Effects of Different Elicitors on Micropropagation, Biomass and Secondary Metabolite Production of Stevia rebaudiana Bertoni—A Review

Miladinova-Georgieva, Kamelia; Geneva, Maria; Stancheva, Ira; Petrova, Maria; Sichanova, Mariana; Kirova, Elisaveta

doi:10.3390/plants12010153

Cited by 21 publications

(18 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method can be used to produce economically viable SMs on a large spectrum level. Elicitors act as a signaling molecule and these signals identified by the plant cellular membrane-bound receptors and activation of the signal transduction pathways and change the expression of regulatory players and resulting in enhanced synthesis and accumulation of phytocompounds (Zhai et al 2017 ; Miladinova-Georgieva et al 2023 ). Employing in vitro cell culture as a model system, it may also be used to characterize and investigate the impact of various elicitors on plants.…”

Section: Elicitation Is a Key Tool To Ameliorate The Active Metabolitesmentioning

confidence: 99%

Methyl jasmonate and salicylic acid as powerful elicitors for enhancing the production of secondary metabolites in medicinal plants: an updated review

Jeyasri

Muthuramalingam

Karthick

et al. 2023

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Plant secondary metabolites are bioactive scaffolds that are crucial for plant survival in the environment and to maintain a defense mechanism from predators. These compounds are generally present in plants at a minimal level and interestingly, they are found to have a wide variety of therapeutic values for humans. Several medicinal plants are used for pharmaceutical purposes due to their affordability, fewer adverse effects, and vital role in traditional remedies. Owing to this reason, these plants are exploited at a high range worldwide and therefore many medicinal plants are on the threatened list. There is a need of the hour to tackle this major problem, one effective approach called elicitation can be used to enhance the level of existing and novel plant bioactive compounds using different types of elicitors namely biotic and abiotic. This process can be generally achieved by in vitro and in vivo experiments. The current comprehensive review provides an overview of biotic and abiotic elicitation strategies used in medicinal plants, as well as their effects on secondary metabolites enhancement. Further, this review mainly deals with the enhancement of biomass and biosynthesis of different bioactive compounds by methyl jasmonate (MeJA) and salicylic acid (SA) as elicitors of wide medicinal plants in in vitro by using different cultures. The present review was suggested as a significant groundwork for peers working with medicinal plants by applying elicitation strategies along with advanced biotechnological approaches.

show abstract

Section: Elicitation Is a Key Tool To Ameliorate The Active Metabolitesmentioning

confidence: 99%

Methyl jasmonate and salicylic acid as powerful elicitors for enhancing the production of secondary metabolites in medicinal plants: an updated review

Jeyasri

Muthuramalingam

Karthick

et al. 2023

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…are utilized in various plants. Despite the proven effectiveness of elicitation techniques [ 104 , 105 , 106 , 107 , 108 , 109 , 110 ], research in this area on E. longifolia is insufficient.…”

Section: Future Perspectivesmentioning

confidence: 99%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

View full text Add to dashboard Cite

Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, various techniques have been used to mass propagate and improve the yield of these bioactive compounds in tissue cultures. These techniques include the optimization of media conditions and application of various types and combinations of plant growth regulators (PGRs). In addition, some elicitation techniques have been used to improve the synthesis of these bioactive compounds. However, in comparison with other herbal species with similar economic importance, many techniques have not been applied to E. longifolia. Adopting the most recent methodologies would ensure efficiency and sustainability in the in vitro production of bioactive compounds in E. longifolia. Therefore, in this review, we present an up-to-date record on the success stories in the tissue culture techniques and synthesis of bioactive compounds. In addition, we attempted to identify some of the missing links on the road to the effective and sustainable biotechnological utilization of this super important biological resource.

show abstract

“…The employment of biotic or abiotic elicitors has emerged as a vital biotechnological approach to drive mass production and obtain the desired metabolite concentrations. Elicitors are a group of compounds that trigger defense mechanisms in plants and result in the formation of secondary metabolites [ 9 , 10 ]. These compounds also act as antioxidants and scavenge free radicals and reactive oxygen species [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

Kanthaliya

Joshi

Arora

et al. 2023

Plants

View full text Add to dashboard Cite

Pueraria tuberosa contains a wide range of bioactive compounds, including polyphenols, alkaloids, and phytosterols, which make it valuable to the pharmaceutical and food industries. Elicitor compounds trigger the defense mechanisms in plants and are widely used to increase the yield of bioactive molecules in in vitro cultures. The present study was conducted to evaluate the effects of different concentrations of biotic elicitors such as yeast extract (YE), pectin (PEC), and alginate (ALG) on growth, antioxidant activity, and metabolite accumulation in in vitro propagated shoots of P. tuberosa. The elicitors applied to shoot cultures of P. tuberosa significantly increased biomass (shoot number, fresh weight, and dry weight), and metabolites such as protein, carbohydrates, chlorophyll, total phenol (TP), and total flavonoid (TF) contents, as well as antioxidant activity compared to untreated control. Biomass, TP, and TF contents, as well as antioxidant activity, were most significant in cultures treated with 100 mg/L PEC. In contrast, chlorophyll, protein, and carbohydrate increased most in cultures treated with 200 mg/L ALG. Application of 100 mg/L of PEC led to the accumulation of high amounts of isoflavonoids including puerarin (220.69 μg/g), daidzin (2935.55 μg/g), genistin (5612 μg/g), daidzein (479.81 μg/g), and biochanin-A (111.511 μg/g) as analyzed by high-performance liquid chromatography (HPLC). Total isoflavonoids content of 100 mg/L PEC treated shoots was obtained as 9359.56 μg/g, 1.68-fold higher than in vitro propagated shoots without elicitors (5573.13 μg/g) and 2.77-fold higher than shoots of the mother plant (3380.17 μg/g). The elicitor concentrations were optimized as 200 mg/L YE, 100 mg/L PEC, and 200 mg/L ALG. Overall, this study showed that the application of different biotic elicitors resulted in better growth, antioxidant activity, and accumulation of metabolites in P. tuberosa, which could lead to obtaining phytopharmaceutical advantages in the future.

show abstract

Effects of Different Elicitors on Micropropagation, Biomass and Secondary Metabolite Production of Stevia rebaudiana Bertoni—A Review

Cited by 21 publications

References 106 publications

Methyl jasmonate and salicylic acid as powerful elicitors for enhancing the production of secondary metabolites in medicinal plants: an updated review

Methyl jasmonate and salicylic acid as powerful elicitors for enhancing the production of secondary metabolites in medicinal plants: an updated review

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

Contact Info

Product

Resources

About