Effects of different physical factors on osteogenic differentiation

Peng, Li; Wu, Fanzi; Cao, Mengjiao; Li, Mengxin; Cui, Jingyao; Liu, Lijia; Zhao, Yun; Yang, Jing

doi:10.1016/j.biochi.2022.10.020

Cited by 4 publications

(2 citation statements)

References 157 publications

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“…The successful clinical outcomes of ES in bone healing strategies encouraged the scientific community to try to understand its underlying mechanisms at both the cellular and molecular levels. ES has been previously applied to enhance the osteogenic differentiation of MSCs in vitro 17 , 39 , 40 . However, the cellular processes/signaling pathways by which ES regulates osteogenesis are still poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Direct coupled electrical stimulation towards improved osteogenic differentiation of human mesenchymal stem/stromal cells: a comparative study of different protocols

Silva,

Meneses,

Garrudo

et al. 2024

Sci Rep

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Electrical stimulation (ES) has been described as a promising tool for bone tissue engineering, being known to promote vital cellular processes such as cell proliferation, migration, and differentiation. Despite the high variability of applied protocol parameters, direct coupled electric fields have been successfully applied to promote osteogenic and osteoinductive processes in vitro and in vivo. Our work aims to study the viability, proliferation, and osteogenic differentiation of human bone marrow-derived mesenchymal stem/stromal cells when subjected to five different ES protocols. The protocols were specifically selected to understand the biological effects of different parts of the generated waveform for typical direct-coupled stimuli. In vitro culture studies evidenced variations in cell responses with different electric field magnitudes (numerically predicted) and exposure protocols, mainly regarding tissue mineralization (calcium contents) and osteogenic marker gene expression while maintaining high cell viability and regular morphology. Overall, our results highlight the importance of numerical guided experiments to optimize ES parameters towards improved in vitro osteogenesis protocols.

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Section: Discussionmentioning

confidence: 99%

Direct coupled electrical stimulation towards improved osteogenic differentiation of human mesenchymal stem/stromal cells: a comparative study of different protocols

Silva,

Meneses,

Garrudo

et al. 2024

Sci Rep

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“…Bone repair is a complex process, which involves the recruitment, proliferation, and differentiation of BMSCs as well as the subsequent osteogenic mineralization of osteoblasts. The transformation of osteoblasts into fully mature cells also goes through three stages, including cell proliferation, matrix maturation, and matrix mineralization. − Since a specific bioactive element may only affect one or several stages in the whole bone repair process, it is of great significance to explore the effective stage and the promotive mechanism of different bioactive elements for research and application of multiple bioactive elements in synergistically improving the bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Vanadium-Doped Mesoporous Bioactive Glass Promotes Osteogenic Differentiation of rBMSCs via the WNT/β-Catenin Signaling Pathway

Cong,

Zhang,

et al. 2023

ACS Appl. Bio Mater.

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Pentavalent vanadium [V(V)] has been studied as bioactive ions to improve the bone defect repair; however, its osteogenic promotion mechanism is still not fully understood so far. In this study, a V-doped mesoporous bioactive glass (V-MBG) was prepared, and its effects on osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) and potential signaling pathways were investigated. The physicochemical characterization revealed that the incorporation of V slightly reduced the specific surface area and increased the mesoporous pore size, and the abundant mesopores of V-MBG were beneficial to the sustained dissolution of V(V) ions as well as calcium, silicon, and phosphorus ions. Cell proliferation results indicated that the high dilution ratio (>16) V-MBG extract markedly promoted the proliferation of rBMSCs compared with the control group and the same dilution ratio MBG extract. Compared with the same dilution ratio MBG extract, diluted V-MBG extracts markedly promoted the secretion of alkaline phosphatase (ALP) and osteocalcin (OCN) protein at day 7 but insignificantly stimulated the runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor (VEGF) protein synthesis. In depth, the diluted V-MBG extracts remarkably up-regulated the expression of WNT/β-catenin pathway direct target genes, including WNT3a, β-catenin, and AXIN2 genes in contrast to the same dilution ratio MBG extracts, suggesting that the released V(V) ions might promote osteogenic differentiation of rBMSCs via the WNT/β-catenin signaling pathway.

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The Role of PEMFs on Bone Healing: An In Vitro Study

Caliogna

Bina

Brancato

et al. 2022

IJMS

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Bone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their action. Specifically, we evaluated the effects of PEMFs both on human adipose mesenchymal stem cells (hASCs) and on primary human osteoblasts (hOBs) by testing gene and protein expression of early bone markers (on hASCs) and the synthesis of late bone-specific proteins (on hOBs) as markers of bone remodeling. Our results indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells. Understanding the role of PEMFs on bone regenerative processes provides important details for their clinical application.

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Effects of different physical factors on osteogenic differentiation

Cited by 4 publications

References 157 publications

Direct coupled electrical stimulation towards improved osteogenic differentiation of human mesenchymal stem/stromal cells: a comparative study of different protocols

Direct coupled electrical stimulation towards improved osteogenic differentiation of human mesenchymal stem/stromal cells: a comparative study of different protocols

Vanadium-Doped Mesoporous Bioactive Glass Promotes Osteogenic Differentiation of rBMSCs via the WNT/β-Catenin Signaling Pathway

The Role of PEMFs on Bone Healing: An In Vitro Study

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