Effects of Different Therapeutic Ultrasound Intensities on Fracture Healing in Rats

A mechano-regulatory model for bone healing predictions under the influence of ultrasound

“…These findings are in agreement with previous animal studies on a sheep osteotomy which report that transosseous ultrasound application leads to an increase in bone matrix density and accelerated healing time [43]. Increased levels of bone density due to ultrasound are also reported in [27,51].…”

“…In addition they also found higher amounts of cartilage at weeks 2-4 post-fracture in the LIPUS group suggesting that the earlier callus formation may be attributed to enhanced endochondral ossification. In a later work [27] aiming at the investigation of the effect of different LIPUS intensities on fracture healing it was found that low-density bone volume fraction was significantly higher in the group treated with LIPUS at ISATA = 30mW/cm 2 than in the control group. LIPUS at higher intensity was found not to further accelerate bone healing.…”

Low Intensity Pulsed Ultrasound Enhanced Mesenchymal Stem Cell Recruitment through Stromal Derived Factor-1 Signaling in Fracture Healing

“…Low intensity pulsed ultrasound (LIPUS) has been reported to be effective in promoting fracture healing in both animal models and clinical trials [13]–[16]. In brief, the beneficial effects of LIPUS on fracture healing include the decrease in healing time at the tissue level, and the increase in the cellular responses including osteogensis-related gene expression [17], protein synthesis and cell proliferation [18].…”

Short-Term Effect of Low-Intensity Pulsed Ultrasound on an Ex-Vivo 3-D Tooth Culture

“…LIPUS has been shown to enhance periosteal cell expansion (Leung et al 2004), stimulate BMSC expansion and differentiation into chondrocytes (Cui et al 2006;Ebisawa et al 2004), and increase matrix production and proliferation of the intervertebral disc cell culture (Iwashina et al 2006). Although many experimental studies and clinical trials support that LIPUS enhances healing of fractured bone by enhancing matrix formation (Fung et al 2012;Li et al 2012), some clinical trials showed controversial results or no stimulatory effect of LIPUS in healing fractured bone (Bashardoust Tajali et al 2012). Although it has been hypothesized that LIPUS application angle is important to produce stimulatory effect on bone matrix formation at the fractured bone (Chung et al 2011), it is worth performing further research to uncover the genetic underpinning of the stimulatory or inhibitory effects of LIPUS on hard tissue matrix formation.…”