Effects of dietary supplementation with 17β-estradiol and 17α-methyltestosterone on growth performance and gonadal development of the juvenile oriental river prawn (Macrobrachium nipponense)

Jin, Shubo; Yue, Da; Fu, Hongtuo; Jiang, Sufei; Xiong, Yiwei; Qiao, Hui; Zhang, Wenyi; Gong, Yongsheng; Wu, Yan

doi:10.1016/j.aqrep.2022.101042

Cited by 7 publications

(6 citation statements)

References 36 publications

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“…Previous studies reported that gonad development has negative effects on the growth performance in aquatic species, which is consistent with these results 42,43 . The treatment dose of E2 was significantly higher in the M. nipponense study than in studies of vertebrate species, which illustrates differences between crustaceans and vertebrate species in response to steroid hormones 41 …”

Section: The Major Regulatory Factors Influences Male Sex Differentia...supporting

confidence: 89%

“…Previous studies reported that E2 has dramatic regulatory effects on growth performance 35–37 and testis development in many aquatic species 38–40 . Jin et al 41 provided five different concentrations of E2 (0, 100, 250, 500 and 1000 mg/kg) in the diet of male juvenile M. nipponense , and the treated dose from 500 mg/kg promoted growth and inhibited testis development by inhibiting the expression of sperm gelatinase (Mn‐SG) and insulin‐like androgenic gland (Mn‐IAG) and the secretion of testosterone. Previous studies reported that gonad development has negative effects on the growth performance in aquatic species, which is consistent with these results 42,43 .…”

Section: The Major Regulatory Factors Influences Male Sex Differentia...mentioning

confidence: 99%

“…42,43 The treatment dose of E2 was significantly higher in the M. nipponense study than in studies of vertebrate species, which illustrates differences between crustaceans and vertebrate species in response to steroid hormones. 41…”

Section: -Estradiol (E2)mentioning

confidence: 99%

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Recent progress of male sexual differentiation and development in the oriental river prawn (Macrobrachium nipponense): A review

Jin

Zhang

Xiong

et al. 2022

Reviews in Aquaculture

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Many researchers have focused on male sexual differentiation and development of the Macrobrachium nipponense in recent years. In this review, we highlight the status of knowledge about male sexual differentiation and development in this species by summarizing the chromosome analyses, major regulatory factors, identification of male reproduction-related genes and gene functional analyses. Fifty-two chromosomes have been identified in the testis of M. nipponense, which led to the identification of sex-related chromosomes in this prawn. Studies have shown that hormones secreted by the eyestalk, androgenic gland, and testis as well as 17-estradiol and environmental factors regulate the process of male sexual development in M. nipponense. The sex differentiation and development sensitive period was identified as post-larva 10 (PL10) to PL22; therefore, this time span is critical in the identification of male reproduction-related genes from M. nipponense. In this study, 80 male sex-related homologous genes were identified based on functional analysis in other species, and another 45 genes were considered to be strong male reproductionrelated candidates based on their expression changes through performing multiomics profiling analyses. Quantitative polymerase chain reaction analysis revealed 25 genes that were involved in the process of male sexual development in M. nipponense, and RNA interference showed that knockdown of the expression levels of SDHB, PDHE1, NFkBa, HSDL1 and ferritin delayed testis development. Use of Crispr-Cas9 technology and analysis of noncoding RNA should be further investigated, which will be effective in establishing an artificial technique to regulate the process of male sexual development in M. nipponense.

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Section: The Major Regulatory Factors Influences Male Sex Differentia...supporting

confidence: 89%

Section: The Major Regulatory Factors Influences Male Sex Differentia...mentioning

confidence: 99%

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Recent progress of male sexual differentiation and development in the oriental river prawn (Macrobrachium nipponense): A review

Jin

Zhang

Xiong

et al. 2022

Reviews in Aquaculture

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“…In studies on grass carp ( C. idella) , it has been proposed that this phenomenon occurs because MT inhibits ovarian development and the energy used for ovarian development is used for growth and development [ 49 ]. Furthermore, a previous study on the oriental river prawn revealed that dietary supplementation with MT at concentrations greater than 500 mg/kg delayed ovarian development in juvenile prawns [ 50 ]. In this study, the WGR and SGR of female prawns in the 100 and 200 mg/kg MT were significantly increased compared to those of the control group.…”

Section: Discussionmentioning

confidence: 99%

Sex Reversal Induced by Dietary Supplementation with 17α-Methyltestosterone during the Critical Period of Sex Differentiation in Oriental River Prawn (Macrobrachium nipponense)

Cai

Yuan

Gao

et al. 2023

Animals

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The steroid 17α-methyltestosterone (MT) inhibits ovarian function and is often used to induce sex reversal artificially in vertebrates. In the present study, different concentrations of MT were added as dietary supplementation, and the effects on sex ratio, growth, and gonadal development were examined. After 40 days, the sex ratio (male:female) in each group increased at different degrees with 50 (1.36:1), 100 (1.57:1), and 200 (2.61:1) mg/kg MT, and neo-males with testis–ovary coexistence were observed in the 200 mg/kg MT group. Furthermore, 50 and 100 mg/kg MT could induce female reversion in neo-males. Histologically, the development of the testes in experimental groups was slower, but the ovaries of the experimental and control groups had similar developmental rates. The expression levels of DMRT11E, Foxl2, and SoxE1 in males at 200 mg/kg MT were 8.65-, 3.75-, and 3.45-fold greater than those of the control group. In crustaceans, sex reversal through vertebrate sex hormones can be observed. Neo-males (sex-reversed female prawns) were maintained by exogenous androgen, and over-reliance led to slow testis growth, small body size, and low growth rate, but sperm was still produced. In female prawns, MT inhibited ovary development and promoted growth.

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“…To address this issue, we used dsIAG to artificially control sex and develop monosex cultivation techniques. Previous studies have indicated that crustaceans possess sex plasticity, but it was a challenge to induce sex reversal when the developmentally sensitive period passed [ 22 , 23 ]. Sex formation in crustaceans involves sex determination and sex differentiation.…”

Section: Introductionmentioning

confidence: 99%

Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation

Cai,

Zhang,

Jiang

et al. 2023

IJMS

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This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium nipponense and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether dsIAG could induce sex reversal in PL30-male M. nipponense during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM). After six injections, the experiment finally resulted in a 20% production of dsRM. Histologically, dsRM ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A total of 1718, 1069, and 255 differentially expressed genes were identified through transcriptome sequencing of the gonads in three comparison groups, revealing crucial genes related to reproduction and sex differentiation, such as GnRHR, VGR, SG, and LWS. Principal Component Analysis (PCA) also distinguished dsM and dsRM very well. In addition, this study predicted that the eyestalks and the “phototransduction-fly” photoperiodic pathways of M. nipponense could play an important role in sex reversal. The enrichment of related pathways and growth traits in dsNRM were combined to establish that IAG played a significant role in reproduction, growth regulation, and metabolism. Finally, complete sex reversal may depend on specific stimuli at critical periods. Overall, this study provides valuable findings for the IAG regulation of sex differentiation, reproduction, and growth of M. nipponense in establishing a monoculture.

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Effects of dietary supplementation with 17β-estradiol and 17α-methyltestosterone on growth performance and gonadal development of the juvenile oriental river prawn (Macrobrachium nipponense)

Cited by 7 publications

References 36 publications

Recent progress of male sexual differentiation and development in the oriental river prawn (Macrobrachium nipponense): A review

Recent progress of male sexual differentiation and development in the oriental river prawn (Macrobrachium nipponense): A review

Sex Reversal Induced by Dietary Supplementation with 17α-Methyltestosterone during the Critical Period of Sex Differentiation in Oriental River Prawn (Macrobrachium nipponense)

Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation

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