Effects of different periods of renal ischemia on liver as a remote organ

Abstract

AIM:To assess the hepatic changes after induction of different periods of renal ischemia. METHODS:Rats were subjected to either sham operation or ischemia (30, 45 and 60 min) followed by 60 min reperfusion. Liver and renal functional indices were measured. Hepatic glutathione (GSH) and ferric reducing antioxidant power levels and the concentration of interleukin (IL)-10 and tumor necrosis factor (TNF-α) were evaluated. Portions of liver and kidney tissues were fixed for histological evaluation. RESULTS:Forty-five minutes renal ischemia followed by 60 min reperfusion caused significant changes in liver structure and a significant reduction in renal function. These rats showed a significant decrease in liver GSH, as well as a significant increase in TNF-α and IL-10 concentrations. These results demonstrated that renal ischemia caused changes in liver histology, function, oxidative stress and inflammatory status, which led to a reduction in hepatic antioxidant capacity. With 30 min ischemia, the magnitude of these changes was less than those with 45 or 60 min ischemia. CONCLUSION:A minimum of 45 min ischemia is needed to study the effects of renal injury on the liver as a remote organ.

Gender difference in the development of cardiac lesions following acute ischemic-reperfusion renal injury in albino rats

“…These results are in line with Mount et al (2012) and Chen et al (2012). Furthermore, Kadkhodaee et al (2009) reported that the rise of renal injury markers was dependent on the renal ischemia time, and Jung et al (2009) found the increase in renal injury markers reached the peak 48 hours after the renal I/R injury. On the other hand, Vinodini et al (2009) reported no change in serum urea level after renal I/R; this controversy is due to protocol differences as the rats underwent unilateral I/R, and the reperfusion period was only 90 minutes.…”

Effects of Aqueous Extract of Purslane (Portulaca oleracea) on Hepatic Enzymes in Two Models of Renal Ischemia-Reperfusion Injury in Rats

“…Previous studies reported that renal IR could disturb liver function and morphology [9,10]. Kadkhodaee et al suggested that a minimum of 45 minutes ischemia period is necessary to produce the liver damage as a remote organ [10]. Wang et al showed that 1 hour ischemia and 4 or 8 hours reperfusion of the kidney increased the serum ALT and AST which seemed to be due to the hepatic cell membrane damage [9].…”

“…Previous studies reported that renal IR could disturb liver function and morphology [9,10]. Kadkhodaee et al suggested that a minimum of 45 minutes ischemia period is necessary to produce the liver damage as a remote organ [10].…”

Possible Protective Effect of Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) Against the Remote Liver Injury Induced by Renal Ischemia Reperfusion in Male Albino Rats

“…functional derangements and cell death ultimately [9]. This complex pathophysiology resulting from a number of contributing factors, so it is difficult to achieve effective treatment or protection by targeting only individual mediators or mechanisms despite the efforts made to deal with this problem [10].…”

“…The increasing mortality rate in cases with acute renal injury necessitates designing strategies to assess the effect of renal injury on distant organs [11]. Therefore, several studies [8,[1][2][3][4][5][6][7][8][9][10][11][12][13][14] tried to protect liver from remote injury induced by renal IRI for the prevention and attenuation of acute renal injury related morbidity.…”