Effects of Different Media on Micropropagation and Rooting of Myrtle (Myrtus communis L.) in In Vitro Conditions

Şimşek, Özhan; Biçen, Belgin; Dönmez, Dicle; Kaçar, Yıldız Aka

doi:10.25125/agriculture-journal-ijoear-oct-2017-22

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…There were some differences in the propagation and rooting parameters of the present genotypes. Previous tissue culture studies in myrtle plants also revealed different outcomes for myrtle genotypes in the same media ( Şimşek et al, 2017 ; Aka Kaçar et al, 2020 ).…”

Section: Discussionmentioning

confidence: 97%

In vitro and ex vitro propagation of Turkish myrtles through conventional and plantform bioreactor systems

Şimşek,

Dönmez,

Sarıdaş

et al. 2023

PeerJ

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The myrtle (Myrtus communis) plant naturally grows in the temperate Mediterranean and subtropical regions and is used for various purposes; thus, it is among the promising species of horticultural crops. This study aimed to evaluate and compare the performance of different propagation systems, including rooting, solid media propagation, rooting, and with the Plantform bioreactor system, in achieving healthy and rapid growth of four myrtle genotypes with diverse genetic origins and well-regional adaptation. The selection of myrtle genotypes with distinct genetic backgrounds and proven adaptability to specific regions allowed for a comprehensive assessment of the propagation systems under investigation. Present findings proved that the Plantform system, the new-generation tissue culture system, was quite successful in micropropagation and rooting myrtle genotypes. We succeeded in vitro micropropagation and rooting of diverse wild myrtle genotypes, enabling year-round propagation without reliance on specific seasons or environmental conditions. The process involved initiating cultures from explants and multiplying them through shoot proliferation in a controlled environment. This contributes to sustainable plant propagation, preserving and utilizing genetic resources for conservation and agriculture.

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Section: Discussionmentioning

confidence: 97%

In vitro and ex vitro propagation of Turkish myrtles through conventional and plantform bioreactor systems

Şimşek,

Dönmez,

Sarıdaş

et al. 2023

PeerJ

Self Cite

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“…The pH was adjusted to 5.7 by adding 30 g/l sucrose and 6.5 g/l agar to the medium. The medium was poured into culture vessels and sterilized in an autoclave at 121 °C and 1.05 atmosphere pressure (Simsek et al, 2017). Nodal explants were cultured in sterile nutrient medium.…”

Section: Nutrient Mediummentioning

confidence: 99%

Micropropagation of autochthonous olive varieties from Türkiye

CIFTCI,

SAKAR,

ERCISLI

et al. 2023

Not Bot Horti Agrobo

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Olive (Olea europaea L.) is one of the oldest cultivated fruit species in the world. Fruits and oils of autocthonous olive varieties (native Turkish olive varieties) with unique sensory properties (taste, smell and aroma) gain importance recently. Particularly olive oil companies looking for varieties that have distinct taste, smell and aroma in their oil. Propagation of olive varieties by cuttings and grafting is very difficult and expensive, therefore it is important to find solution for easy and mass propagation. Micropropagation is particularly beneficial to propagate plants that are difficult to reproduce conventionally or to ensure virus-free plants or plants with particular qualities. In this study, in vitro micropropagation success of two autocthonous olive varieties (‘Mavi’ and ‘Guleki’) grown in the origin center of olive, Southeast Anatolia, Türkiye was investigated. Both varieties, have distinct smell, taste and aroma and are difficult to root by cuttings. The effects of three different medium OM (Olive Medium), WPM (Woody Plant Medium) and DKW (Driver-Kuniyuki Walnut Medium) and two different growth regulators BAP (6-Benzylaminopurine) and Zeatin on shoot induction (proliferation) in the in vitro micropropagation of the olive varieties were examined. Obtained shoots were later subjected to in vitro rooting and acclimatization. The highest proliferation efficiency and shoot length for both varieties were obtained with the use of 1 mg Zeatin+0.1 mg GAɜ hormone combinations on OM medium. ‘Mavi’ variety formed more roots compared to ‘Guleki’ (3.33 vs. 2.75 per shoots), and gave the highest rooting rate of 74.33% with 2 mg IBA (Indole Butyric Acid) treatment on ½ OM medium. In terms of rooting rate, Guleki gave the highest rooting (100%) on medium containing ½ OM, 0.2 mg GA3 and 4 mg IBA. 60% and 75% of the micropropagated plants of ‘Mavi’ and ‘Guleki’ varieties adapted well to the external conditions.

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“…Its leaves and fruit are rich in phenol and flavonoids. Its phytochemicals and high antioxidant capacity make myrtle plant more important day by day (Şimşek et al, 2017;Hennia et al, 2018;Aka Kaçar et al, 2020). In this study, the 'C-1' genotype with white fruits and the 'C-2' genotype with black fruits were used.…”

Section: Introductionmentioning

confidence: 99%

Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes

Açar¹,

Kaçar²

2022

Turkish JAF Sci.Tech.

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The Myrtle plant (Myrtus communis L.) is a plant species of the Myrtaceae family and a member of the maquis community, which naturally spreads in Mediterranean regions. Being resistant to arid conditions, the ecological distribution areas of the myrtle plant have been allowed to expand. The myrtle plant has been used of medicinal and aromatic plants, having interesting and beautiful flowers, and rich nutrient content of the fruit in terms of valuable phytochemicals, in particular, the nutritional content of its fruits and valuable metabolites have allowed the myrtle plant to be among the healthier foods. Antioxidant activity, which neutralizes reactive oxygen species (ROS), which causes many medical problems, is one of the most important features of the myrtle plant. Investigation of the biosynthesis of anthocyanin, which leads to antioxidant activity, and determination of the biosynthesis in different tissues and genotypes is important, especially in the development of production activities. Furthermore, this study aimed to investigate the anthocyanin biosynthesis in different genotypes with white and black fruits and various tissues of genotypes. For this purpose, the expression levels of CHS, CHI, F3H and PAL genes, which are involved in the anthocyanin biosynthesis pathway, were determined by qRT-PCR. In the study, it was determined that there was an increase in the level of genes related to the biosynthesis of anthocyanin in the leaf and fruit tissues of the genotype with white fruits. It was determined that the expression level of genes related to the biosynthesis of anthocyanin was observed to be higher in the leaf and fruit tissues of the genotype with black fruits, and the highest gene expression level was found in black fruits. It was observed that anthocyanin biosynthesis was synthesized in different tissues of the plant, and anthocyanin biosynthesis was higher in fruits compared to leaves.

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Effects of Different Media on Micropropagation and Rooting of Myrtle (Myrtus communis L.) in In Vitro Conditions

Cited by 8 publications

References 12 publications

In vitro and ex vitro propagation of Turkish myrtles through conventional and plantform bioreactor systems

In vitro and ex vitro propagation of Turkish myrtles through conventional and plantform bioreactor systems

Micropropagation of autochthonous olive varieties from Türkiye

Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes

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