2019
DOI: 10.1016/j.cryobiol.2018.12.005
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Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds

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Cited by 25 publications
(16 citation statements)
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“…In this study, no superiority was observed between the use of GL (Soni et al, 2019). Yang et al (2016) evaluated the effect of GL with dimethylacetamide and detected that the combined use of them did not enhance the cryosurvival of boar spermatozoa.…”
Section: (%) Lr (%) DI (%) Dr (%)mentioning
confidence: 66%
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“…In this study, no superiority was observed between the use of GL (Soni et al, 2019). Yang et al (2016) evaluated the effect of GL with dimethylacetamide and detected that the combined use of them did not enhance the cryosurvival of boar spermatozoa.…”
Section: (%) Lr (%) DI (%) Dr (%)mentioning
confidence: 66%
“…To the authors' knowledge, there is no report on the use of combination of CPs (DMF and GL) on cryopreserved camelid semen. In other species, such as stallions, same results were found on frozen semen, with no superiority when GL and DMF were tested combined compared to the single use of them (GL 2.5% plus DMF 2.5%; GL 1% plus DMF 1% versus GL 5%; DMF 5%) on sperm viability, acrosome status or DNA quality (Soni et al., 2019). Yang et al.…”
Section: Discussionmentioning
confidence: 80%
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“…Although penetrating cryoprotectants can increase cell membrane fluidity through protein and lipid reorganization [5], they can also hamper intracellular ice crystal formation by decreasing the freezing point temperature, which is known to be an undesirable mechanism of sperm death [6,7]. The sperm cryopreservation process involves changes of extreme temperature that can lead to cryoinjuries (decreases of plasma membrane integrity, acrosomal integrity, mitochondrial membrane potential, motility, and viability) of morphological structures and physiology [8][9][10]. These cryoinjuries can alter the functional status of sperm proteins in the plasma membrane, midpiece, nucleus, and flagella, thus affecting the motility and fertilization capacity of sperm [11][12][13][14][15].…”
Section: Introductionmentioning
confidence: 99%
“…Besides the need for cryoprotectants, there is also a need to add cryo‐supplement. These cryo‐supplements have antioxidant effects and are used to improve sperm parameters 7,22 . Trehalose is a disaccharide as an energy source that consists of two D‐glucose molecules.…”
Section: Introductionmentioning
confidence: 99%