Effects of different calcium sources on the mineralization and sand curing of CaCO<sub>3</sub>by carbonic anhydrase-producing bacteria

Pan, Ling; Li, Qiongfang; Zhou, Yi; Song, Na; Yu, Lujia; Wang, Xuhui; Xiong, Ke; Yap, LikSen; Huo, Jianlin

doi:10.1039/c9ra09025h

Cited by 27 publications

(11 citation statements)

References 44 publications

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“…Because cementitious system's internal pH is higher, pH drops to 7 at the outer face where surface cracks occur (Lors et al, 2017). As calcite deposition by active pathway increased the system pH (Pan et al, 2019). So, change in pH at the end of incubation period was also monitored.…”

Section: Effect Of Varying Ph On Calcite Precipitationmentioning

confidence: 99%

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO₃ precipitation in bio‐based cementitious composites

Shaheen

Jalil

Adnan

et al. 2021

Microbial Biotechnology

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Microbially induced calcite precipitation (MICP), secreted through biological metabolic activity, secured an imperative position in remedial measures within the construction industry subsequent to ecological, environmental and economical returns. However, this contemporary recurrent healing system is susceptible to microbial depletion in the highly alkaline cementitious environment. Therefore, researchers are probing for alkali resistant calcifying microbes. In the present study, alkaliphilic microbes were isolated from different soil sources and screened for probable CaCO 3 precipitation. Non-ureolytic pathway (oxidation of organic carbon) was adopted for calcite precipitation to eliminate the production of toxic ammonia. For this purpose, calcium lactate Ca(C 3 H 5 O 3 ) 2 and calcium acetate Ca (CH 3 COO) 2 were used as CaCO 3 precipitation precursors. The quantification protocol for precipitated CaCO 3 was established to select potent microbial species for implementation in the alkaline cementitious systems as more than 50% of isolates were able to precipitate CaCO 3 . Results suggested 80% of potent calcifying strains isolated in this study, portrayed higher calcite precipitation at pH 10 when compared to pH 7. Ten superlative morphologically distinct isolates capable of CaCO 3 production were identified by 16SrRNA sequencing. Sequenced microbes were identified as species of Bacillus, Arthrobacter, Planococcus, Chryseomicrobium and Corynebacterium. Further, microstructure of precipitated CaCO 3 was inspected through scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric (TG) analysis. Then, the selected microbes were investigated in the cementitious mortar to rule out any detrimental effects on mechanical properties. These strains showed maximum of 36% increase in compressive strength and 96% increase in flexural strength.Bacillus, Arthrobacter, Corynebacterium and Planococcus genera have been reported as CaCO 3 producers but isolated strains have not yet been investigated in conjunction with cementitious mortar. Moreover, species of Chryseomicrobium and Glutamicibacter were reported first time as calcifying strains.

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Section: Effect Of Varying Ph On Calcite Precipitationmentioning

confidence: 99%

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO₃ precipitation in bio‐based cementitious composites

Shaheen

Jalil

Adnan

et al. 2021

Microbial Biotechnology

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“…The potent bacterium's in-vitro calcium carbonates formation competence was examined by culturing bacteria on nutrient agar plates supplemented with 25…”

Section: Preparation Of Deposition Systemmentioning

confidence: 99%

Effect of Different Calcium Salts on Calcium Carbonates Formation Induced by Halophilic Bacillus oceanisediminis CB1

et al. 2022

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Biomineralization through the biomimetic CO2 sequestration process has been gaining attraction in recent years due to the formation of carbonates widely used as raw material in various industrial processes. The deposition and dissolution of calcium carbonate can be affected by physiochemical factors, such as the type of calcium salt. However, most studies have focused on calcium chloride (CaCl2). In the present study, A potent bacterial carbonic anhydrase (CA) producer, Bacillus oceanisediminis CB1, was screened on CA activity from mangrove plant Avicennia marina, collected from Ghogha, Bhavnagar, India (21.68°N 72.28°E). We premeditated deposition experiments to determine the effects of different calcium salts on calcium carbonate deposition in Bacillus oceanisediminis CB1 colonies. The results demonstrated the calcite formation observed in calcium salt-supplemented nutrient agar, calcium chloride, and calcium acetate. Merely uniform distribution and peripheral distribution of calcite particles found in calcium acetate and calcium chloride supplemented into nutrient agar, respectively. Calcite formation was confirmed by staining with Alizarin Red S dye followed by SEM-EDX. This study will provide a vital reference for designing and applying microbial-induced carbonate precipitation using different calcium salts.

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“…Different researchers have studied the influence of calcium salts on MICP technology and obtained different research results. Pan et al [156] compared the effects of calcium salts CaCl 2 , Ca(NO 3 ) 2, and Ca(CH 3 COO) 2 on Bacillus cereus solidified sand. The results showed that the CaCO 3 content precipitated from CaCl 2 and Ca(CH 3 COO) 2 as calcium sources were relatively high, while Ca(NO 3 ) 2 as a calcium source produced more dense and uniform precipitation and greater compressive strength.…”

Section: Sources Of Calciummentioning

confidence: 99%

Critical Review of Solidification of Sandy Soil by Microbially Induced Carbonate Precipitation (MICP)

et al. 2021

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Microbially induced carbonate precipitation (MICP) is a promising technology for solidifying sandy soil, ground improvement, repairing concrete cracks, and remediation of polluted land. By solidifying sand into soil capable of growing shrubs, MICP can facilitate peak and neutralization of CO2 emissions because each square meter of shrub can absorb 253.1 grams of CO2 per year. In this paper, based on the critical review of the microbial sources of solidified sandy soil, models used to predict the process of sand solidification and factors controlling the MICP process, current problems in microbial sand solidification are analyzed and future research directions, ideas and suggestions for the further study and application of MICP are provided. The following topics are considered worthy of study: (1) MICP methods for evenly distributing CaCO3 deposit; (2) minimizing NH4+ production during MICP; (3) mixed fermentation and interaction of internal and exogenous urea-producing bacteria; (4) MICP technology for field application under harsh conditions; (5) a hybrid solidification method by combining MICP with traditional sand barrier and chemical sand consolidation; and (6) numerical model to simulate the erosion resistance of sand treated by MICP.

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Effects of different calcium sources on the mineralization and sand curing of CaCO₃by carbonic anhydrase-producing bacteria

Abstract: The deposition and dissolution of calcium carbonate can be affected by the action of biological factors, such as microbial-induced carbonate precipitation (MICP).

Cited by 27 publications

References 44 publications

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO₃ precipitation in bio‐based cementitious composites

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO₃ precipitation in bio‐based cementitious composites

Effect of Different Calcium Salts on Calcium Carbonates Formation Induced by Halophilic Bacillus oceanisediminis CB1

Critical Review of Solidification of Sandy Soil by Microbially Induced Carbonate Precipitation (MICP)

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Effects of different calcium sources on the mineralization and sand curing of CaCO3by carbonic anhydrase-producing bacteria

Abstract: The deposition and dissolution of calcium carbonate can be affected by the action of biological factors, such as microbial-induced carbonate precipitation (MICP).

Cited by 27 publications

References 44 publications

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO3 precipitation in bio‐based cementitious composites

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO3 precipitation in bio‐based cementitious composites

Effect of Different Calcium Salts on Calcium Carbonates Formation Induced by Halophilic Bacillus oceanisediminis CB1

Critical Review of Solidification of Sandy Soil by Microbially Induced Carbonate Precipitation (MICP)

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Effects of different calcium sources on the mineralization and sand curing of CaCO₃by carbonic anhydrase-producing bacteria

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO₃ precipitation in bio‐based cementitious composites

Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO₃ precipitation in bio‐based cementitious composites