Effects of different dietary vegetable oils on growth and intestinal performance, lipid metabolism and flesh quality in gilthead sea bream

Sánchez-Moya, Albert; García-Meilán, Irene; Riera‐Heredia, Natàlia; Vélez, Emilio J.; Fontanillas, Ramón; Gutiérrez, Joaquím; Capilla, Encarnación; Navarro, Isabel

doi:10.1016/j.aquaculture.2019.734881

Cited by 31 publications

(35 citation statements)

References 78 publications

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“…In a lean species such as European sea bass, the use of a high EPA+DHA genetically modified- Camelina sativa oil has instead been shown to promote adequate growth even at moderate dietary inclusion of FM (i.e., 25%) ( Betancor et al, 2021 ). In the present study, FM was included at 19.4%, with no differences observed in growth with a 40% substitution of FO by CO. Other studies in the same species, did not find an effect on growth or feed utilization when FO was replaced up to 75% with VO at FM levels between 15–20% ( Sánchez-Moya et al, 2020 ). The poorest growth in fish fed with 60% CO could be due to the presence of glucosinolates, which are responsible for the bitter or sharp taste of many cruciferous vegetables ( Clarke, 2010 ) and are known to be prevalent in CO ( Berhow et al, 2013 ).…”

Section: Discussionmentioning

confidence: 88%

Effect of dietary oil fromCamelina sativaon the growth performance, fillet fatty acid profile and gut microbiome of gilthead Sea bream (Sparus aurata)

Huyben

Rimoldi

Ceccotti

et al. 2020

PeerJ

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Background In the last two decades, research has focused on testing cheaper and sustainable alternatives to fish oil (FO), such as vegetable oils (VO), in aquafeeds. However, FO cannot be entirely replaced by VOs due to their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA), particularly eicosapentaenoic (EPA; 20:5n-3) and docosahexaenoic (DHA; 22:6n-3) acids. The oilseed plant, Camelina sativa, may have a higher potential to replace FO since it can contains up to 40% of the omega-3 precursors α-linolenic acid (ALA; 18:3n-3) and linoleic acid (LA; 18:2n-6). Methods A 90-day feeding trial was conducted with 600 gilthead sea bream (Sparus aurata) of 32.92 ± 0.31 g mean initial weight fed three diets that replaced 20%, 40% and 60% of FO with CO and a control diet of FO. Fish were distributed into triplicate tanks per diet and with 50 fish each in a flow-through open marine system. Growth performance and fatty acid profiles of the fillet were analysed. The Illumina MiSeq platform for sequencing of 16S rRNA gene and Mothur pipeline were used to identify bacteria in the faeces, gut mucosa and diets in addition to metagenomic analysis by PICRUSt. Results and Conclusions The feed conversion rate and specific growth rate were not affected by diet, although final weight was significantly lower for fish fed the 60% CO diet. Reduced final weight was attributed to lower levels of EPA and DHA in the CO ingredient. The lipid profile of fillets were similar between the dietary groups in regards to total saturated, monounsaturated, PUFA (n-3 and n-6), and the ratio of n-3/n-6. Levels of EPA and DHA in the fillet reflected the progressive replacement of FO by CO in the diet and the EPA was significantly lower in fish fed the 60% CO diet, while ALA was increased. Alpha and beta-diversities of gut bacteria in both the faeces and mucosa were not affected by any dietary treatment, although a few indicator bacteria, such as Corynebacterium and Rhodospirillales, were associated with the 60% CO diet. However, lower abundance of lactic acid bacteria, specifically Lactobacillus, in the gut of fish fed the 60% CO diet may indicate a potential negative effect on gut microbiota. PICRUSt analysis revealed similar predictive functions of bacteria in the faeces and mucosa, although a higher abundance of Corynebacterium in the mucosa of fish fed 60% CO diet increased the KEGG pathway of fatty acid synthesis and may act to compensate for the lack of fatty acids in the diet. In summary, this study demonstrated that up to 40% of FO can be replaced with CO without negative effects on growth performance, fillet composition and gut microbiota of gilthead sea bream.

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Section: Discussionmentioning

confidence: 88%

Effect of dietary oil fromCamelina sativaon the growth performance, fillet fatty acid profile and gut microbiome of gilthead Sea bream (Sparus aurata)

Huyben

Rimoldi

Ceccotti

et al. 2020

PeerJ

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“…Research in aquaculture has been investigating new sustainable strategies to face the lowered pelagic stocks to produce fish feeds, using VO to substitute FO. The different dietary FA profiles in these oils have shown to differentially affect fish lipid metabolism and adiposity [17,25,28]. In this context, the relevance of testing the direct effects of specific FA, in vitro as well as in vivo, must be taken into account to obtain a general view of the importance of FO substitution in fish lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…In adipocyte cultures (n = 6) the cells were lysed with a cell scraper and TRI Reagent (Applied Biosystems, Alcobendas, Spain) in a total volume of 1 mL per each two wells [31]. For tissue samples, 100 mg of adipose tissue (n = 9) and 50 mg of liver (n = 8) were homogenated in 1 mL of TRI Reagent, using Precellys Evolution (Bertin Instruments, Montigny-le-Bretonneux, France) [28]. Total RNA was extracted according to the manufacturer's recommendations, dissolved in DEPC-treated water (RNase-free), quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Alcobendas, Spain), and stored at −80 • C. To eliminate any residual genomic DNA, total RNA (1 µg) was treated with DNase I (Invitrogen, Alcobendas, Spain) and converted into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Sant Cugat del Valles, Spain), following the manufacturer's instructions.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout (Oncorhynchus mykiss)

Riera‐Heredia

Lutfi

Sánchez-Moya

et al. 2020

IJMS

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Fish are rich in n-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Due to the increasing use of vegetable oils (VO), their proportion in diets has lowered, affecting lipid metabolism and fillet composition. Rainbow trout cultured preadipocytes were treated with representative FA found in fish oils (EPA and DHA) or VO (linoleic, LA and alpha-linolenic, ALA acids), while EPA and LA were also orally administered, to evaluate their effects on adipogenesis and lipid metabolism. In vitro, all FA increased lipid internalization, with ALA producing the highest effect, together with upregulating the FA transporter fatp1. In vivo, EPA or LA increased peroxisome proliferator-activated receptors ppara and pparb transcripts abundance in adipose tissue, suggesting elevated β-oxidation, contrary to the results obtained in liver. Furthermore, the increased expression of FA synthase (fas) and the FA translocase/cluster of differentiation (cd36) in adipose tissue indicated an enhanced uptake of lipids and lipogenesis de novo, whereas stable or low hepatic expression of genes involved in lipid transport and turnover was found. Thus, fish showed a similar tissue metabolic response to the short-term availability of EPA or LA in vivo, while in vitro VO-derived FA demonstrated greater potential inducing fat accumulation.

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“…Thus, reducing their use and developing new fish diets with alternative nutrient sources are nowadays recognized as a priority for the future growth and sustainability of the industry [ 1 ]. In this regard, although vegetable oils are relatively poor sources of n-3 fatty acids in comparison to FO [ 2 ], they have been incorporated as alternative ingredients in fish diets with successful results [ 3 , 4 , 5 , 6 ]. Among them, soybean oil has been one of the most used as a non-fish source of n-6 and n-3 polyunsaturated fatty acids [ 2 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

Balbuena-Pecino

Lutfi

Riera‐Heredia

et al. 2020

IJMS

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Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout.

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Effects of different dietary vegetable oils on growth and intestinal performance, lipid metabolism and flesh quality in gilthead sea bream

Cited by 31 publications

References 78 publications

Effect of dietary oil fromCamelina sativaon the growth performance, fillet fatty acid profile and gut microbiome of gilthead Sea bream (Sparus aurata)

Effect of dietary oil fromCamelina sativaon the growth performance, fillet fatty acid profile and gut microbiome of gilthead Sea bream (Sparus aurata)

Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout (Oncorhynchus mykiss)

Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

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