Effects of dinoflagellate Gymnodinium catenatum on swimming behavior and expression of heat shock protein (hsp) genes in the brine shrimp Artemia franciscana

Han, Jeonghoon; Park, Yeun; Shin, Hyeon Ho; Shin, Ayoung; Kang, Hye-Min; Lee, Jihoon; Choi, Young-Ung; Lee, Kyun-Woo

doi:10.1016/j.hal.2021.102146

Cited by 6 publications

(5 citation statements)

References 51 publications

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“…Therefore, it was necessary to investigate the mechanisms involved through gene expression of HSPs, which actively participate in bioresilience processes in the genus Artemia. [11][12][13][14][15][60][61][62][63] Compared to the other treatments, an increase in Hsp26 and p26 gene expression was seen in cysts and nauplii treated with Isotherapic (R. raciborskii extract) 200cH. The protein p26 is a small HSP that is abundant in A. franciscana embryos during diapause.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the gene expression of specific HSPs has been reported as a critical element in controlling cyst hatching and embryo development of Artemia spp. [11][12][13][14][15] It was also investigated here.…”

Section: Hsp Gene Expression Involved In Artemia Franciscana Bioresil...mentioning

confidence: 99%

“…For gene expression analyses of specific mRNA (Hsp26, p26, Hsp40, Hsp90), [12][13][14][15] 2 μg of total RNA was used for cDNA synthesis with MMLV RNAse H minus first strand cDNA synthesis Kit (Nova Biotecnologia, Cotia, Brazil), according to the manufacturer's instructions. Quantitative polymerase chain reaction (qPCR) was carried out in a total of 10 μL, containing SYBR green qPCR Master Mix 2x (5 μL) (Nova Biotecnologia, Cotia, Brazil), specific primers (5 μM), and 60 ng of cDNA nuclease-free H 2 O in a QuantStudio 3 Real-Time PCR (Thermo Fisher Scientific, Waltham, Massachusetts, United States).…”

Section: Hsp Gene Expression Involved In Artemia Franciscana Bioresil...mentioning

confidence: 99%

“…In this way, the basal metabolism of the embryos remains reduced, and there is greater tolerance to any stressful factor from the external environment, such as toxins, temperature variations, dissection and others. Although the production of heat shock proteins (HSPs) is a crucial factor in this process, [11][12][13][14][15] the structure of the cyst is also quite peculiar, composed of a vitrified shell of non-glucose-reducing disaccharides, such as trehalose, which form bridges of hydrogen with phospholipids and macromolecules, giving the cysts excellent resistance. Trehalose also serves as an energy source for the embryo at diapause termination.…”

Section: Introductionmentioning

confidence: 99%

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Environmental Homeopathy: Homeopathic Potencies Regulate the Toxicity and Growth of Raphidiopsis raciborskii (cyanobacteria) and can be Tracked Physico-Chemically. Part 1: Biological Results

Mohammad,

Pinto,

Silva

et al. 2024

Homeopathy

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Introduction Cyanobacteria are microorganisms found in many parts of the world and several genera, such as Raphidiopsis raciborskii, are producers of cyanotoxins. Homeopathic potencies have been found to modulate toxicity in different biological models, and the present study endeavors to discover whether this might also be the case with cyanobacteria. Objectives Our objective was to investigate the possible effects of homeopathic potencies on the resilience of Artemia franciscana (brine shrimp) embryos to saxitoxin (STX; cyanotoxin) and on controlling the growth of R. raciborskii in vitro. Method A. franciscana cysts were cultivated in seawater in 96-well plates to evaluate the hatching rate and vitality, plus the gene expression of heat shock proteins (HSPs), after being challenged with R. raciborskii extract containing 2.5 µg/L of STX and treated with different homeopathic potencies. Untreated wells were used as controls (“base-line”). Potencies were chosen from a screening process based on seven selected homeopathic preparations according to the similitude of STX symptoms (Sulphur, Zincum metallicum, Nitric acidum, Plumbum metallicum, Mercurius solubilis, Phosphoric acidum, Isotherapic from R. raciborskii extract; all at 6cH, 30cH and 200cH). Cultures of R. raciborskii maintained in an artificial seawater medium were equally treated with screened homeopathic potencies selected from the same list but specifically for their growth control as a function of time. Results A 15% lower rate of hatching of A. franciscana cysts was observed after treatment with Nitric acidum 6cH in comparison with baseline (p = 0.05). A complete toxicity reversal was seen after treatment with Isotherapic 200cH, with a 23-fold increase of Hsp 26 gene expression (p = 0.023) and a 24-fold increase of p26 gene expression (p ≤ 0.001) in relation to baseline. Nitric acidum 200cH and Mercurius solubilis 30cH limited the exponential growth of cyanobacteria up to 95% and 85% respectively (p ≤ 0.003) in relation to baseline. Succussed water presented only a transitory 50% inhibition effect. Conclusion Isotherapic 200cH improved A. franciscana bioresilience to STX; Nitric acidum 200cH and Mercurius solubilis 30cH showed the optimal performance on limiting R. raciborskii growth. The results point to the potential of homeopathic potencies to mitigate environmental problems related to water quality.

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Section: Discussionmentioning

confidence: 99%

Section: Hsp Gene Expression Involved In Artemia Franciscana Bioresil...mentioning

confidence: 99%

Section: Hsp Gene Expression Involved In Artemia Franciscana Bioresil...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Environmental Homeopathy: Homeopathic Potencies Regulate the Toxicity and Growth of Raphidiopsis raciborskii (cyanobacteria) and can be Tracked Physico-Chemically. Part 1: Biological Results

Mohammad,

Pinto,

Silva

et al. 2024

Homeopathy

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“…The hsp 26 and p26 genes code for heat shock proteins, which can be considered bio-resilience markers for Artemia sp. [2][3][4][5][6][7][8][9][10] Additionally, Nitric acidum 200cH and Mercurius solubilis 30cH were the best agents limiting the exponential growth of R. raciborskii in artificial seawater medium (ASM)-1, 1 a rich medium considered ideal for cyanobacteria growth in laboratory conditions. 11 These results, taken together, highlight the potential of homeopathy in mitigating environmental problems related to water quality.…”

Section: Introductionmentioning

confidence: 99%

Environmental Homeopathy: Homeopathic Potencies Regulate the Growth and Toxicity of Raphidiopsis raciborskii (cyanobacteria) and Can be Tracked Physico-Chemically. Part 2: Physico-chemical Results

Mohammad,

Pinto,

Silva

et al. 2024

Homeopathy

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Introduction The control of cyanobacterial toxicity and growth by homeopathic potencies was described in Part 1 of this two-part report. Here, a parallel approach characterized the physico-chemical features of the potencies used and the liquid media treated with them, correlating these results with their respective biological effects. Objectives Our objective was to establish if physico-chemical parameters can track homeopathic potencies in seawater or artificial seawater medium (ASM)-1 and to discover whether these parameters correlate with previously described biological effects. Method Artemia franciscana (brine shrimp) cysts were cultivated in seawater challenged with Raphidiopsis raciborskii extract and treated with different homeopathic potencies chosen from a screening process. Cultures of R. raciborskii maintained in ASM-1 were also treated with previously screened homeopathic potencies, and their growth was monitored as a function of time. The physico-chemical properties of the treated media (seawater or ASM-1) were evaluated by their interaction with solvatochromic dyes and changes in pH, conductivity and temperature. Results Coumarin 7 was found to be a marker for Nitric acidum 6cH and Isotherapic (R. raciborskii extract) 200cH in seawater (analysis of variance [ANOVA], p = 0.0015). Nile red was found to be a marker for Nitric acidum 200cH and Mercurius solubilis 30cH in ASM-1 (ANOVA, p ≤ 0.001). An increase in pH of ASM-1 and endothermic effects were observed after these treatments (two-way ANOVA, p = 0.0001). Seawater and ASM-1 to which potencies had been added were also subjected to a constant unidirectional 2,400 Gauss static magnetic field and found to have enhanced effects on the solvatochromic dyes tested. Conclusion Homeopathic potencies were specifically traceable in aqueous media using solvatochromic dyes, especially when the samples were subjected to a magnetic field. Results from monitoring other physical parameters, such as pH and temperature, were less specific in relation to potency tracking. However, potency-induced endothermic effects might provide valuable thermodynamic data relating to the nature of potencies.

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Influence of visible light LEDs modulation techniques on photocatalytic degradation of ceftriaxone in a flat plate reactor

Morante,

Tammaro,

Albarano

et al. 2024

Chemical Engineering Journal

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Effects of dinoflagellate Gymnodinium catenatum on swimming behavior and expression of heat shock protein (hsp) genes in the brine shrimp Artemia franciscana

Cited by 6 publications

References 51 publications

Environmental Homeopathy: Homeopathic Potencies Regulate the Toxicity and Growth of Raphidiopsis raciborskii (cyanobacteria) and can be Tracked Physico-Chemically. Part 1: Biological Results

Environmental Homeopathy: Homeopathic Potencies Regulate the Toxicity and Growth of Raphidiopsis raciborskii (cyanobacteria) and can be Tracked Physico-Chemically. Part 1: Biological Results

Environmental Homeopathy: Homeopathic Potencies Regulate the Growth and Toxicity of Raphidiopsis raciborskii (cyanobacteria) and Can be Tracked Physico-Chemically. Part 2: Physico-chemical Results

Influence of visible light LEDs modulation techniques on photocatalytic degradation of ceftriaxone in a flat plate reactor

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