Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes

Dias, JH; Gonçalves, JD; Arrais, AM; Souza-Fabjan, J. M. G.; Bastos, Rosemary; Batista, RITP; Siqueira, LGB; Oliveira, MEF; Fonseca, JF

doi:10.1016/j.domaniend.2022.106751

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…The number of CL ranged from 5 to 13 units, the average embryos for donors (days 2 and 6) was 7.53, and transferable embryos-5.84. These results were similar to the results of other researchers [24] and even slightly exceeded them [25]. Dias et al used superovulation protocol that included an intravaginal device (0.36 g of progesterone) for nine days plus six decreasing doses of pFSH (333 IU, from 60 h before intravaginal device removal) and two doses of 37.5µg d-cloprostenol at the time of last two pFSH doses.…”

Section: Discussionsupporting

confidence: 83%

“…Dias et al used superovulation protocol that included an intravaginal device (0.36 g of progesterone) for nine days plus six decreasing doses of pFSH (333 IU, from 60 h before intravaginal device removal) and two doses of 37.5µg d-cloprostenol at the time of last two pFSH doses. The effectiveness of the protocol was in the range of 7.0-8.6 in the number of CL, and the collected embryos-3.7-6.2 per donor [24]. Figueira et al to induce superovulation in donors used acetate medroxyprogesterone intravaginal sponges for six or nine days plus d-cloprostenol and eCG 24 h before sponge removal and conclude that the nine-day protocol promoted higher ovulation rate and embryo yield [25].…”

Section: Discussionmentioning

confidence: 94%

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Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques

Selionova

Aibazov

Zharkova

2023

Animals

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This article presents data from experiments to determine the cryoresistance of Charollais sheep embryos, depending on the stage of embryo development and the method of freezing, as well as the results of embryo transfer. The study design consisted of a study on the cryopreservation of ewe embryos at different developmental stages (early, 2–8 blastomeric and late, at the morula/blastocyst stage), two cryopreservation protocols (slow freezing and ultra-fast vitrification), and embryo transfer of cryo- and fresh embryos. Embryos from Charollais sheep donors (n = 12) were recovered after induction of superovulation. The embryos were recovered surgically (laparotomy) on days 2 and 6 after insemination. Before there was transfer to recipients, part of embryos was cryopreserved using standard slow freezing and ultra-fast vitrification methods. The average ovarian response was 7.54 ovulations per donor, and 5.83 embryos per donor were collected. No effect of the cryopreservation method and embryo development stage on the preservation of the morphological structure of embryos was found. There were no significant differences in the survival rate of cryoembryos at different development stages, frozen using different techniques, and after transfer to recipients. Differences in cryoresistance between embryonic developmental stages in favor of the morula/blastocyst stage were found (survival after thawing 86.4% vs. 75.0% in early embryos). At different stages of development, the survival rate of fresh embryos (45.8%) compared to cryopreserved ones (30.2%) was significantly higher (p < 0.05), while among fresh ones, the best survival rate (50.0%) was observed after the transfer of morules and blastocysts.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 94%

Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques

Selionova

Aibazov

Zharkova

2023

Animals

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“…Despite the good outcomes and still being the most commercially used method for sheep, surgical procedures for embryo collection involve several animal welfare issues due to the anaesthetic and surgical risks and a limit of successive donor uses as a consequence of post‐surgical sequelae in reproductive organs (Fonseca et al, 2016; Gomes et al, 2014; Pinto et al, 2020). However, although there are initial studies on pharmacological protocols for cervical dilation (Dias et al, 2023, 2020; Fonseca, Zambrini, et al, 2019; Leite et al, 2018; Prellwitz et al, 2019), the complex sheep's cervical morphology makes transcervical uterine access difficult (Fonseca et al, 2019a; Kershaw et al, 2005). Although non‐surgical embryo recovery (NSER) is less invasive and has good recovery rates of structures, under some drugs preparations/combination and anaesthetic procedures used, it also induces an elevation of inflammatory markers (Oliveira et al, 2018), heart rate, cortisol values and glycaemia (Santos et al, 2020) indicative of stress, and probably pain and discomfort generated by cervical manipulation.…”

Section: Introductionmentioning

confidence: 99%

Use of meloxicam with or without dipyrone in non‐surgical embryo recovery in hair sheep: Effects on animal welfare

Ribeiro

Taira

Santos

et al. 2023

Reprod Domestic Animals

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The aim of this study was to determine the effectiveness of meloxicam with or without dipyrone on the welfare of ewes subjected to non‐surgical embryo recovery (NSER). Two studies were carried out using 51 multiparous Santa Inês ewes. All animals received a standard oestrous synchronization treatment and a superovulatory protocol. In Study 1, 12 ewes received meloxicam (GM) before cervical transposition (1 mg kg−1, i.v.), repeated 24 h after (1 mg kg−1, i.m.), while the other 10 received a saline solution, remaining as a control group (GC1). In Study 2, ewes were allocated into a group of 15 ewes treated as GM of Study 1 associated with dipyrone (GMD; 50 mg kg−1, i.m.) before cervical transposition, 12 h, and 24 h after, or a control group (GC2) of 14 ewes treated with saline solution. In both studies, heart and respiratory rates (RR), cortisol, glucose, total proteins, albumin and globulins blood concentration were recorded before sedation (BS), after sedation (AS), after cervical transposition, immediately after collection (IAC), and 0.5, 1.5, 3, 6, 12, 24 and 48 h after embryo collection (hAC). In Study 1, RR tended to be greater in GC1 (p = .08), serum total proteins and globulins values were lower and serum albumin values were greater in this group than GM (p = .003, p < .0001, and p < .0001, respectively). In Study 2, treatment of GMD tended to reduce the glycaemia at AS (p = .052) and reduced it at 3hAC (p < .0001), and 6hAC (p = .03). It also tended to reduce cortisol concentrations (p = .10). The other variables varied with NSER without interaction with the experimental treatments. In conclusion, in this study condition, NSER in sheep induced transient changes indicative of stress and possibly pain, therefore, affecting animal welfare. The administration of meloxicam was ineffective to reduce those responses, and the association of dipyrone had only slight effects without modifying the main welfare indicative responses in ewes subjected to NSER.

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Review: Non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants

Souza-Fabjan

Oliveira

Guimarães

et al. 2023

animal

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Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes

Cited by 5 publications

References 30 publications

Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques

Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques

Use of meloxicam with or without dipyrone in non‐surgical embryo recovery in hair sheep: Effects on animal welfare

Review: Non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants

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