Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyl transferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells

Abstract

The effects of four elicitors, including 100 mmol/l MeJA (methyl jasmonate), 40 ml/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content

“…The significant increase in the levels of acetylated diterpene and sterol viz ., sarcophytonolide and cholesteryl acetate, suggest an activation of specific acetyl transferases as part of the defence response to ZnCl 2 and SA. This hypothesis is supported by previous findings in which elicitation of Taxus cuspidata with SA was found to increase the content of 10-deacetylbaccatin III-10- O -acetyltransferase (10-DBAT); an enzyme that catalyses the formation of baccatin II, and ultimately promoting the production of taxol 57 . The relative change in sarcophytonolide I and cholesteryl acetate levels, presumably on the expense of other terpenoids, negates a de novo biosynthetic activation in response to elicitation but is likely to involve downstream acetylation reaction activation.…”

Enhancement of Taxol production by applying amino acid complex along with chitosan in suspension culture of Taxus baccata L.

“…different elicitors have been applied in considerable research efforts. For example, boosting paclitaxel production by adding SA to the cell culture medium of T. chinensis [27]; improving yields of this taxane in a two-stage cell suspension culture of T. baccata using two different concentrations of SA [21]; enhancing yields of paclitaxel and other taxane with various fungal extracts, alone or in combination with other elicitors [28][29]; increasing Taxol biosynthesis and excretion to the medium 55 times higher than non-elicited cultures of T. media [30]; increasing in Taxol (5.1-fold) and total taxanes (3.5-fold) in the SApretreated calli of T. baccata cultured on the medium containing 2 % glucose, compared to the control [18] and improving Taxol production (2.5 times more than the control) by applying polyethylene glycol (3 %) in a T. baccata L. callus cultures [31]. Chitosan is another elicitor which has been used to improve paclitaxel production in cell suspension cultures of T. chinensis [32][33].…”