Effects of different soil weights, storage times and extraction methods on soil phospholipid fatty acid analyses

Wu, Yuping; Ding, Na; Wang, Gang; Xu, Jianming; Wu, Jianjun; Brookes, Philip C.

doi:10.1016/j.geoderma.2009.02.003

Cited by 124 publications

(57 citation statements)

References 26 publications

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“…Tubes were sealed and placed in an 25°C incubator. The tubes were randomly taken out from the incubator after 1, 3, 7, 14, 21, 28 and 35 days for the soil enzyme activity analysis and microbial community analysis (PLFA) [11] .…”

Section: Soil Enzyme Activity Analysismentioning

confidence: 99%

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Degradation of S-metolachlor and its Effects on Soil Enzymes and Microbial Communities in Vegetable Field Soil

Huang¹,

Cui²,

Zhou³

et al. 2017

JRST

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S-metolachlor is a promising alternative to metolachlor. However, the extensive use of S-metolachlor as herbicide in vegetable fields in China has caused concerns about its environmental fate. Here we investigated the effects of temperature, relative humidity (RH) and PH on the degradation rate of S-metolachlor in vegetable soil. The degradation rates of S-metolachlor increased with increasing temperatures and RH and either acidic or basic PH facilitates S-metolachlor degradation. The degradation of S-metolachlor under these conditions followed the first-order kinetics resulting in the half-lives (T-1/2) ranging from 12.18 d to 70.71 d at 5-35°C, and 27.28 d to 53.72 d at RH 30-90%; and 29.62-19.69 d at pH 6-8. Stronger response of soil enzymes including catalase, dehydrogenase, urease and cellulase to S-metolachlor was detected in soil with high organic matter. PLFA profiles showed that, totally, the microbe populations including actinomycetes, fungi and bacteria increased gradually in the first 14 days after the treatment and decreased from 14d to 28d after the treatment. All the S-metolachlor treatments caused the increase of aerobe and anaerobe. High S-metolachlor concentration, 13.9 mg/kg, could cause significant variation at the first 2 weeks, stimulating growth of the entire soil microbial community. These findings might have practical implications for the fate of S-metolachlor residue in vegetable fields. Environmental factors, especially temperature, relative humidity and pH should be considered in combination with the appropriate application dose of S-metolachlor for achieving satisfactory weed-control efficacy, reducing runoff, and minimizing effects on environmental quality.

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Section: Soil Enzyme Activity Analysismentioning

confidence: 99%

“…Microbial community analysis was determined by Phospholipid Fatty Acid (PLFA) [15,16,11]. Briefly, 5 g freeze-dried soil was extracted with a one-phase mixture of CHCl 3 /MeOH/citric acid buffer (0.15 M, pH 4)(1:2:0.8, v/v/v).…”

Section: Soil Microbial Community Analysismentioning

confidence: 99%

Degradation of S-metolachlor and its Effects on Soil Enzymes and Microbial Communities in Vegetable Field Soil

Huang¹,

Cui²,

Zhou³

et al. 2017

JRST

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“…The Psammaquent and Plinthudult were collected from the Zhejiang province of China, as composites of individual cores (0-10 cm depth), as described by Wu et al (2009a). Obvious vegetation, roots and stones were removed, and the soils then air-dried and sieved < 2 mm.…”

Section: Soils and Biocharmentioning

confidence: 99%

Impact of wheat straw biochar on yield of rice and some properties of Psammaquent and Plinthudult

Muhammad

Aziz

Brookes

et al. 2017

J. Soil Sci. Plant Nutr.

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Agricultural organic matter is a major component of organic waste on earth that significantly contributes in environmental pollution. The conversion of organic waste into biochar and addition to soil is the recommended strategy to reduce the negative environmental effects of organic waste and to increase the soil fertility. Therefore, in this study, we evaluated the changes in nutrient concentrations, rice yield and microbial community structure in a Psammaquent and Plinthudult at harvest following incorporation of biochar derived from wheat straw. Wheat straw biochar generated at 300-500 ˚C under oxygen limited conditions was applied, in a greenhouse experiment to a Psammaquent and Plinthudult at a rate of 3% weight/weight (w/w). The biochar addition to both types of soil significantly increased the soil pH from 4.2 to 6.2 and 4.7 to 6.7, total nitrogen by 135 and 37%, and organic carbon by 90 and 80%, in the Psammaquent and Plinthudult, respectively as compared to the respective controls.The dissolved organic nitrogen (DON) was decreased by 24 and 15% and dissolved organic carbon (DOC) by 40 and 44% in Psammaquent and Plinthudult, respectively. The biochar decreased the concentrations of K leached by 24%, B by 25%, Cu by 80%, Mn by 37% and Zn by 33% in the Psammaquent and B by 50%, Cu by 60%, Fe by 43%, Mn by 69% and Zn by 83% in the Plinthudult as compared to the controls. The wheat straw biochar addition to two soils increased the accumulation of Mn, Mo, Na and Zn in both rice straw and grain and decreased the leaching of nutrients, organic carbon and nitrogen at rice harvest and increased the leaching of Ca, Na, and Mg in both Psammaquent and Plinthudult. The biochar increased the pH, TOC and nitrogen in both soils. The biochar significantly changed the PLFA concentration indicating different microbial community pattern in soils growing rice compared to their controls. Thus, the results indicated that wheat straw biochar increased the productivity of rice in the Plinthudult and Psammaquent.

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“…Using the freeze-dried soil samples, PLFAs were extracted and identified according to Wu et al (2009). Lipids were extracted using a singlephase chloroform-methanol-citrate buffer system.…”

Section: Phospholipid Fatty Acid Analysis (Plfas)mentioning

confidence: 99%

Effect of biochar application on microbial biomass and enzymatic activities in degraded red soil

Demisie¹,

Zhang²

2015

Afr. J. Agric. Res.

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To assess biochar effect on soil microbial biomass, community and enzymatic activities, degraded acidic soil was amended with three different rates (0.5, 1.0 and 2.0%) of oak wood biochar (W 0.5 , W 1.0 and W 2.0 ) and bamboo biochar (B 0.5 , B 1.0 and B 2.0 ), with control as 0%. The soil and the biochar were mixed thoroughly, wetted and incubated at a constant temperature of 25°C. The amended soil properties were evaluated after the 1st, 8th and 16th weeks of the incubation. It was found that soil pH, total organic carbon (TOC) and urease increased significantly with increasing biochar rate while the activity of acid phosphatase decreased, the reason can be the inverse correlation of this enzyme with soil pH. TOC had positive correlation with urease. The β-glucosidase correlated positively with dissolved organic carbon (DOC) and negatively with C/N, suggesting that mineralization of organic matter provides substrates for this enzyme. The highest microbial biomass C as well as total Phospholipid fatty acid analysis (PLFA) was observed at the lowest rates, particularly the treatment of W0.5 had higher relative abundance of soil bacteria, fungi and gram-positive bacteria. Our results suggest that biochar application improve the fertility of degraded red soil by increasing soil pH, TOC and DOC which, in turn, enhance soil enzymes, microbial biomass and community.

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Effects of different soil weights, storage times and extraction methods on soil phospholipid fatty acid analyses

Cited by 124 publications

References 26 publications

Degradation of S-metolachlor and its Effects on Soil Enzymes and Microbial Communities in Vegetable Field Soil

Degradation of S-metolachlor and its Effects on Soil Enzymes and Microbial Communities in Vegetable Field Soil

Impact of wheat straw biochar on yield of rice and some properties of Psammaquent and Plinthudult

Effect of biochar application on microbial biomass and enzymatic activities in degraded red soil

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