Effects of different amino acids on somatic embryogenesis of strawberry (Fragaria × ananassa Duch.)

Gerdakaneh, Mohammad; Mozafari, Ali-Akbar; Sioseh-Mardah, Adel; Sarabi, Behrooz

doi:10.1007/s11738-011-0725-9

Cited by 36 publications

(27 citation statements)

References 27 publications

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“…1). Similarly, several reports confirmed that formation of SEs was enhanced by glutamine (Baskaran and Jayabalan 2009;Deo et al 2010;Gerdakaneh et al 2011). The role of picloram, TDZ and glutamine on SEs production has also been reported in other plant species (Tribulato et al 1997;Van Staden 2012, 2014).…”

Section: Resultssupporting

confidence: 53%

“…Furthermore, combination of picloram, TDZ and glutamine induced high production of SEs (Table 1). The addition of an organic nitrogen form has a positive influence on somatic embryogenesis (Robichaud et al 2004;Zouine and Hadrami 2007;Gerdakaneh et al 2011). Glutamine has been shown to improve somatic embryogenesis in both monocotyledons and dicotyledons (Dhir et al 1991;Yin et al 1993;Ke et al 1996;Vani and Reddy 1996;Shrivastava and Chawla 2001).…”

Section: Resultsmentioning

confidence: 99%

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Somatic embryogenesis of Pelargonium sidoides DC.

Kumar

Moyo

Staden

2015

Plant Cell Tiss Organ Cult

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Pelargonium sidoides is a high value medicinal plant endemic to South Africa and the Lesotho Highlands. Establishing an efficient regeneration system through somatic embryogenesis is necessary for its conservation. An efficient reproducible protocol for in vitro plant regeneration via somatic embryogenesis of Pelargonium sidoides, an important medicinal plant, is reported for the first time. Embryogenic callus was obtained on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg L -1 picloram, 0.5 mg L -1 thidiazuron and 20 mg L -1 glutamine. Different developmental stages of somatic embryos (SEs: globular embryos, heart shaped embryos, torpedo shaped embryos and cotyledon embryo with radicle) were directly obtained and further matured from embryogenic callus by subsequent subculture on the same medium. The highest frequency of somatic embryos (25.89 ± 1.50) was recovered after 6 weeks. Scanning electron microscopic (SEM) analysis revealed the presence of embryogenic cell clusters that formed cotyledonary embryos. Mature somatic embryos germinated and developed into plantlets after 4 weeks on half-strength MS medium. High plant regeneration frequency (94.4 %) was achieved on half-strength MS medium supplemented with 1.0 mg L -1 gibberellic acid. Rooted plantlets were successfully acclimatized in the greenhouse with a survival rate of 90 %. The protocol developed would be helpful in reducing stress on natural habitats, provide a system for germplasm conservation, regeneration of large numbers of high value clonal plants for commercial production, the isolation of bioactive compounds and genetic transformational studies.

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Section: Resultssupporting

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Somatic embryogenesis of Pelargonium sidoides DC.

Kumar

Moyo

Staden

2015

Plant Cell Tiss Organ Cult

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“…Thus, it is usually recommended to take explants from plants grown under controlled conditions such as a growth room or greenhouse or from buds that flush from dormant shoots stored indoors. 7,37 To evaluate the best media for the shoot formation, the juvenile leaf explants of the three cultivars (Festival, Sweet Charlie, and Florida) were cultured on MS medium with TDZ at concentrations of 1, 2, and 3 mg/l, individually. Cultures were incubated at dark for a week, then transferred to light for at least three weeks.…”

Section: Adventitious Shoots Formationmentioning

confidence: 99%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome, which makes the breeding of new cultivars difficult. simple and efficient methods for transformation and regeneration are required for cultivars improvement in strawberries. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., Festival, sweet charlie, and Florida via direct organogenesis using the in vitro juvenile leaves as explants. explants were collected after sub-culturing on a propagation medium composed of Ms supplemented with 0.5 mg/l Ba; 0.1 mg/l Ga3 and 0.1 mg/l IBa. To select the suitable organogenesis, the explants of the three cultivars were cultured on Ms medium supplemented with different concentrations of TDZ (1, 2, 3, and 4 mg/l), then incubated at a temperature of 22 °c ± 2. Medium containing 2 mg/l TDZ revealed the best regeneration efficiency with the three cultivars (72% for Festival and 73% for sweet charlie and Florida). after 4 weeks the produced shoots were cultured on Ms medium with different concentrations of Ba and Kin to enhance shoot elongation. Results showed that the medium containing 1.5 mg/l Ba and 0.5 mg/l Kin revealed highest elongation efficiency (88% and 94%) for Festival and sweet charlie, respectively. On the other hand, medium media containing 1.5 mg/l Ba and 0.1 mg/l Kin showed highest elongation efficiency (90%) in Florida. elongated shoots were successfully rooted on Ms medium containing 1.5 mg/l Naa. Furthermore, transformation of the two cultivars, Festival and sweet charlie, has been established via Agrobacterium strain LBa44404 containing the plasmid pIsV2678 with gus-intron and bar genes. Three days post-cocultivation, GUs activity was screened using the histochemical assay. The results showed 16% and 18% of the tested plant materials had changed into a blue color for Festival and sweet charlie, respectively. Out of 120 explants only 13 shoots were developed on bialaphos medium for each cultivar, representing 10.8% bialaphos-resistant strawberry shoot. The presence of both the genes bar and uid a was detected by PcR and Northern, giving a transformation efficiency of 5%.

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“…Similarly, in strawberry when sucrose concentration was raised from 30 to 90 g/l callus fresh weight was decreased (Gerdakaneh et al 2010). According to Gerdakaneh et al (2009), low sucrose concentration is much more effective than high levels on callus fresh weight as the rise of sucrose concentration decreases callus fresh weight and this capacity could be attributed to an osmotic effect. In CAB-6P, the increase in sucrose concentration from 15 to 30 g/l led to lower callus induction frequencies.…”

Section: Resultsmentioning

confidence: 99%