Effects of dietary transgenic poplar leaf pellets on performance and tissues in rabbits

Yang, Liyan; Sun, Yuqiang; Wang, Yixue; Yaoshan, Hao

doi:10.1002/jsfa.6388

Cited by 7 publications

(1 citation statement)

References 20 publications

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“…It has been reported that United State livestock industry annually produced more than nine billions of livestock and over 95% of these animals are raised on GM based diets (Van Eenennaam & Young, ). Various studies have independently demonstrated the nutritional value and safe use of GM crops as a feed ingredients in livestock production (Cheng et al, ; García‐Villalba et al, ; Herman & Price, ; Hollingworth et al, ), and insignificant changes related to health, performance and feed digestibility were recorded (Flachowsky, Schafft, & Meyer, ; Mao et al, ; Rahman, Zaman, Shaheen, Irem, & Zafar, ; Salisu, Shahid, Ali, Rao, & Husnain, ; Shahid et al, ; Yang, Sun, Hao, & Wang, ; Yang, Sun, Wang, & Hao, ; Yuan, Tang, Zhao, Shi, & Wang, ). Studies have also not yet reported significant variations relating to any alterations in the nutrients components of animal products obtained from livestock consuming GM‐based feed (Van Eenennaam & Young, ).…”

Section: Introductionmentioning

confidence: 99%

Assessing the fate of recombinant plant DNA in rabbit’s tissues fed genetically modified cotton

Shahid

Salisu

Yaqoob

et al. 2019

Animal Physiology Nutrition

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| INTRODUC TI ONOver the past few years, transgenic crops have been considered as diet source for both animal and humans. Recently, these crops are significantly becoming part of the feed ingredients in livestock industries (Świątkiewicz et al., 2010). Food-producing animals globally consumed over 70% of genetically modified (GM) crops biomass. It has been reported that United State livestock industry annually produced more than nine billions of livestock and over 95% of these animals are raised on GM based diets (Van Eenennaam & Young, 2014b). Various studies have independently demonstrated the nutritional value and safe use of GM crops as a feed ingredients in livestock production (Cheng et al. AbstractVarious feeding studies have been conducted with the different species of animals to evaluate the possible transfer of transgenic DNA (tDNA) from genetically modified (GM) feed into the animal tissues. However, the conclusions drawn from most of such studies are sometimes controversial. Thus, in the present study, an attempt has been made to evaluate the fate of tDNA in rabbits raised on GM cotton-based diet through PCR analysis of the DNA extracted specifically from blood, liver, kidney, heart and intestine (jejunum). A total of 48 rabbits were fed a mixed diet consisting variable proportions of transgenic cottonseeds meal (i.e. 0% w/w, 20% w/w, 30% w/w and 40% w/w) for 180 days. The presence of transgenic DNA fragments (Cry1Ac, Cry2A and CP4 EPSPS) or plant endogenous gene (Sad1) was traced in those specific tissues and organs. The presence of β-actin (ACTB) was also monitored as an internal control. Neither the transgenic fragments (459 bp of Cry1Ac gene, 167 bp of Cry2A gene and111 bp of CP4 EPSPS gene) nor cotton endogenous reference gene (155 bp of Sad1) could be detected in any of the DNA samples extracted from the rabbit's tissues in both control and transgenic groups. However, 155 bp fragment of the rabbit's reference gene (ACTB) was recovered in all the DNA samples extracted from rabbit tissues. The results obtained from this study revealed that both plant endogenous and transgenic DNA fragments have same fate in rabbit's tissues and were efficiently degraded in the gastrointestinal tract (GIT). K E Y W O R D S fate of tDNA,

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