Effects of different functional strains on key metabolic pathways of methanogenesis in the domestic waste fermentation reactor

Chen, Kejin; Deng, Xiajie; Wang, Liao; Rong, He; Yang, Yinghuan; Jiang, Jiaojiao; Zhang, Chao; Yang, Shui-Wen; Li, Fan; Wang, Lei; Wang, Xiang

doi:10.1016/j.biombioe.2021.105995

Cited by 6 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A table of KEGG orthologs (KOs) and metabolic pathways were produced for each dataset. The relative abundance of functional enzymes shown in Table S1 were calculated in Microsoft Excel software [32].…”

Section: Bacterial Community Function Analysismentioning

confidence: 99%

“…In the metabolism category at KEGG level 2, three subfunctions, namely, amino acid metabolism (5.7-8.0%), carbohydrate metabolism (9.7-11.9%), and energy metabolism (3.7-4.8%), showed the highest abundances compared to those of the other subfunctions. The pathways for processing carbohydrates, proteins, and lipids into monosaccharides, amino acids, and fatty acids to generate methane are shown in Figure 5a [32]. Figure 5b,c show the relative abundances of the corresponding functional enzymes in the three pathways.…”

Section: Functional Analysis Of Bacterial Communitymentioning

confidence: 99%

“…The KEGG pathway of carbohydrate metabolism, amino acid metabolism, and energy metabolism were dominant in an anaerobic bioreactor [10,32]. Furthermore, glycine dehydrogenase (EC 1.4.4.2) and Endopeptidase La (EC 3.4.21.53), related to hydrolyzation of protein to amino acids, were more abundant than other functional enzymes [32]. Denitrification of functional genes was also abundant in a long-term operation lab-scale bioreactor [10].…”

Section: Introductionmentioning

confidence: 99%

“…The pathways for processing macromolecular organic compounds, mainly contributed by carbohydrates, proteins, and lipids, into monosaccharides, amino acids, and fatty acids involve a variety of corresponding functional enzymes. The KEGG pathway of carbohydrate metabolism, amino acid metabolism, and energy metabolism were dominant in an anaerobic bioreactor [10,32]. Furthermore, glycine dehydrogenase (EC 1.4.4.2) and Endopeptidase La (EC 3.4.21.53), related to hydrolyzation of protein to amino acids, were more abundant than other functional enzymes [32].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bacterial Community Structure and Predicted Metabolic Function of Landfilled Municipal Solid Waste in China

Han

Zhang

et al. 2022

Sustainability

View full text Add to dashboard Cite

The biodegradation behavior of municipal solid waste (MSW) depends on the diversity and metabolic function of bacterial communities, which are affected by environmental factors. However, the diversity of the bacterial communities and metabolic functions in MSW, as well as their influencing factors, remain unclear. In deep-aged MSW, the abovementioned deficiencies are more significant, and will effectively hamper landfill disposal. In this study, high-throughput sequencing was performed to examine the bacterial community structure and metabolic function from depths of 10 m to 40 m, of two large MSW landfills on the southeast coast of China. Thermotogota (1.6–32.0%), Firmicutes (44.2–77.1%), and Bacteroidota (4.0–34.3%) were the three dominant phyla among the 39 bacterial phyla identified in aged MSW samples. Bacterial genera associated with the degradation of many macromolecules, e.g., Defluviitoga, Hydrogenispora, and Lentimicrobium were abundantly detected in MSW samples, even in aged MSW. Redundancy analysis (RDA) showed that bacterial diversity in the landfills was most strongly correlated with electrical conductivity, age, and moisture content of the MSW. Tax4fun2 analysis predicted that there were abundant metabolism functions in aged MSW, especially functional enzymes (e.g., glycine dehydrogenase and cellulase) related to amino acids and cellulose degradation. This study increases our understanding of the bacterial diversity and functional characteristics in landfilled MSW.

show abstract

Section: Bacterial Community Function Analysismentioning

confidence: 99%

Section: Functional Analysis Of Bacterial Communitymentioning

confidence: 99%