Effects of dietary vegetable oils on the expression of eicosanoid receptors genes in Senegalese sole (Solea senegalensis) intestine

Montero, Daniel; Torrecillas, Silvia; Benitez-Dorta, Vanessa; Caballero, M. J.; Izquierdo, M.S.; Zamorano, M. J.

doi:10.1016/j.aqrep.2019.100201

Cited by 10 publications

(11 citation statements)

References 44 publications

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“…The reports on the effect of dietary n-6 FA levels on the fish immune system are contradictory, ranging from no apparent dietary effects (Andresen et al, 2019;Bransden, Carter & Nichols, 2003;Gjøen et al, 2005), as seen in the present study, to reduced resistance against or increased mortality from pathogens (Thompson, Tatner & Henderson, 1996;Martinez-Rubio et al, 2012;Estensoro et al, 2012;Montero et al, 2008;Montero et al, 2010;Montero et al, 2019). Furthermore, it has been suggested that other dietary nutrients such as the sources of protein, availability of micronutrients, species differences, and experimental conditions may all impact the immune response.…”

Section: Discussioncontrasting

confidence: 68%

Impact of dietary level and ratio of n-6 and n-3 fatty acids on disease progression and mRNA expression of immune and inflammatory markers in Atlantic salmon (Salmo salar) challenged with Paramoeba perurans

Selvam

Powell

Liland

et al. 2021

PeerJ

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The aim of the study was to investigate the influence of dietary level and ratio of n-6/n-3 fatty acids (FA) on growth, disease progression and expression of immune and inflammatory markers in Atlantic salmon (Salmo salar) following challenge with Paramoeba perurans. Fish (80 g) were fed four different diets with different ratios of n-6/n-3 FA; at 1.3, 2.4 and 6.0 and one diet with ratio of 1.3 combined with a higher level of n-3 FA and n-6 FA. The diet with the n-6/n-3 FA ratio of 6.0 was included to ensure potential n-6 FA effects were revealed, while the three other diets were more commercially relevant n-6/n-3 FA ratios and levels. After a pre-feeding period of 3 months, fish from each diet regime were challenged with a standardized laboratory challenge using a clonal culture of P. perurans at the concentration of 1,000 cells L−1. The subsequent development of the disease was monitored (by gross gill score), and sampling conducted before challenge and at weekly sampling points for 5 weeks post-challenge. Challenge with P. perurans did not have a significant impact on the growth of the fish during the challenge period, but fish given the feed with the highest n-6/n-3 FA ratio had reduced growth compared to the other groups. Total gill score for all surfaces showed a significant increase with time, reaching a maximum at 21 days post-challenge and declined thereafter, irrespective of diet groups. Challenge with P. perurans influenced the mRNA expression of examined genes involved in immune and inflammatory response (TNF-α, iNOS, IL4-13b, GATA-3, IL-1β, p53, COX2 and PGE2-EP4), but diet did not influence the gene expression. In conclusion, an increase in dietary n-6/n-3 FA ratio influenced the growth of Atlantic salmon challenged with P. perurans; however, it did not alter the mRNA expression of immune genes or progression of the disease.

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Section: Discussioncontrasting

confidence: 68%

Impact of dietary level and ratio of n-6 and n-3 fatty acids on disease progression and mRNA expression of immune and inflammatory markers in Atlantic salmon (Salmo salar) challenged with Paramoeba perurans

Selvam

Powell

Liland

et al. 2021

PeerJ

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“…However, after the inflammatory insult with killed Phdp the expression of these genes was modulated by the diets, with an overall higher expression in fish fed diet C (0:0.6:0.6; ARA:EPA:DHA) and D (0:0.3:1.5; ARA:EPA:DHA). Different dietary effects on immune-related genes before and after a stressful or bacterial challenge were already reported in different fish species (Montero et al, 2019;Rivero-Ramirez et al, 2020).…”

Section: Discussionmentioning

confidence: 86%

“…In fact, after a bacterial challenge with V.anguillarum in European seabass fed graded levels of ARA (0.5% to 6% FA) the expression of cox 2 and il‐1β in the DI was up‐regulated only in diets with 0.5% and 2% ARA of total FA showing that the pro‐inflammatory effect of ARA is not progressively correlated with the dietary content of this EFA (Rivero‐Ramirez et al, 2020). In Senegalese sole ( Solea senegalensis ), submitted to a chasing‐stress challenge, expression of EP 4 (low affinity prostaglandin E 2 receptor) increased in fish fed the FO and linseed oil diets and the expression of EP 2 (high affinity prostaglandin E 2 receptor) and phospholipase A 2 gene decreased in fish fed linseed oil diet, while a soybean oil diet did not modulate this gene expression, compared to the non‐stress conditions (Montero et al, 2019). These results suggested that fish fed FO and linseed oil‐based diets, with higher levels of n ‐3 PUFA than soybean oil, were better prepared to deal with a stressful situation than those fed soybean oil diet (high n ‐6 PUFA content).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effect of different dietary ARA/EPA/DHA ratios in gilthead sea bream ( Sparus aurata ) juveniles after infection with Photobacterium damselae subsp. piscicida

Magalhães

Guardiola

Guerreiro

et al. 2022

Aquaculture Research

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The immunomodulatory effect of different dietary arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) ratios was evaluated in gilthead sea bream juveniles after intraperitoneal injection (i.p.) with formalin‐killed Photobacterium damselae subsp. piscicida (Phdp). Fish were fed with four isoproteic (47% crude protein) and isolipidic (18% crude lipids) diets with the following long‐chain polyunsaturated fatty acids (LC‐PUFA) ratios: Diet A (2.0% ARA: 0.2% EPA: 0.1% DHA); Diet B (1.0% ARA: 0.4% EPA: 0.4% DHA); Diet C (0% ARA: 0.6% EPA: 0.6% DHA); Diet D (0% ARA: 0.3% EPA: 1.5% DHA). After being fed for 56 days with the experimental diets, fish from each dietary treatment were randomly selected and i.p. injected with 0.2 ml of killed Phdp (1 × 108 CFU ml−1) or phosphate‐buffered saline (control group). Four and twenty‐four hours (i.e., twice) after the i.p., the haematological profile, plasma humoral innate immune parameters, and the intestine expression of immune‐related genes were assessed. Results showed that fish fed diet D (high DHA content) had increased complement activity and higher intestinal expression of cyclooxygenase 2 (cox2) and interleukin 1β (il‐1β) at 4 and 24 hours after i.p., as well as increased intestinal tumour necrosis factor α (tnfα) expression at 4 hours. On the other hand, fish fed with diet A (high ARA content) showed higher plasma bactericidal activity against Phdp than fish fed with diet D. Overall, the results of this study suggest a positive modulatory effect of DHA in response to the pathogenic agent.

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“…Long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic (EPA,20:5ω3), docosahexaenoic (DHA,22:6ω3) and arachidonic (ARA, 20:4ω6) acids play important roles in fish growth, metabolism (Sargent et al, 2002), neural development, health and reproduction (Sargent et al, 1999;Tocher, 2010Tocher, , 2015. They are also important structural components of cell membranes (Calder, 2013), and act as precursors to eicosanoid metabolites such as prostaglandins, leukotrienes, thromboxanes, docosanoids, maresins and resolvins which regulate inflammatory and immune response processes (Calder, 2007(Calder, , 2013Arts and Kohler, 2009;Martinez-Rubio et al, 2013;Holen et al, 2015;Montero et al, 2019). The levels of EPA and DHA in fish feeds showed a large reduction (from ∼ 20 to 5% of dietary lipid) over the past ∼3 decades (Einen and Thomassen, 1998;Ytrestøyl et al, 2015;Sprague et al, 2016), due to decreasing global availability and rising market price of their fish oil (FO) sources (Turchini et al, 2009;Tacon and Metian, 2015;Froehlich et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Influence of Dietary Long-Chain Polyunsaturated Fatty Acids and ω6 to ω3 Ratios on Head Kidney Lipid Composition and Expression of Fatty Acid and Eicosanoid Metabolism Genes in Atlantic Salmon (Salmo salar)

Katan

Xue

Caballero‐Solares

et al. 2020

Front. Mol. Biosci.

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The interaction of dietary eicosapentaenoic acid and docosahexaenoic acid (EPA+DHA) levels with omega-6 to omega-3 ratios (ω6:ω3), and their impact on head kidney lipid metabolism in farmed fish, are not fully elucidated. We investigated the influence of five plant-based diets (12-week exposure) with varying EPA+DHA levels (0.3, 1.0, or 1.4%) and ω6:ω3 (high ω6, high ω3, or balanced) on tissue lipid composition, and transcript expression of genes involved in fatty acid and eicosanoid metabolism in Atlantic salmon head kidney. Tissue fatty acid composition was reflective of the diet with respect to C18 PUFA and MUFA levels (% of total FA), and ω6:ω3 (0.5–1.5). Fish fed 0.3% EPA+DHA with high ω6 (0.3% EPA+DHA↑ω6) had the highest increase in proportions (1.7–2.3-fold) and in concentrations (1.4-1.8-fold) of arachidonic acid (ARA). EPA showed the greatest decrease in proportion and in concentration (by ~½) in the 0.3% EPA+DHA↑ω6 fed fish compared to the other treatments. However, no differences were observed in EPA proportions among salmon fed the high ω3 (0.3 and 1.0% EPA+DHA) and balanced (1.4% EPA+DHA) diets, and DHA proportions were similar among all treatments. Further, the transcript expression of elovl5a was lowest in the 0.3% EPA+DHA↑ω6 fed fish, and correlated positively with 20:3ω3, 20:4ω3 and EPA:ARA in the head kidney. This indicates that high dietary 18:3ω3 promoted the synthesis of ω3 LC-PUFA. Dietary EPA+DHA levels had a positive impact on elovl5a, fadsd5 and srebp1 expression, and these transcripts positively correlated with tissue ΣMUFA. This supported the hypothesis that LC-PUFA synthesis is positively influenced by tissue MUFA levels in Atlantic salmon. The expression of pparaa was higher in the 0.3% EPA+DHA↑ω6 compared to the 0.3% EPA+DHA↑ω3 fed fish. Finally, significant correlations between head kidney fatty acid composition and the expression of eicosanoid synthesis-related transcripts (i.e., 5loxa, 5loxb, cox1, cox2, ptges2, ptges3, and pgds) illustrated the constitutive relationships among fatty acids and eicosanoid metabolism in salmon.

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Effects of dietary vegetable oils on the expression of eicosanoid receptors genes in Senegalese sole (Solea senegalensis) intestine

Cited by 10 publications

References 44 publications

Impact of dietary level and ratio of n-6 and n-3 fatty acids on disease progression and mRNA expression of immune and inflammatory markers in Atlantic salmon (Salmo salar) challenged with Paramoeba perurans

Impact of dietary level and ratio of n-6 and n-3 fatty acids on disease progression and mRNA expression of immune and inflammatory markers in Atlantic salmon (Salmo salar) challenged with Paramoeba perurans

Immunomodulatory effect of different dietary ARA/EPA/DHA ratios in gilthead sea bream ( Sparus aurata ) juveniles after infection with Photobacterium damselae subsp. piscicida

Influence of Dietary Long-Chain Polyunsaturated Fatty Acids and ω6 to ω3 Ratios on Head Kidney Lipid Composition and Expression of Fatty Acid and Eicosanoid Metabolism Genes in Atlantic Salmon (Salmo salar)

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