Effects of different sources of diet on the growth, survival, biochemical composition and physiological metabolism of clam (
            <i>Cyclina sinensis</i>
            )

Liao, Xiaoting; Sun, Zepeng; Cui, Zhenquan; Yan, Susu; Fan, Sishao; Xia, Qing; Shi, Junjie; Ge, Hongxing; Liu, Meimei; Dong, Zhiguo

doi:10.1111/are.15886

Cited by 7 publications

(5 citation statements)

References 46 publications

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“…Most aquatic animals cannot grow without adequate light and food, such as Oreochromis mossambicus , Litopenaeus vannamei , Scylla paramamosain and Icralurus puncrarus (Guo et al, 2013; Konkal & Ganesh, 2018; Liao et al, 2022; Lr & Robinson, 2005; Zhao et al, 2015). Improper light can cause aquatic organisms stunting and even death (Woolley et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression analysis of CDK genes during selective and wild populations of juvenile Meretrix meretrix

Chen

et al. 2022

Aquaculture Research

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The saltwater hard clam Meretrix meretrix is an important commercially bivalve in China. Therefore, identification of key genes involved in growth regulation are the primary prerequisites for further development of phenotype-selective breeding of the clams and understanding molecular mechanisms of growth regulation. In the present study, we cloned the full-length cDNA sequences of the growth-related genes cyclindependent kinase 1 and 7 in M. meretrix (MmCDK1 and MmCDK7) and then investi-

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression analysis of CDK genes during selective and wild populations of juvenile Meretrix meretrix

Chen

et al. 2022

Aquaculture Research

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“…However, detailed findings on the sex determination and differentiation of C. sinensis have not been reported. Understanding the sex determination and differentiation of C. sinensis is important for the reproduction and breeding of clams (Wei et al, 2020;Dong et al, 2021;Liao et al, 2022). In the present study, the full-length cDNAs of DMRT3 and FOXL2 were cloned first.…”

Section: Introductionmentioning

confidence: 87%

Characterization, expression profiling, and estradiol response analysis of DMRT3 and FOXL2 in clam Cyclina sinensis

Yan¹,

Xu²,

Xie³

et al. 2023

Front. Mar. Sci.

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The clam Cyclina sinensis is one of the important economical aquaculture shellfish in China. However, the mechanisms of sex determination and differentiation in C. sinensis have not been fully studied. In this study, full-length cDNAs of DMRT3 and FOXL2 were cloned and functionally characterized. The ORF region of CsDMRT3 consists of 1137 nucleotides, which encode 378 amino acids contains a conserved DM domain of DMRT family. The ORF region of CsFOXL2 is 1245 bp, encodes 414 amino acids, and contains a conserved FH domain. Tissue-specific expression results showed that the higher expression level of CsDMRT3 and CsFOXL2 was found in the ovary and testis of C. sinensis. The expression levels of CsDMRT3 and CsFOXL2 also peaked at the maturation stage of male and female gonadal development, respectively. Moreover, the expression levels of CsDMRT3 and CsFOXL2 were significantly higher in the trochophore and D-larval stages than in other stages. The transcript levels of CsDMRT3 reached the highest level at 11 months of age, while the CsFOXL2 reached the highest level at 7 months of age. In estradiol-treated experiments, the expression levels of CsDMRT3 and CsFOXL2 in the gonads were highest at 5 µg/L estradiol treatment, and histologically, it was observed that the oocytes diameters became larger with increasing estradiol concentration. These results suggest that CsDMRT3 and CsFOXL2 play an important role in gonadal development and sex differentiation of C. sinensis.

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“…Due to the advantages of strong resistance to adversity, tasty meat and high culture yield, the clam has become an ideal cultured shellfish with huge production and sales in China (Zhang, 2014;Shi et al, 2019). As a typical burial-type economic shellfish, C. sinensis, is good bioindicator for coastal pollution investigations and has been widely cultivated and proliferated in China (Wei et al, 2020;Ge et al, 2021;Liao et al, 2022). Therefore, C. sinensis could be used as a model species to understand the potential toxicity effects of marine bivalves exposed to residual chlorine.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

Wang¹,

Ren²,

Li³

et al. 2023

Front. Mar. Sci.

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Residual chlorine is a common by-product of warm drainage in coastal nuclear power plants. when accumulating to some limit, it may threaten marine ecosystem especially for benthic clam. However, there are few studies on the molecular mechanisms related to immunity and antioxidant of residual chlorine stress on clams. In this study, the clam (Cyclina sinensis) was exposed for 96 h at different concentrations (0, 50, 100, 150, 200, 250, 300, 350, 400, 450 and 500 mg/L) of residual chlorine to observe its mortality, measure the activity of antioxidant and immune-related enzymes, and analyses the gene expression level in the hepatopancreas by using the transcriptome sequencing. The results showed that the mortality rate increased with the increase of stress time and concentration, and the mortality rate in the 400, 450 and 500 mg/L groups reached 100% at 96 h. The tolerance to residual chlorine of C. sinensis decreased with the increase of chlorine dioxide concentration, and the LC50 of 96 h was 217.6 mg/L by linear regression method. After residual chlorine stress, the activity of antioxidant-related enzymes (T-AOC and SOD) in the hepatopancreas showed a trend of first increase and then decrease with the extension of stress time. The immune-related enzyme activities of AKP and LZM showed a downward trend between 0 and 96 h, while the ACP enzyme activity showed a trend of first rising and then decreasing. Transcriptome analysis showed that residual chlorine stress significantly changed the expression levels of immune-related molecules associated with signal transduction, prophenoloxidase cascade, cell apoptosis and pattern recognition protein/receptor. Moreover, glutathione S-transferase (GST), heat shock protein (HSP) and other antioxidant-related genes were significantly affected under residual chlorine stress. This study provided valuable information for understanding the effects of residual chlorine stress on survival, physiological metabolism and molecular mechanisms of immune and antioxidant functions of C. sinensis.

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Effects of different sources of diet on the growth, survival, biochemical composition and physiological metabolism of clam ( Cyclina sinensis )

Cited by 7 publications

References 46 publications

Molecular cloning and expression analysis of CDK genes during selective and wild populations of juvenile Meretrix meretrix

Molecular cloning and expression analysis of CDK genes during selective and wild populations of juvenile Meretrix meretrix

Characterization, expression profiling, and estradiol response analysis of DMRT3 and FOXL2 in clam Cyclina sinensis

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

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