Effects of different modified diets on growth, digestive enzyme activities and muscle compositions in juvenile Siamese fighting fish (Betta splendens Regan, 1910)

Abstract

Havforskningsinstituttets institusjonelle arkiv Brage IMR - Institutional repository of the Institute of Marine Research b r a g e i m rDette er forfatters siste versjon av den fagfellevurderte artikkelen, vanligvis omtalt som postprint. I Brage IMR er denne artikkelen ikke publisert med forlagets layout fordi forlaget ikke tillater dette. Du finner lenke til forlagets versjon i Brage-posten. Det anbefales at referanser til artikkelen hentes fra forlagets side. Ved lenking til artikkelen skal det lenkes til post i Brage IMR, ikke direkte til pdf-fil.This is the author's last version of the article after peer review and is not the publisher's version, usually referred to as postprint. You will find a link to the publisher's version in Brage IMR. It is recommended that you obtain the references from the publisher's site.Linking to the article should be to the Brage-record, not probiotic-supplemented and carbohydrases-supplemented diets) were studied on digestive 25 enzyme specific activities and growth performance quality of juvenile Siamese fighting fish 26 (Betta splendens Regan, 1910) during 2 weeks of critical and intensive rearing period. The 27 modified procedures did not change biochemical compositions and gross energy of diets, but 28 generally resulted in relatively higher in vitro digestibilities of protein and carbohydrate and 29 fish survival rate, albeit insignificant. Only gamma irradiation significantly increased in vitro 30 protein digestibility of the diet, and microwave irradiation increased starch gelatinization and 31 water solubility (P < 0.05). Fish fed microwave-treated diet showed highest values in all 32 studied growth indicators and digestive enzyme specific activities (except lipase), with 33 significantly higher amylase specific activity and activity ratio of amylase to trypsin (A/T 34 ratio). Correlation analysis indicated significant relationships (P < 0.05) among the levels of 35 total protease, amylase and trypsin, and between SGR and A/T ratio. Muscle and body 36 compositions of juveniles fed on microwave-or gamma-irradiated diets were similar to the 37 control, while the juveniles fed on probiotic-or carbohydrases-supplemented diets showed 38 lower protein depositions (P < 0.05). Similar levels of RNA, RNA/Protein ratio, and 39Protein/Lipid ratio in body and muscle in all dietary groups fed ad libitum suggested that the 40 improved growth performance in juvenile Siamese fighting fish fed on microwave-irradiated 41 diet may not be only due to improved physicochemical properties of the diet but also 42 improved fish consumption rate. 43 44

Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia

“…Digestive enzymes Intestine sample preparation and digestive enzymes analyses were performed as described by Thongprajukaew et al (2011). Intestine samples (n = 6/treatment) were diluted, homogenized, centrifuged and the supernatant used for analyse.…”

Faecal characteristics as markers of Chelonia mydas feeding

“…We have previously suggested that feed utilization efficiency in aquatic animals is typically studied through digestive enzymes extracted from the alimentary tracts 3 . In higher animals, faecal analysis can be used for monitoring feed quality in domestic livestock 4 , and for identifying sex and species of organisms present in the wild 5 .…”

Effects of red monascal rice supplementation on growth, digestive function and oocyte maturation in Siamese fighting fish (Betta splendens Regan, 1910)

“…Specified optimal conditions for studying digestive enzyme activities in Siamese fighting fish were used as described in Thongprajukaew et al (2011). Amylase activity was determined based on Areekijseree et al (2004) using starch solution as a substrate.…”

“…Moreover, supplementation of aquaculture feed with red monascal rice for rearing juvenile Siamese fighting fish has been reported (Thongprajukaew et al, 2011). Safety evaluation of red monascal rice for use as a food supplement has been studied in chickens , rats (Chan and Shiao, 2007;Kumari et al, 2009) and rabbits (Wei et al, 2003).…”