Effects of diets supplemented with either individual or combined probiotics,Bacillus subtilisE20 andLactobacillus plantarum7-40, on the immune response and disease resistance of the mud crab,Scylla paramamosain(Estampador)

Abstract

Effects of diets supplemented with either individual or combined probiotics, Bacillus subtilis E20 and Lactobacillus plantarum 7-40, on the immune response and disease resistance of the mud crab, Scylla paramamosain (Estampador) Abstract Two trials, including firstly, diets incorporating individual or combined species of probiotics, Bacillus subtilis E20 and Lactobacillus plantarum 7-40, were fed to the mud crab, Scylla paramamosian, for 28 days to evaluate the growth, immunity and disease resistance, and secondly, the optimal level of B. subtilis E20 in the diet by tracking the growth, immunity and disease resistance after 28 days of feeding were analysed. No significant differences in growth, total haemocyte counts, respiratory bursts, superoxide dismutase, or glutathione peroxidase were found in the two trials. Crabs fed the B. subtilis E20-containing diet (D20) had significantly increased phenoloxidase (PO) activity, phagocytic activity (PA) and disease resistance compared with crabs fed the control and the mixed probiotics diet (MD). The mortality of crabs fed the D20 was significantly lower than that of crabs fed the L. plantarum 7-40-containing diet. L. plantarum 7-40 had a great inhibitory effect on the growth of B. subtilis E20, which may have led to the decreased probiotic effect of the MD. An analysis of the optimal level of B. subtilis E20 in the diet showed that crabs fed the B. subtilis E20-containing diet at a level of 10 9-10 cfu kg À1 had better immune response of PO and PA, and disease resistance in the second trial.

The effects of dietary Bacillus subtilis on immune response, hematological parameters, growth performance, and resistance of juvenile olive flounder (Paralichthys olivaceus) against Streptococcus iniae

“…The improved growth performance caused by BS administration was also reported in shrimp (Sadat Hoseini Madani, Adorian, Ghafari Farsani, & Hoseinifar, ) and sea cucumber ( Apostichopus japonicas ; Zhao et al, ). However, the administration of BS in large yellow croaker and mud crab ( Scylla paramamosain ) diets showed no remarkable effects on growth performance (Ai et al, ; Yeh, Chiu, Shiu, Huang, & Liu, ). The growth‐enhancing effect of BS in this study was mainly because of an improved feed utilization efficiency shown in FCR and PER, which is in agreement with many previous studies (Ai et al, ; Bagheri, Hedayati, Yavari, Alizade, & Farzanfar, ).…”

Effects of synbiotic containing Lactobacillus plantarum 7-40 and galactooligosaccharide on the growth performance of white shrimp, Litopenaeus vannamei

“…plantarum 7–40 were examined for ability to induce bacterial enzyme activities, including protease, leu‐aminopeptidase and β‐galactosidase. In addition, the selected prebiotics were also investigated for supporting the growth of pathogenic bacteria, including V. alginolyticus (Liu, Cheng, Hsu, & Chen, ) and V. parahaemolyticus (Yeh, Chiu, Shiu, Huang, & Liu, ), as references. GOS, which was able to induce higher production of enzyme activity of a probiotic and exhibited significantly lower support for the growth of pathogenic bacteria, was used to formulate a synbiotic with Lac.…”

“…plantarum 7-40 were examined for ability to induce bacterial enzyme activities, including protease, leu-aminopeptidase and b-galactosidase. In addition, the selected prebiotics were also investigated for supporting the growth of pathogenic bacteria, including V. alginolyticus (Liu, Cheng, Hsu, & Chen, 2004) and V. parahaemolyticus (Yeh, Chiu, Shiu, Huang, & Liu, 2014), as references.…”

Antibiofilm potential of a tropical marineBacillus licheniformisisolate: role in disruption of aquaculture associated biofilms

“…There are a few reports on some compounds derived from Bacillus species inhibiting bacterial biofilms in general (Rivardo et al 2009;Nithya, Devi & Pandian 2011). The members of this genus have also been used for improving the health of aqua cultured species such as Fenneropenaeus indicus, Penaeus vannamei, Macrobrachium rosenbergii, Crassostrea virginica, Ctenopharyngodon idellus and Scylla paramamosain (Ziaei-Nejad, Rezaei, Takami, Lovett, Mirvaghefi & Shakouri 2006;Zhou, Wang & Li 2009;Rahiman, Jesmi, Thomas & Hatha 2010;Karim, Zhao, Rowley, Nelson & Gomez-Chiarri 2013;Zhang, Fu, Deng, Liang, Zheng, Sun, Zhu, Peng, Wang, Wenying & Li 2013;Yeh, Chiu, Shiu, Huang & Liu 2014). In the current investigation, we have shown the effectiveness of the CFS derived from B. licheniformis D1 in controlling and disrupting aquaculture associated pathogenic bacteria.…”