Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier

García‐Rodríguez, Alba; Vila, Laura; Cortés, Constanza; Hernández, Alba; Marcos, Ricard

doi:10.1186/s12989-018-0269-x

Cited by 59 publications

(29 citation statements)

References 51 publications

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“…Given the previous information found in the literature, in this study we worked with a complex Caco-2/HT29 barrier model, trying to better mimic the interaction between graphene nanomaterials and the gastrointestinal tract. This model has recently shown to be useful to understand the role of the different shapes of TiO 2 NPs in their uptake and their consequent biological effects 17 . In addition, this model demonstrated that the cellular uptake of AgNPs showed a time-dependent and concentration-dependent increase 30 .…”

Section: Discussionmentioning

confidence: 99%

Interactions of graphene oxide and graphene nanoplatelets with the in vitro Caco-2/HT29 model of intestinal barrier

Domenech

Hernández

Demir

et al. 2020

Sci Rep

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Carbon-based nanomaterials are being increasingly used, demanding strong information to support their safety in terms of human health. As ingestion is one of the most important exposure routes in humans, we have determined their potential risk by using an in vitro model simulating the human intestinal barrier and evaluated the effects of both graphene oxide (GO) and graphene nanoplatelets (GNPs). A coculture of differentiated Caco-2/HT29 cells presenting inherent intestinal epithelium characteristics (i.e. mucus secretion, brush border, tight junctions, etc.) were treated with GO or GNPs for 24 h. Different endpoints such as viability, membrane integrity, NPs localization, cytokines secretion, and genotoxic damage were evaluated to have a wide view of their potentially harmful effects. No cytotoxic effects were observed in the cells that constitute the barrier model. In the same way, no adverse effects were detected neither in the integrity of the barrier (TEER) nor in its permeability (LY). Nevertheless, a different bio-adhesion and biodistribution behavior was observed for GO and GNPs by confocal microscopy analysis, with a more relevant uptake of GNPs. No oxidative damage induction was detected, either by the DCFH-DA assay or the FPG enzyme in the comet assay. Conversely, both GO and GNPs were able to induce DNA breaks, as observed in the comet assay. Finally, low levels of anti-inflammatory cytokines were detected, suggesting a weak anti-inflammatory response. Our results show the moderate/severe risk posed by GO/GNPs exposures, given the observed genotoxic effects, suggesting that more extensive genotoxic evaluations must be done to properly assess the genotoxic hazard of these nanomaterials. Carbon-based nanomaterials are being increasingly used, due to their growing relevance in many fields. Their large surface area and their excellent electric conductivity offer many physicochemical advantages that are exploited in many industrial fields, including biomedical applications 1. Nevertheless, as it occurs with other nanomaterials, its wide use supposes an increased hazard for human health 2. One of the most recently discovered carbon-based nanomaterials is graphene. Since its first description in 2004 3 , different modifications have been done in their single-atom-thick sheet of planar sp 2-bound carbons, creating numerous graphene-based nanomaterials. According to the EU Graphene Flagship project, these materials can be classified according to three different characteristics: (i) the number of graphene layers, (ii) the average lateral size, and (iii) the carbon/oxygen ratio 4,5. Although a large amount of literature associated with graphene-based nanomaterials has been generated, only a few of them focus on toxicity aspects 2,6. Hence, this paper focuses on graphene oxide (GO) and graphene nanoplatelets (GNPs) exposure risk, two graphene-based nanomaterials that have shown great potential for biomedical and polymeric composites applications 7,8. GO is a highly-oxidized form of graphene, containing different ...

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Section: Discussionmentioning

confidence: 99%

Interactions of graphene oxide and graphene nanoplatelets with the in vitro Caco-2/HT29 model of intestinal barrier

Domenech

Hernández

Demir

et al. 2020

Sci Rep

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“…A more recent study has found that hydrogen titanate (H 2 Ti 3 O 7 ) nanowires triggered generation of cellular debris in eight specific cell lines at a dose of 10 μg/ml, which was attributed to the increased formation of autophagosome‐like vacuoles escaping into the cytosol (Park, Shim, Lee, Kim, & Kim, 2013). García‐Rodríguez, Vila, Cortés, Hernández, and Marcos (2018) examined possible toxic effects of TiO 2 NPs exposing three distinctly shaped particles (i.e., nanowires [NWs], nanorods [NRs], and nanospheres [NSs]) to Caco‐2/HT29 cells, and they concluded that the greatest amount of damage to cells was caused by NWs. Lastly, Demir (2020), viability (toxicity), internalization (cellular uptake), ROS production, and genotoxicity (Comet assay) of TiO 2 NPs (NWs, NRs, and NSs) were the endpoints examined in hemocyte D. melanogaster larvae.…”

Section: Semiconductorsmentioning

confidence: 99%

A review on nanotoxicity and nanogenotoxicity of different shapes of nanomaterials

Demir

2020

J of Applied Toxicology

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Nanomaterials (NMs) generally display fascinating physical and chemical properties that are not always present in bulk materials; therefore, any modification to their size, shape, or coating tends to cause significant changes in their chemical/physical and biological characteristics. The dramatic increase in efforts to use NMs renders the risk assessment of their toxicity highly crucial due to the possible health perils of this relatively uncharted territory. The different sizes and shapes of the nanoparticles are known to have an impact on organisms and an important place in clinical applications. The shape of nanoparticles, namely, whether they are rods, wires, or spheres, is a particularly critical parameter to affect cell uptake and site-specific drug delivery, representing a significant factor in determining the potency and magnitude of the effect. This review, therefore, intends to offer a picture of research into the toxicity of different shapes (nanorods, nanowires, and nanospheres) of NMs to in vitro and in vivo models, presenting an in-depth analysis of health risks associated with exposure to such nanostructures and benefits achieved by using certain model organisms in genotoxicity testing. Nanotoxicity experiments use various models and tests, such as cell cultures, cores, shells, and coating materials. This review article also attempts to raise awareness about practical applications of NMs in different shapes in biology, to evaluate their potential genotoxicity, and to suggest approaches to explain underlying mechanisms of their toxicity and genotoxicity depending on nanoparticle shape.

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“…Specifically, TiO 2 NP disrupted the integrity and permeability of cell membrane and influenced the nutrient absorption of intestine in a shapedependent manner with the order: nanowires > nanorods > nanospheres. [64] In this case, it is worthy to note that TiO 2 NMs can downregulate the expression of alkaline phosphatase that is associated with the detoxification of lipopolysaccharide (LPS) and reduce the number of micro villi, thus resulted in decreased transport of iron, zinc, and fatty acids. [30] Similar with this study, CeO 2 nanorods with a long aspect ratio can blunt microvilli and interfere with the nutrient Figure 3.…”

Section: Nano-iec Interaction and Subsequent Biological Effectsmentioning

confidence: 99%

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

Cui

Bao

Wang

et al. 2020

Small

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201907665. Engineered nanomaterials (ENMs) are used in food additives, food packages, and therapeutic purposes owing to their useful properties, Therefore, human beings are orally exposed to exogenous nanomaterials frequently, which means the intestine is one of the primary targets of nanomaterials. Consequently, it is of great importance to understand the interaction between nanomaterials and the intestine. When nanomaterials enter into gut lumen, they inevitably interact with various components and thereby display different effects on the intestine based on their locations; these are known as location-oriented effects (LOE). The intestinal LOE confer a new biological-effect profile for nanomaterials, which is dependent on the involvement of the following biological processes: nanomucus interaction, nano-intestinal epithelial cells (IECs) interaction, nanoimmune interaction, and nano-microbiota interaction. A deep understanding ofNM-induced LOE will facilitate the design of safer NMs and the development of more efficient nanomedicine for intestine-related diseases. Herein, recent progress in this field is reviewed in order to better understand the LOE of nanomaterials. The distant effects of nanomaterials coupling with microbiota are also highlighted. Investigation of the interaction of nanomaterials with the intestine will stimulate other new research areas beyond intestinal nanotoxicity. www.advancedsciencenews.com

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Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier

Cited by 59 publications

References 51 publications

Interactions of graphene oxide and graphene nanoplatelets with the in vitro Caco-2/HT29 model of intestinal barrier

Interactions of graphene oxide and graphene nanoplatelets with the in vitro Caco-2/HT29 model of intestinal barrier

A review on nanotoxicity and nanogenotoxicity of different shapes of nanomaterials

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

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