Effects of different antifungal treatments on artificial incubation of the astacid crayfish (Pacifastacus leniusculus Dana) eggs

Celada, J.D.; Carral, J.M.; Sáez‐Royuela, M.; Melendre, P.M.; Aguilera, A.

doi:10.1016/j.aquaculture.2004.06.003

Cited by 32 publications

(37 citation statements)

References 23 publications

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“…Many authors described and used AI of crayfish eggs under controlled conditions (MASON, 1977b;CUKERZIS et al, 1978;RHODES, 1981;CUKERZIS, 1988CUKERZIS, , 1989CARRAL et al, 1992CARRAL et al, , 2004MATTHEWS and REYNOLDS, 1995;JÄRVENPÄÄ and ILMARINEN, 1995;PÉREZ et al, 1998ab, 1999PÉREZ et al, 1998ab, , 2003CELADA et al, 2000CELADA et al, , 2001CELADA et al, , 2004 in order to improve survival rate.…”

Section: Introductionmentioning

confidence: 99%

“…First method of AI covers only a short period (several days) of the final embryo development and before eggs remain attached to the maternal pleopods for several months (CUKERZIS et al, 1988). Second type of AI takes several months and eggs are removed at earlier embryonic phases PÉREZ et al, 1998ab, 1999PÉREZ et al, 1998ab, , 2003CELADA et al, 2000CELADA et al, , 2001CELADA et al, , 2004). Short AI is less demanding for energy, time and space compared to long AI but incubated eggs are more dependent on maternal care and outdoor conditions, where hatching success is unwarranted for long time (CUKERZIS, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…Various facilities were used to carry out AI: Zuger jar (STREMPEL, 1973;KÖKSAL, 1988 andCUKERZIS, 1988); vertical incubator designed for salmonid eggs (MASON, 1977b); apparatuses based on a moving tray (JÄRVENPÄÄ and ILMARINEN, 1995); special flow incubators for crayfish in a semi-recirculation water system and in a flow-through system (RHODES, 1981;MATTHEWS and REYNOLDS, 1995;CARRAL et al, 2004;CELADA et al, 1988CELADA et al, , 2004PÉREZ et al 1998ab, 1999.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Egg Bath and Daily Removal of Dead Eggs on Hatching Success and Production of Stage 2 Juveniles During Artificial Incubation in Noble Crayfish (Astacus Astacus L.)

Policar

Kozák

Martín

2006

Bull. Fr. Pêche Piscic.

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The effects of egg bath (iodine-detergent preparation) and daily removal of dead eggs on hatching success and production of juveniles in stage 2 were investigated during a short (sixteen days) artificial incubation (AI) of noble crayfish eggs.At the beginning of AI, eggs were in phase XII (pulsating heart appearance) and were incubated in 18 polyethylene 1liter jars (100 eggs/jar, egg density 4.5 eggs.cm -2 ). Six different treatments were tested during AI:-C: control group without removal of dead eggs and egg bath; -R: daily removal of dead eggs without egg bath; -R-LB: daily removal of dead eggs, low frequency of egg bath (once every five days); -R-FB: daily removal of dead eggs, frequent egg bath (once every three days); -LB: without removal of dead eggs, with low frequency of egg bath; -FB: without removal of dead eggs, with frequent egg bath.Egg bath was performed by iodine-detergent preparation Jodisol (dose 2 ml.l -1 and exposition time 2 minutes).Results showed a better hatching rate after removing dead eggs rather than using the egg bath. Three treatments (R; R-LB; R-FB) showed significantly better survival rates in stage 1 (86.3 ± 5.4%) and 2 (84.2 ± 5.4%) than control (74.3 ± 0.9% and 73.3 ± 0.5%, respectively). Two treatments (LB, FB) showed no statistically different survival rate in stage 1 and 2 (82.5 ± 5.5 and 80.7 ± 5.3%, respectively) than the other treatments.After AI, juveniles from all treatments were reared under controlled conditions. At the end of rearing period, juveniles reached a survival rate of 64.1 ± 0.5% with mean body length of 22.0 ± 1.6 mm and mean body weight of 266.0 ± 50.9 mg. Negative effects of AI on growth and survival of juveniles were not evidenced. Key-words:Astacus astacus, artificial incubation, jar, juvenile production. EFFICACITÉ DU BAIN DES OEUFS ET DE L´ENLÈVEMENT JOURNALIER DES OEUFS MORTS SUR L´ÉCLOSION ET LA PRODUCTION DES JUVÉNILES AU STADE 2 PENDANT L´INCUBATION ARTIFICIELLE DE L´ÉCREVISSE À PATTES ROUGES (ASTACUS ASTACUS L.) RÉSUMÉL´efficacité du bain des oeufs dans une solution d´iode -Jodisol, solution à 2 ml.l-1 -et de l´enlèvement journalier des oeufs morts sur l´éclosion et la production des juvéniles au stade 2 a été observée au cours de la période courte (16 jours) de l´incubation artificielle (IA) d´ oeufs de l´écrevisse à pattes rouges.Au début de IA des oeufs étaient à la phase XII (apparition de pulsations du coeur) et leur incubation s´est déroulée dans 18 flacons polyéthyléniques d´un litre (100 oeufs/flacon, densité de 4,5 oeufs.cm -2 ). Six traitements différents ont été examinés pendant IA : C : groupe témoin; pas d´enlèvement des oeufs morts ; pas de bain des oeufs ; R : enlèvement journalier des oeufs morts ; pas de bain des oeufs ; R-LB : enlèvement journalier des oeufs morts ; fréquence de bain des oeufs : une fois tous les cinq jours ; R-FB : enlèvement journalier des oeufs morts ; fréquence de bain des oeufs : une fois tous les trois jours ; LB : pas d´enlèvement des oeufs morts, fréquence de bain des oeufs diminuée ; FB : pas ...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Egg Bath and Daily Removal of Dead Eggs on Hatching Success and Production of Stage 2 Juveniles During Artificial Incubation in Noble Crayfish (Astacus Astacus L.)

Policar

Kozák

Martín

2006

Bull. Fr. Pêche Piscic.

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“…Consequently, removal of dead eggs leads to a higher hatching rate and higher juvenile survival. A variety of antifungal agents (formaldehyde, malachite green, hydrogen peroxide, isopropyl alcohol, copper sulphate, potassium permanganate and iodine) have already been tested on astacid crayfish eggs, but proved inefficient for regular use due do toxic characteristics for humans and/or eggs (formaldehyde, potassium permanganate, malachite green, iodine) (Meinertz et al 1995;Celada et al 2004;Sáez-Royuela et al 2009) or showed no antifungal effects (isopropyl alcohol, copper sulphate, hydrogen peroxide) (Celada et al 2004;Melendre et al 2006). Formaldehyde showed good effectiveness at high concentrations (Sáez-Royuela et al 2009) but would not be an option due to its reported harmful reproductive and developmental toxicities, including chromosome and DNA damage (Duong et al 2011) especially under eight degrees Celsius.…”

Section: Introductionmentioning

confidence: 99%

Survival of Early Stripped Eggs of the Noble Crayfish, <i>Astacus astacu</i>, and Effects of Saline Solution During Artificial Incubation

Seemann

Lorkowski

Schiffer

et al. 2014

Freshwater Crayfish

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There is growing interest in using recirculating aquaculture systems (RAS) to produce juvenile noble crayfish, Astacus astacus (Linnaeus), a valuable and once plentiful food species in Europe, now a highly endangered species. The survival rates of early stripped eggs of A. astacus were compared across anti-fungal treatments in an artificial RAS incubation system based on a saline bath of approx. 20 -22 mS cm -1 or 15 -16 PSU over two different durations. Time from fertilisation to hatching was 82 days or 1191 degreedays. Low survival rates (live successfully hatched juveniles at end of experimental period) between 11.0 ± 9.5% and 26.6 ± 3.7% were obtained. Survival was significantly affected by the duration of egg bath within the saline solution with twice as high survival rates when eggs were saline-bathed once every two days for five minutes in comparison to eggs without treatment. Fungal rates were significantly affected by treating the eggs with a saline solution with infection rates decreasing from 3.7 ± 1.0% to 0.8 ± 0.5% in the ten minute saline bath treatment. Appropriately applied saline bathing can markedly improve survival of early-stripped noble crayfish eggs. However, further research is required to determine whether viable levels of hatching success can be obtained using early stripping and saline treatment.

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“…For many years malachite green has been the most effective fungicide used in aquaculture but since 1991 and 1997 is not permitted for aquaculture in USA (Schereier et al, 1996) and EU respectively, because of its teratogenic and carcinogenic effects (Andersen et al, 2006;Sapkota et al, 2008). After a series of trials testing chemicals used in other aquatic species, Celada et al (2004) and Melendre et al (2006) reported formaldehyde as the most effective antifungal. Although formaldehyde is approved for use in fish culture in USA and EU, there is concern about user safety because of its suspected carcinogenicity and its potential adverse effects on the aquatic environment (Arndt et al, 2001;Gieseker et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Antifungal treatments in artificial incubation of crayfish eggs (Pacifastacus leniusculus, Astacidae): Searching for alternatives to formaldehyde

Carral

González

Celada

et al. 2009

Knowl. Managt. Aquatic Ecosyst.

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Key-words:antifungal treatment, artificial incubation, astacid crayfishConsidering the concerns about the safety of the use of formaldehyde as antifungal agent, the effects of three alternative chemicals (potassium sorbate, copper hydroxide and magnesium chloride) were tested in the artificial incubation of signal crayfish (Pacifastacus leniusculus) eggs. Eight treatments were performed during 15 min every other day: formaldehyde at 3000 ppm (control), potassium sorbate at 5000 and 10 000 ppm, copper hydroxide at 40, 60, 80 and 200 ppm, and magnesium chloride at 10 000 ppm. Eggs were incubated in a flow through system at a density of 20 eggs·cm −2 . After 61 days of incubation, the highest efficiencies were obtained with 200 ppm of copper hydroxide (77.6% of survivors to stage 2) with no significant differences from the control (74.3%). Lower concentrations of copper hydroxide resulted in high egg mortality whereas potassium sorbate and magnesium chloride were ineffective to avoid fungal growth and total egg mortality took place. From the obtained results, copper hydroxide baths at 200 ppm could be considered as a good alternative to formaldehyde. fongique et une mortalité totale des oeufs intervenait. À partir des résultats obtenus, des bains d'hydroxyde de cuivre à 200 ppm peuvent être considérés comme une bonne alternative au formaldéhyde.

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Effects of different antifungal treatments on artificial incubation of the astacid crayfish (Pacifastacus leniusculus Dana) eggs

Cited by 32 publications

References 23 publications

Effects of Egg Bath and Daily Removal of Dead Eggs on Hatching Success and Production of Stage 2 Juveniles During Artificial Incubation in Noble Crayfish (Astacus Astacus L.)

Effects of Egg Bath and Daily Removal of Dead Eggs on Hatching Success and Production of Stage 2 Juveniles During Artificial Incubation in Noble Crayfish (Astacus Astacus L.)

Survival of Early Stripped Eggs of the Noble Crayfish, <i>Astacus astacu</i>, and Effects of Saline Solution During Artificial Incubation

Antifungal treatments in artificial incubation of crayfish eggs (Pacifastacus leniusculus, Astacidae): Searching for alternatives to formaldehyde

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