Effects of Different Antioxidant Enzymes on the Tracheal Epithelium Regeneration After Chemical Burn

Волкова, А. Г.; Шарапов, М. Г.; Равин, В. К.; Gordeeva, A. E.; Karaduleva, E. V.; Мубаракшина, Э. К.; Temnov, A. A.; Фесенко, Е. Е.; Новоселов, В. И.

doi:10.18093/0869-0189-2014-0-2-84-90

Cited by 8 publications

(3 citation statements)

References 8 publications

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“…It should be mentioned that we have demonstrated earlier a powerful cytoprotective effect of exogenous Prdx6, using models of chemical and thermal burns of the upper respiratory tract in rats, which was manifested in a significant preservation (by 50% higher compared to the control) of the ciliated epithelium of the trachea [ 50 ]. This can be explained not only by the elimination of a wide range of hydroperoxides in the damaged area, but also, obviously, by the ability of Prdx6 to be effectively reduced and re-engaged (due to aiPLA2) in the antioxidant defense of epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

The Role of Phospholipase Activity of Peroxiredoxin 6 in Its Transmembrane Transport and Protective Properties

Шарапов

Гончаров

Parfenyuk

et al. 2022

IJMS

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Peroxiredoxin 6 (Prdx6) is a multifunctional eukaryotic antioxidant enzyme. Mammalian Prdx6 possesses peroxidase activity against a wide range of organic and inorganic hydroperoxides, as well as exhibits phospholipase A2 (aiPLA2) activity, which plays an important role in the reduction of oxidized phospholipids and cell membrane remodeling. Exogenous Prdx6 has recently been shown to be able to penetrate inside the cell. We hypothesized that this entry may be due to the phospholipase activity of Prdx6. Experiments using exogenous Prdx6 in three cell lines (3T3, A549, RAW 264.7) demonstrated that it is the phospholipase activity that promotes its penetration into the cell. Overoxidation of Prdx6 led to a suppression of the peroxidase activity and a 3-to-4-fold growth of aiPLA2, which enhanced the efficiency of its transmembrane transport into the cells by up to 15 times. A mutant form of Prdx6-S32A with an inactivated phospholipase center turned out to be unable to enter the cells in both the reduced and oxidized state of the peroxidase active center. Previously, we have shown that exogenous Prdx6 has a significant radioprotective action. However, the role of phospholipase activity in the radioprotective effects of Prdx6 remained unstudied. Trials with the mutant Prdx6-S32A form, with the use of a total irradiation model in mice, showed a nearly 50% reduction of the radioprotective effect upon aiPLA2 loss. Such a significant decrease in the radioprotective action may be due to the inability of Prdx6-S32A to penetrate animal cells, which prevents its reduction by the natural intracellular reducing agent glutathione S-transferase (πGST) and lowers the efficiency of elimination of peroxides formed from the effect of ionizing radiation. Thus, phospholipase activity may play an important role in the reduction of oxidized Prdx6 and manifestation of its antioxidant properties.

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Section: Discussionmentioning

confidence: 99%

The Role of Phospholipase Activity of Peroxiredoxin 6 in Its Transmembrane Transport and Protective Properties

Шарапов

Гончаров

Parfenyuk

et al. 2022

IJMS

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“…Experiments in animal models of diseases accompanied by development of oxidative stress showed that application of exogenous Prdx6 can correct the pathologic process course and facilitate faster recovery of damaged organs and tissues [103,106,107,108]. Particularly, exogenous Prdx6 application alleviates the severity of ionizing radiation damage, increasing the survival rate of animals exposed to sublethal and lethal doses [110,111].…”

Section: Discussionmentioning

confidence: 99%

“…Infusion of Prdx6 before the action of the aforementioned factors provides suppression of oxidative stress, thus preventing the damage of actively proliferating tissues. Prdx6 infusion after the damage, for example, after thermal burns of the upper respiratory tract, leads to a faster recovery of tissues, suppression of cell necrosis, and apoptosis [108,109]. Thus, the obtained experimental data has evidenced that exogenous Prdx6 possesses a high antioxidant potential and could be promising in the treatment of pathologies accompanied by the development of oxidative stress, particularly, those caused by ionizing radiation.…”

Section: Application Of Exogenous Prdx6 As a Radioprotectormentioning

confidence: 99%

Radioprotective Role of Peroxiredoxin 6

2019

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Peroxiredoxin 6 (Prdx6) is a member of an evolutionary ancient family of peroxidase enzymes with diverse functions in the cell. Prdx6 is an important enzymatic antioxidant. It reduces a wide range of peroxide substrates in the cell, thus playing a leading role in the maintenance of the redox homeostasis in mammalian cells. Beside peroxidase activity, Prdx6 has been shown to possess an activity of phospholipase A2, an enzyme playing an important role in membrane phospholipid metabolism. Moreover, Prdx6 takes part in intercellular and intracellular signal transduction due to its peroxidase and phospholipase activity, thus facilitating the initiation of regenerative processes in the cell, suppression of apoptosis, and activation of cell proliferation. Being an effective and important antioxidant enzyme, Prdx6 plays an essential role in neutralizing oxidative stress caused by various factors, including action of ionizing radiation. Endogenous Prdx6 has been shown to possess a significant radioprotective potential in cellular and animal models. Moreover, intravenous infusion of recombinant Prdx6 to animals before irradiation at lethal or sublethal doses has shown its high radioprotective effect. Exogenous Prdx6 effectively alleviates the severeness of radiation lesions, providing normalization of the functional state of radiosensitive organs and tissues, and leads to a significant elevation of the survival rate of animals. Prdx6 can be considered as a potent and promising radioprotective agent for reducing the pathological effect of ionizing radiation on mammalian organisms. The radioprotective properties and mechanisms of radioprotective action of Prdx6 are discussed in the current review.

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Protective role of exogenous recombinant peroxiredoxin 6 under ischemia-reperfusion injury of kidney

et al. 2019

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Effects of Different Antioxidant Enzymes on the Tracheal Epithelium Regeneration After Chemical Burn

Cited by 8 publications

References 8 publications

The Role of Phospholipase Activity of Peroxiredoxin 6 in Its Transmembrane Transport and Protective Properties

The Role of Phospholipase Activity of Peroxiredoxin 6 in Its Transmembrane Transport and Protective Properties

Radioprotective Role of Peroxiredoxin 6

Protective role of exogenous recombinant peroxiredoxin 6 under ischemia-reperfusion injury of kidney

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