Effects of different harvesting times and processing methods on the quality of cultivated Fritillaria cirrhosa D. Don

Abstract

Fritillaria cirrhosa D. Don is the major source plants of traditional Chinese medicine Fritillariae Cirrhosae Bulbus (FCB). Domestication, introduction, and cultivation is an important strategy to alleviate the shortage of endangered medicinal plants of F. cirrhosa. However, until now, the yield and quality changes of FCB in different harvest periods and drying treatments after harvest were not well understood. Therefore, in this paper, we investigated the yield and quality of cultivated F. cirrhosa at different harvest periods and postharvest processing methods. The results showed that dry weight per bulb ranged from 0.8913 to 1.4681 g and reached the highest at the wilting stage. The soluble sugar content ranged from 0.075% to 0.127% and reached the highest at the wilting stage. The content of total alkaloids ranged from 0.088% to 0.218% and reached the highest at the late‐flowering stage. The contents of peimisine, sipeimine, peimine, and peiminine were 0.01178%‐0.02615%, 0%–0.01713%, 0%–0.00745%, and 0%–0.00621% and reached the highest at the late‐flowering period, wilting period, young fruit period, and initial flowering period, respectively. For the two different postharvest processing methods, the contents of total alkaloids and the 16 main characteristic peaks did not exhibit significant differences. Still, the alkaloid contents of the oven drying after washing were slightly higher than the sun drying. In conclusion, the best harvest period is the wilting period of F. cirrhosa, and oven drying after washing is more beneficial to ensure the quality of FCB and improve productivity.

Transcriptome Analysis of CYP450 Family Members in Fritillaria cirrhosa D. Don and Profiling of Key CYP450s Related to Isosteroidal Alkaloid Biosynthesis

“…The working standard solutions for the calibration curves were created by diluting the stock solutions of imperialine (420 g/mL), peimine (413 g/mL), and peiminine (406 g/mL) with methanol. The samples were prepared for alkaloid analysis, as described by Ma [18]. The HPLC-ELSD conditions were used to determine the alkaloids, and a liquid chromatograph (Agilent 1260 Infinity, Waldbronn, Germany) equipped with an Alltech 3300 evaporative light-scattering detector was used.…”

Changes in Rhizosphere Soil Microorganisms and Metabolites during the Cultivation of Fritillaria cirrhosa

“…Fritillaria cirrhosa (FC) is a perennial herbaceous plant belonging to the genus Fritillaria in the Liliaceae family [1]. FC serves as a primary botanical reservoir of Chuan Bei Biology 2024, 13, 334 2 of 17 Mu, a traditional Chinese medicinal herb, showcasing noteworthy therapeutic efficacy in addressing diverse respiratory ailments including cough, sputum, pneumonia, bronchitis, and asthma, while also exhibiting promising potential as a pharmacological intervention for COVID-19 [2][3][4]. For thousands of years, Chuan Bei Mu has been a precious traditional Chinese medicine for moistening the lungs and relieving cough.…”

Research on image recognition of three fritillaria cirrhosa species based on deep learning

“…It is derived from the dried bulbs of the genus fritillaria (Liliaceae), such as fritillaria unibracteata Hsiao et K. C. Hsia, fritillaria cirrhosa D. Don, fritillaria przewalskii Maxim., fritillaria delavayi Franch., fritillaria taipaiensis P. Y. Li , and fritillaria unibracteata Hsiao et K. C. Hsia var. Wabuensis (S. Y. Tanget S. C. Yue) Z. D. Liu, S. Wang et S. C. Chen [2]. According to the different properties, the quality of fritillaria cirrhosa can be divided into Songbei, Qingbei, Lubei and cultivated products from high to low.…”