Effects of different types and doses of whey protein on the physiological and intestinal flora in D-galactose induced aging mice

Abstract

The elderly usually suffer from many diseases. Improving the quality of life of the elderly is an urgent social issue. In this present study, D-galactose treated aging mice models were used to reveal the effects of different animal sources and different doses of whey protein (WP) on the immune indexes organs and intestinal flora. A total of 9 groups were set up, including normal control (NC), negative control (NS), positive control (Vc), low-, medium- and high-doses of cow WP intervention groups (CL, CM and CH for short, correspondingly) and low-, medium- and high-doses of goat WP intervention groups (GL, GM and GH for short, correspondingly). The body weight gain, thymus/body weight ratio, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, spleen immunoglobulins G (IgG), spleen interleukin-2 (IL-2) and spleen interleukin-2 (IL-6) were measured. Then, the intestinal contents were collected, and 16s genes of intestinal bacteria were sequenced to reveal the changes in bacterial flora structure. WP intervention significantly increased the weight gain, thymus/body ratio and SOD activity, but decrease the content of MDA. WP intervention increased some immune indicators. All the WP treated aging mice showed similar values of physiological indexes to that of the Vc group, even better. The relative abundance of Lactobacillus and Stenotrophomonas was increased and decreased, respectively, by both cow and goat WP. Lactobacillus may be involved in regulating the functional repair of organisms. In contrast, Stenotrophomonas might play a negative role in the immune and antioxidant capacity of the body. Combining physiological indicators and intestinal flora structure, low-concentration WP for cow and goat might be optimal for aging models.

Whey and Buttermilk‐Based Formulas Modulate Gut Microbiota in Mice with Antibiotic‐Induced Dysbiosis

“…[ 14 ] Moreover, the potential of whey proteins in the modulation of gut microbiota has also been studied. [ 11,25 ] These findings provide evidence of the individual effects of these milk bioactive components on microbiota, however, there are still gaps regarding the possible application of functional foods made from a combination of these components. Therefore, in this study we evaluated the potential of two formulas, containing a mixture of lactoferrin, MFGM, and whey or buttermilk, to reverse Clin‐induced effects on intestinal microbiota.…”

Donkey whey protein and peptides regulate gut microbiota community and physiological functions of D‐galactose‐induced aging mice

“…Therefore, appropriately increasing the diversity of intestinal flora can promote the body to improve antioxidant capacity and resist aging. But more needs to be done to support this mechanism (Ma et al, 2021 ).…”

Artigo de revisão: Propriedades biológicas das proteínas e peptídeos do soro do leite caprino

“…Além disso, efeitos a nível imunológico foram observados, tais como a estimulação de imunoglobulina G (IgG), menores concentrações de citocinas inflamatórias como as interleucinas -2 e 6 (IL-2 e IL 6) e o aumento do peso do timo (órgão relacionado a função imunológica) (Z. Ma et al, 2021).…”