Effects of dilute acid pretreatment conditions on enzymatic hydrolysis monomer and oligomer sugar yields for aspen, balsam, and switchgrass

Jensen, Jill R.; Morinelly, Juan E.; Gossen, Kelsey R.; Brodeur-Campbell, Michael J.; Shonnard, David R.

doi:10.1016/j.biortech.2009.11.038

Cited by 107 publications

(55 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Untreated corn stover, switch grass, and hatched wheat straw were ground, washed with deionized water, and dried in an oven at 100°C. For some assays, the corn stover was first pretreated using dilute sulfuric acid as described previously (40). Briefly, 90 ml of 0.8% sulfuric acid was incubated with 3 g ground corn stover.…”

Section: Methodsmentioning

confidence: 99%

Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass

Anderson

Miller

Fierobe

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

Lignocellulosic biomass is a promising feedstock to produce biofuels and other valuable biocommodities. A major obstacle to its commercialization is the high cost of degrading biomass into fermentable sugars, which is typically achieved using cellulolytic enzymes from Trichoderma reesei. Here, we explore the use of microbes to break down biomass. Bacillus subtilis was engineered to display a multicellulase-containing minicellulosome. The complex contains a miniscaffoldin protein that is covalently attached to the cell wall and three noncovalently associated cellulase enzymes derived from Clostridium cellulolyticum (Cel48F, Cel9E, and Cel5A). The minicellulosome spontaneously assembles, thus increasing the practicality of the cells. The recombinant bacteria are highly cellulolytic and grew in minimal medium containing industrially relevant forms of biomass as the primary nutrient source (corn stover, hatched straw, and switch grass). Notably, growth did not require dilute acid pretreatment of the biomass and the cells achieved densities approaching those of cells cultured with glucose. An analysis of the sugars released from acid-pretreated corn stover indicates that the cells have stable cellulolytic activity that enables them to break down 62.3% ؎ 2.6% of the biomass. When supplemented with beta-glucosidase, the cells liberated 21% and 33% of the total available glucose and xylose in the biomass, respectively. As the cells display only three types of enzymes, increasing the number of displayed enzymes should lead to even more potent cellulolytic microbes. This work has important implications for the efficient conversion of lignocellulose to value-added biocommodities.

show abstract

Section: Methodsmentioning

confidence: 99%

Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass

Anderson

Miller

Fierobe

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…These results might be occurred because of using juvenile lignocellulosic biomass which is less lignified. Compared to the dilute acid pretreatment, similarly, the maximum overall sugar yield was not augmented continuously, however log CS values (between 2.2 and 2.4) achieving the maximum overall sugar yield were 3 times higher than with organosolv pretreatment (Hsu et al, 2010;Jensen et al, 2010). These results probably related to complex and/or individual degradation aspects of lignocellulosic biomass major components, and further research is required to reveal fundamental characteristics of lignocellulosic biomass and design pretreatment process much economically.…”

Section: Overall Sugar Yieldmentioning

confidence: 92%

Organosolv Pretreatment of Slurry Composting and Biofiltration of Liquid Fertilizer-Treated Yellow Poplar for Sugar Production

Kim¹,

Gwak²,

Jang

et al. 2015

Journal of the Korean Wood Science and Technology

View full text Add to dashboard Cite

“…The main aim of these hydrolysis combinations is usually the production of hydrolysate containing fermentable sugars, which can be further processed into various biochemicals, such as bioethanol (Cara et al 2008). Acid hydrolysis has been utilized as a pretreatment method for numerous lignocellulosic feedstocks, such as agricultural wastes (Binod et al 2010;Romero et al 2010;Talebnia et al 2010;Rocha et al 2014;Singh et al 2014), but also for woody biomass (Söderström et al 2003;Jensen et al 2010;Wei et al 2012). The pretreatment time and temperature are strongly dependent on the concentration of the used acid and on the wanted outcomes.…”

Section: Acidic Pretreatmentsmentioning

confidence: 99%

Chemical Pretreatments of Wood Chips Prior to Alkaline Pulping - A Review of Pretreatment Alternatives, Chemical Aspects of the Resulting Liquors, and Pulping Outcomes

Lehto

Alén

2015

BioResources

View full text Add to dashboard Cite

The chemical industry is being forced to evaluate new strategies for more effective utilization of renewable feedstocks to diminish the use of fossil resources. In this literature review, the integration of both acidic and alkaline pretreatment phases of hardwood and softwood chips with chemical pulping is discussed. Depending on the pretreatment conditions, high-volume sulfur-free fractions with varying chemical compositions can be produced. In case of acidic pretreatments, the major products include carbohydrates (mono-, oligo-, and polysaccharides), whereas under alkaline (i.e., aqueous NaOH) pretreatment conditions, the sulfur-free fractions of aliphatic carboxylic acids, lignin, and extractives are primarily obtained. All these fractions are potentially interesting groups of compounds and can be used in a number of applications. Finally, the effects of pretreatments on pulping are also considered. Although it is believed that there are important advantages to be gained by integrating this type of renewable raw material-based production, in particular, with kraft pulping, sulfur-free pulping methods such as soda-AQ and oxygen/alkali delignification processes are also briefly discussed.

show abstract

Effects of dilute acid pretreatment conditions on enzymatic hydrolysis monomer and oligomer sugar yields for aspen, balsam, and switchgrass

Cited by 107 publications

References 22 publications

Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass

Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass

Organosolv Pretreatment of Slurry Composting and Biofiltration of Liquid Fertilizer-Treated Yellow Poplar for Sugar Production

Chemical Pretreatments of Wood Chips Prior to Alkaline Pulping - A Review of Pretreatment Alternatives, Chemical Aspects of the Resulting Liquors, and Pulping Outcomes

Contact Info

Product

Resources

About