Effects of different excitation and detection spectral regions on room temperature chlorophyll a fluorescence parameters

Štroch, Michal; Podolinská, Jana; Navrátil, Martin; Špunda, Vladimı́r

doi:10.1007/s11099-005-0065-3

Cited by 7 publications

(1 citation statement)

References 20 publications

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“…Possible interpretations of the above results can be found by considering the now accepted evidence that the ChlF emission spectrum receives significant contributions from both PSII and PSI photosystems (PSs), even at physiological temperatures (Genty et al 1990;Pfündel 1998;Agati et al 2000;Peterson et al 2001;Š troch et al 2005). Nevertheless, the contribution of PSI is mainly at the longer wavelength emission band, at about 735 nm, while PSII contributes to both the ChlF emission bands.…”

Section: Introductionmentioning

confidence: 75%

A retrieval algorithm to evaluate the Photosystem I and Photosystem II spectral contributions to leaf chlorophyll fluorescence at physiological temperatures

et al. 2011

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A new computational procedure to resolve the contribution of Photosystem I (PSI) and Photosystem II (PSII) to the leaf chlorophyll fluorescence emission spectra at room temperature has been developed. It is based on the Principal Component Analysis (PCA) of the leaf fluorescence emission spectra measured during the OI photochemical phase of fluorescence induction kinetics. During this phase, we can assume that only two spectral components are present, one of which is constant (PSI) and the other variable in intensity (PSII). Application of the PCA method to the measured fluorescence emission spectra of Ficus benjamina L. evidences that the temporal variation in the spectra can be ascribed to a single spectral component (the first principal component extracted by PCA), which can be considered to be a good approximation of the PSII fluorescence emission spectrum. The PSI fluorescence emission spectrum was deduced by difference between measured spectra and the first principal component. A single-band spectrum for the PSI fluorescence emission, peaked at about 735 nm, and a 2-band spectrum with maxima at 685 and 740 nm for the PSII were obtained. A linear combination of only these two spectral shapes produced a good fit for any measured emission spectrum of the leaf under investigation and can be used to obtain the fluorescence emission contributions of photosystems under different conditions. With the use of our approach, the dynamics of energy distribution between the two photosystems, such as state transition, can be monitored in vivo, directly at physiological temperatures. Separation of the PSI and PSII emission components can improve the understanding of the fluorescence signal changes induced by environmental factors or stress conditions on plants.

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Section: Introductionmentioning

confidence: 75%

A retrieval algorithm to evaluate the Photosystem I and Photosystem II spectral contributions to leaf chlorophyll fluorescence at physiological temperatures

et al. 2011

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Pigment composition and functional state of the thylakoid membranes during preparation of samples for pigment-protein complexes separation by nondenaturing gel electrophoresis

Karlický¹,

Podolinská²,

Nadkanská³

et al. 2010

Photosynt.

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The present study was conducted to examine changes in photosynthetic pigment composition and functional state of the thylakoid membranes during the individual steps of preparation of samples that are intended for a separation of pigmentprotein complexes by nondenaturing polyacrylamide gel electrophoresis. The thylakoid membranes were isolated from barley leaves (Hordeum vulgare L.) grown under low irradiance (50 µmol m -2 s -1 ). Functional state of the thylakoid membrane preparations was evaluated by determination of the maximal photochemical efficiency of photosystem (PS) II (F V /F M ) and by analysis of excitation and emission spectra of chlorophyll a (Chl a) fluorescence at 77 K. All measurements were done at three phases of preparation of the samples: (1) in the suspensions of osmotically-shocked broken chloroplasts, (2) thylakoid membranes in extraction buffer containing Tris, glycine, and glycerol and (3) thylakoid membranes solubilized with a detergent decyl-β-D-maltosid. F V /F M was reduced from 0.815 in the first step to 0.723 in the second step and to values close to zero in solubilized membranes. Pigment composition was not pronouncedly changed during preparation of the thylakoid membrane samples. Isolation of thylakoid membranes affected the efficiency of excitation energy transfer within PSII complexes only slightly. Emission and excitation fluorescence spectra of the solubilized membranes resemble spectra of trimers of PSII light-harvesting complexes (LHCII). Despite a disrupted excitation energy transfer from LHCII to PSII antenna core in solubilized membranes, energy transfer from Chl b and carotenoids to emission forms of Chl a within LHCII trimers remained effective.Additional key words: 77-K chlorophyll fluorescence spectra; Hordeum vulgare; photochemical efficiency; polyacrylamide gel electrophoresis; thylakoid membranes isolation.

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Deriving fluorometer-specific values of relative PSI fluorescence intensity from quenching of F 0 fluorescence in leaves of Arabidopsis thaliana and Zea mays

Pfündel

Klughammer²,

Meister

et al. 2012

Photosynth Res

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The effect of stepwise increments of red light intensities on pulse-amplitude modulated (PAM) chlorophyll (Chl) fluorescence from leaves of A. thaliana and Z. mays was investigated. Minimum and maximum fluorescence were measured before illumination (F(0) and F(M), respectively) and at the end of each light step (F'(0) and F'(M), respectively). Calculated F'(0) values derived from F(0), F(M) and F'(M) fluorescence according to Oxborough and Baker (1997) were lower than the corresponding measured F'(0) values. Based on the concept that calculated F'(0) values are under-estimated because the underlying theory ignores PSI fluorescence, a method was devised to gain relative PSI fluorescence intensities from differences between calculated and measured F'(0). This method yields fluorometer-specific PSI data as its input data (F(0), F(M), F'(0) and F'(M)) depend solely on the spectral properties of the fluorometer used. Under the present conditions, the PSI contribution to F (0) fluorescence was 0.24 in A. thaliana and it was independent on the light acclimation status; the corresponding value was 0.50 in Z. mays. Correction for PSI fluorescence affected Z. mays most: the linear relationship between PSI and PSII photochemical yields was clearly shifted toward the one-to-one proportionality line and maximum electron transport was increased by 50 %. Further, correction for PSI fluorescence increased the PSII reaction center-specific parameter, 1/F(0) - 1/F(M), up to 50 % in A. thaliana and up to 400 % in Z. mays.

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Effects of different excitation and detection spectral regions on room temperature chlorophyll a fluorescence parameters

Cited by 7 publications

References 20 publications

A retrieval algorithm to evaluate the Photosystem I and Photosystem II spectral contributions to leaf chlorophyll fluorescence at physiological temperatures

A retrieval algorithm to evaluate the Photosystem I and Photosystem II spectral contributions to leaf chlorophyll fluorescence at physiological temperatures

Pigment composition and functional state of the thylakoid membranes during preparation of samples for pigment-protein complexes separation by nondenaturing gel electrophoresis

Deriving fluorometer-specific values of relative PSI fluorescence intensity from quenching of F 0 fluorescence in leaves of Arabidopsis thaliana and Zea mays

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