Effects of Dioxin Exposure on Thyroid Hormones of Populations Living in hot Spots of Dioxin Contamination in Vietnam

Khoa, Tran Van

doi:10.4172/2329-8847.1000141

Cited by 2 publications

(2 citation statements)

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“…Serum levels of dioxins (TCDD and other types) were analyzed by using a chemical-activated luciferase gene expression (DR-CALUX) bioassay that evaluates levels of TCDD/other forms of dioxins, as previously described (Khoa et al 2015). In brief, fat was extracted from 2 ml serum/subject using first 8 ml n-hexane (Sigma, St. Louis, MO) and then 2 ml 2-propanol (Merck, Darmstadt, Germany).…”

Section: Serum Dioxin Levelsmentioning

confidence: 99%

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Expression of aryl hydrocarbon receptor, inflammatory cytokines, and incidence of rheumatoid arthritis in Vietnamese dioxin-exposed people

Nguyen

Nakahama

Dang

et al. 2017

Journal of Immunotoxicology

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Many Vietnamese citizens have been and continue to be inadvertently exposed to dioxins and dioxin-like compounds deposited in the country during the Vietnam War. Dioxins may be involved in the pathogenesis of inflammatory diseases in part via by affecting expression of aryl hydrocarbon receptor (Ahr) and inflammatory cytokines in animal models. As the role of the Ahr in dioxin-exposed people is not well defined, a study was conducted to examine gene expression levels of Ahr, inflammatory cytokines, and the incidence of diseases in dioxin-exposed citizens who had/still resided near a heavily dioxin-contaminated area in Vietnam. Whole blood from citizens at/around Da Nang airbase and control individuals living in unsprayed areas was collected. Serum levels of dioxins were analyzed by using a dioxins-responsive chemical-activated luciferase gene expression bioassay. Gene expression of Ahr, interleukin (IL)-1β, TNFα, IL-6, and IL-22 in whole blood was examined by quantitative real-time PCR. The results showed levels of dioxins and expression of Ahr, IL-1β, TNFα, and IL-6 were up-regulated while IL-22 expression was down-regulated in dioxin-exposed people. Various disease incidences in the study subjects was also examined. Interestingly, the incidence of rheumatoid arthritis (RA) in these individuals was increased compared to the estimated prevalence of this disease in the general Vietnamese population. Analyses also showed that expression levels of Ahr correlated to those of IL-6 and IL-22 in the dioxin-exposed people. Taken together, dioxins might be involved in an up-regulated expression of Ahr that might possibly relate to changes in level of inflammatory cytokines and, ultimately, in the incidence of select diseases in residents of Vietnam who had/continue to live near a dioxins-contaminated site.

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Section: Serum Dioxin Levelsmentioning

confidence: 99%

“…After 24 h at 25 C, luciferase activity in each well was measured (luciferase assay system; Promega, Leiden, the Netherlands) in a Centro LB 960 Microplate Luminometer (Berthold Technologies, Bad Wildbad, Germany). Estimates of bioanalytic equivalents (BEQ)/g fat in each sample were then calculated as described in Khoa et al (2015).…”

Section: Serum Dioxin Levelsmentioning

confidence: 99%