriocin operon (pediocin PA-1), differing from other strains reported in the literature, such as ZPA017 and BCC-1 10,11 . The metabolic characteristics of the strains obtained indicated a wider metabolic potential for carbohydrate transport for the P. acidilactii transport while the P. pentosaceus presented he capability to synthesize lysine and had quorum sensing related genes.…”

“…Genome analysis indicated that these three isolates likely belong to two different Pediococcus species: one P. acidilactii, and two P. pentosaceus. The P. acidilactici ST31BZ genome showed similar values of size, GC content, number of predicted proteins and ribosomal operons as other P. acidilactici strains 10,12 . Detected CRISPR systems are similar to previously described in P. acidilactici HN9 13 .…”

“…Based on the pangenomes of our isolates against reference genomes, we propose P. pentosaceus ST31BZ as a new strain of P. acidilactici and strains ST75BZ and ST87BZ as a new strain of P. pentosaceus. The two closest strains to P. acidilactici were ZPA017 and BCC-1, isolated from feces of a healthy pig 10 and cecum of a broiler chicken 11 , respectively. The unique genes present in our isolates were related to antibiotic resistance and prophages, such as glycopeptide antibiotics resistance protein (COG4767) and phage portal protein BeeE (COG4695) in P. acidilactici ST31BZ (Supplementary Table 3); and ABC-type multidrug transport system (COG0842) and phage terminase large subunit (COG1783) in P. pentosaceus ST75BZ and ST87BZ.…”