Effects of Different Levels of Exercise Volume on Endothelium-Dependent Vasodilation: Roles of Nitric Oxide Synthase and Heme Oxygenase

Sun, Ming; Zhong, Mingkang; Gu, Jun; Feng-lei, Qian; Gu, Jun; Chen, Hong

doi:10.1291/hypres.31.805

Cited by 58 publications

(54 citation statements)

References 40 publications

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“…All rats were subjected to the maximal running test to measure maximum aerobic velocity (MAV) (28), an index reflecting the maximum oxygen consumption (V O2max), and to determine a moderate-intensity or high-intensity training regimen based on their V O2max values. The highest velocity recorded before the animal was unable or unwilling to continue was considered the MAV.…”

Section: Methodsmentioning

confidence: 99%

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

Feng

Xun

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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He R, Feng J, Xun Q, Qin Q, Hu C. High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells. Am J Physiol Lung Cell Mol Physiol 304: L602-L612, 2013. First published February 15, 2013 doi:10.1152/ajplung.00406.2012.-A high prevalence of exercise-induced bronchoconstriction (EIB) can be found in elite athletes, but the underlying mechanisms remain elusive. Airway responsiveness, NGF and epinephrine (EPI) levels, and chromaffin cell structure in high-(HiTr) and moderate-intensity training (MoTr) rats with or without ovalbumin (OVA) sensitization were measured in a total of 120 male Sprague-Dawley rats. The expression of NGF-associated genes in rat adrenal medulla was tested. Both HiTr and OVA intervention significantly increased airway resistance to aerosolized methacholine measured by whole body plethysmography. HiTr significantly increased inflammatory reaction in the lung with a major increase in peribronchial lymphocyte infiltration, whereas OVA significantly increased the infiltration of various inflammatory cells with an over 10-fold increase in eosinophil level in bronchoalveolar lavage. Both HiTr and OVA intervention upregulated circulating NGF level and peripherin level in adrenal medulla, but downregulated phenylethanolamine N-methyl transferase level in adrenal medulla and circulating EPI level. HiTr ϩ OVA and HiTr ϩ ExhEx (exhaustive exercise) interventions significantly enhanced most of the HiTr effects. The elevated NGF level was significantly associated with neuronal conversion of adrenal medulla chromaffin cells (AMCC). The levels of p-Erk1/2, JMJD3, and Mash1 were significantly increased, but the levels of p-p38 and p-JNK were significantly decreased in adrenal medulla in HiTr and OVA rats. Injection of NGF antiserum and moderate-intensity training reversed these changes observed in HiTr and/or OVA rats. Our study suggests that NGF may play a vital role in the pathogenesis of EIB by inducing neuron transdifferentiation of AMCC via MAPK pathways and subsequently decreasing circulating EPI. exercise; asthma; lung; inflammation; NGF; neuron conversion; adrenal medulla EXERCISE-INDUCED BRONCHOCONSTRICTION (EIB) is the increase in airway obstruction with symptoms of coughing, wheezing, or dyspnea as a result of exercise (23). EIB occurs in ϳ11% of the general population without asthma (13). Inhalation of cold air and sudden changes in humidity or temperature have been associated with EIB (20). High prevalence of EIB is usually observed among endurance athletes (7), implicating that intensity of exercise may be associated with EIB.Although thermal and osmotic consequences of water loss play prominent roles in the emergence of EIB (2), more studies are needed to elucidate the underlying mechanism. Circulating epinephrine (EPI), produced by adrenal medulla chromaffin cells (AMCC), is believed to bind the adrenergic receptors of airway smooth muscles to achieve adrenergic bronchodilation, and a loss of this function could directly influence the sev...

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Section: Methodsmentioning

confidence: 99%

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

Feng

Xun

et al. 2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Among the components involved in this coronary circulation improvement are: endothelial functionf. sm 9,20,57 ; the velocity of production and oxidation of NO 6,58 ; microcirculation 9,20,57,59 ; the regression of atherosclerotic lesions 3,60,61 ; the neoformation of collateral vessels 3,60,61 ; the decrease in blood viscosity 18 and the increase in the diastolic perfusion time 59 .…”

Section: Physical Exercise and The Vascular Endotheliummentioning

confidence: 99%

Exercício físico e disfunção endotelial

Ghisi¹,

Durieux²,

Pinho³

et al. 2010

Arq. Bras. Cardiol.

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“…Îñòàííº â ñâîþ ÷åðãó çàáåç-ïå÷óºòüñÿ çíà÷íèì çðîñòàííÿì íàñîñíî¿ òà ñêîðîòëèâî¿ ôóíêö³¿ ñåðöÿ, à òàêîae âàçîäè-ëàòàö³ºþ ñóäèí ñêåëåòíèõ ìÿç³â. Ðîáî÷à ã³ïåðåì³ÿ, ÿê â³äîìî, ïîâÿçàíà ç³ çá³ëü-øåííÿì ñèíòåçó îêñèäó àçîòó â åíäîòå-ë³àëüíèõ êë³òèíàõ ñóäèí [31,36], ïðî ùî ñâ³ä÷èòü çíà÷íå ï³äâèùåííÿ àêòèâíîñò³ NO-ñèíòàçè (NOS) â ñêåëåòíèõ ìÿçàõ [30,36] ³ ñóäèíàõ òðåíîâàíèõ òâàðèí [1]. Ñèíòåç îêñèäó àçîòó ó òàêèõ òâàðèí â³äáóâàºòüñÿ íå ò³ëüêè âíàñë³äîê ïîñèëåííÿ àêòèâíîñò³ NOS, à òàêîae ³ åêñïðåñ³¿ ð³çíèõ ³çîôîðì NO-ñèíòàç [37].…”

Section: âñòóïmentioning

confidence: 99%

“…Íèí³ â³äîìî, ùî ô³çè÷í³ òðåíóâàííÿ çíèaeóþòü ãåíåðàö³þ â³ëüíèõ ðàäèêàë³â êèñíþ ì³òîõîíäð³ÿìè [33], çá³ëüøóþòü åêñïðåñ³þ åíäîãåííèõ àíòèîêñèäàíòíèõ ôåðìåíò³â òà ³íøèõ àíòèàïîïòîòè÷íèõ ïðîòå¿í³â [21], ³íäóêóþòü åôåêò ïðåêîíäèö³þâàííÿ ó äîðîñëèõ [39] ³ â³äíîâëþþòü éîãî ó ñòàðèõ òâàðèí [8]. Ïîêàçàíî ï³äâèùåííÿ ïðè òðåíóâàíí³ åêñïðåñ³¿ ð³çíèõ ³çîôåðìåíò³â NOS [25,35,36]. Ïðè òðèâàëèõ íàâàíòàaeåííÿõ âåëèêî¿ Ðîëü îêñèäó àçîòó ó ðîçâèòêó ñêîðîòëèâèõ ðåàêö³é ³íòåíñèâíîñò³ çðîñòàº åêñïðåñ³ÿ iNOS [37], çà ïîì³ðíèõ cNOS [36].…”

Section: ±11mentioning

confidence: 99%

“…Ïîêàçàíî ï³äâèùåííÿ ïðè òðåíóâàíí³ åêñïðåñ³¿ ð³çíèõ ³çîôåðìåíò³â NOS [25,35,36]. Ïðè òðèâàëèõ íàâàíòàaeåííÿõ âåëèêî¿ Ðîëü îêñèäó àçîòó ó ðîçâèòêó ñêîðîòëèâèõ ðåàêö³é ³íòåíñèâíîñò³ çðîñòàº åêñïðåñ³ÿ iNOS [37], çà ïîì³ðíèõ cNOS [36]. Çã³äíî ç íàøèìè ðåçóëüòàòàìè, àäàïòàö³ÿ äî ô³çè÷íîãî íà-âàíòàaeåííÿ ïëàâàííÿì ñóïðîâîäaeóâàëàñÿ ï³äâèùåííÿì ìåìáðàííîãî ïîòåíö³àëó ì³òîõîíäð³é, ùî ìîaeå ñâ³ä÷èòè ïðî åôåê-òèâí³ñòü ðîáîòè äèõàëüíîãî ëàíöþãà ³ ì³í³ì³çàö³þ ïðîòîííîãî âèòîêó, à òàêîae çìåíøåííÿì ÷óòëèâîñò³ ñåðäåöü äî óòâî-ðåííÿ ì³òîõîíäð³àëüíèõ ïîð ï³ä ä³ºþ Ñà 2+ , îñê³ëüêè ó òðåíîâàíèõ ùóð³â âèâ³ëüíåííÿ ì³òîõîíäð³àëüíîãî ôàêòîðà â êîðîíàðíå ðóñëî ðåºñòðóâàëîñÿ ïðè çíà÷íî á³ëüøèõ êîíöåíòðàö³ÿõ ³îí³â êàëüö³þ â ïåðôóç³éíîìó ðîç÷èí³, í³ae ó êîíòðîëüíèõ òâàðèí.…”

Section: ±11unclassified

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Untitled

2010

Fiziol Zh

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Ðîëü îêñèäó àçîòó ó ðîçâèòêó ñêîðîòëèâèõ ðåàêö³é ì³îêàðäà òðåíîâàíèõ òâàðèíÎêñèä àçîòó ñèíòåçóºòüñÿ â çíà÷íèõ ê³ëüêîñòÿõ ó òðåíîâàíèõ òâàðèí. Â³í ïîêðàùóº ÿê ïðîöåñè âàçîäèëàòàö³¿, òàê ñêîðîòëèâó òà íàñîñíó ôóíêö³þ ñåðöÿ. Çàëèøàºòüñÿ íåçÿñîâàíèì éîãî âïëèâ íà ðåàêö³¿ ñåðöÿ ó â³äïîâ³äü íà íàâàíòàaeåííÿ îáºìîì ³ êàëüö³ºì, ùî ñïîñòåð³ãàºòüñÿ ïðè òðèâàëèõ ô³çè÷íèõ íàâàíòàaeåííÿõ. Â åêñïåðèìåíòàõ íà ³çîëüîâàíîìó çà ìåòîäîì Ëàíãåíäîðôà ñåðö³ ùóð³â ïîêðàùóâàâñÿ ôóíêö³îíàëüíèé ñòàí ñåðöÿ ÿê íàñë³äîê àäàïòàö³¿ ïðîòÿãîì 4 òèae äî íàâàíòàaeåííÿ ïëàâàííÿì, ùî ïðîÿâëÿëîñü ó çá³ëüøåíí³ ñêîðîòëèâî¿ àêòèâíîñò³ ì³îêàðäà íà 20 % ³ êîðîíàðíîãî ïîòîêó (ç 12,0 ± 0,8 äî 16,0 ìë/õâ ± 1,5 ìë/õâ), çìåíøåíí³ ÷àñòîòè ñåðöåâèõ ñêîðî÷åíü, à òàêîae çá³ëüøåíí³ ôóíêö³îíàëüíèõ ðåçåðâ³â ñåðöÿ. Ïðè îäíàêîâîìó ðîçòÿãóâàíí³ ë³âîãî øëóíî÷êà ñåðöÿ òðåíîâàíèõ ùóð³â â³äïîâ³äàëè á³ëüø ïîòóaeíîþ ñèëîþ ñêîðî÷åííÿ. Âïåðøå âñòàíîâëåíî, ùî ìåìáðàííèé ïîòåíö³àë ì³òîõîíäð³é ñåðöÿ òðåíîâàíèõ ùóð³â áóâ äîñòîâ³ðíî âèùèé, í³ae ó êîíòðîëüíèõ òâàðèí (-176,5 ± 8,4 òà -156 ìÂ ± 3,5 ìÂ â³äïîâ³äíî), ùî ñâ³ä÷èòü ïðî çá³ëüøåííÿ ñïðÿaeåííÿ îêèñíîãî ôîñôîðèëþâàííÿ. Êóðñ òðåíóâàííÿ ïëàâàííÿì çàïîá³ãàâ øâèäêîìó ðîñòó ê³íöåâî-ä³àñòîë³÷íîãî òèñêó òà ïîÿâ³ àðèòì³é ïðè â³äòâîðåíí³ ìîäåë³ êàëüö³ºâîãî ïåðåâàíòàaeåííÿ (ÑàCl 2 â³ä 1,7 äî 12,5 ììîëü/ë). Ó òðåíîâàíèõ òâàðèí â³äì³÷àëè â³äêðèâàííÿ ì³òîõîíäð³àëüíèõ ïîð ïðè á³ëüø âèñîêèõ êîíöåíòðàö³ÿõ êàëüö³þ ó ïåðôóç³éíîìó ðîç÷èí³. Ïîêàçàíî, ùî àäàïòàö³ÿ äî ô³çè÷íîãî íàâàíòàaeåííÿ òà çá³ëüøåííÿ ðåçåðâ³â ñåðöÿ çóìîâëåí³ âïëèâîì îêñèäó àçîòó, áëîêàäà ñèíòåçó ÿêîãî çà äîïîìîãîþ L-NÀÌÅ (10 -4 ìîëü/ë) óñóâàëà âêàçàí³ àäàïòàö³éí³ çì³íè. Êëþ÷îâ³ ñëîâà: îêñèä àçîòó, ³çîëüîâàíå ñåðöå, ô³çè÷íå òðåíóâàííÿ ïëàâàííÿì, êðèâà Ôðàíêà Ñòàðë³íãà, ïåðåâàíòàaeåííÿ êàëüö³ºì.

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Effects of Different Levels of Exercise Volume on Endothelium-Dependent Vasodilation: Roles of Nitric Oxide Synthase and Heme Oxygenase

Cited by 58 publications

References 40 publications

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

High-intensity training induces EIB in rats through neuron transdifferentiation of adrenal medulla chromaffin cells

Exercício físico e disfunção endotelial

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