Effects of dissolucytotic gold ions on recovering brain lesions

Danscher, Gorm

doi:10.1007/s00418-010-0681-2

Cited by 18 publications

(15 citation statements)

References 58 publications

(64 reference statements)

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“…Since gold in the Swarna bhasma is approximately 28–35 nm in size, it can reach the affected site on oral administration via intestinal absorption and possibly can release Au(I) ions in a sustained manner required for therapeutic action. [42] These results reinforce the application of Swarna bhasma as a therapeutic agent in clinical medicine from the safety point of view. These testing protocols may be adopted as a screening test for all bhasma preparations to meet the criteria that supports its use worldwide.…”

Section: Resultssupporting

confidence: 65%

Blood compatibility studies of Swarna bhasma (gold bhasma), an Ayurvedic drug

Paul

Sharma²

2011

Int J Ayurveda Res

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Swarna bhasma (gold bhasma) preparations are widely utilized as therapeutic agents. However, in vitro biological evaluations of bhasma preparations are needed along with the physicochemical characterization for present day standardization of metallic bhasma preparations to meet the criteria that supports its use. Therefore, an attempt has been made to evaluate the protein adsorption, blood compatibility and complement activation potential of two batches of Swarna bhasma preparation, along with its physicochemical characterization. The particle size, morphology, elemental analysis, and in vitro cytotoxicity were evaluated initially. Red blood cell hemolysis, aggregation studies with blood cells, protein adsorption, complement C3 adsorption, platelet activation and tight junction permeability in Caco-2 cell line were investigated. The Swarna bhasma preparations with a crystallite size of 28–35 nm did not induce any blood cell aggregation or protein adsorption. Activation potential of these preparations towards complement system or platelets was negligible. These particles were also non-cytotoxic. Swarna bhasma particles opened the tight junctions in Caco-2 cell experiments. The results suggest the application of Swarna bhasma preparations as a therapeutic agent in clinical medicine from the biological safety point of view.

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Section: Resultssupporting

confidence: 65%

Blood compatibility studies of Swarna bhasma (gold bhasma), an Ayurvedic drug

Paul

Sharma²

2011

Int J Ayurveda Res

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“…The results also allow a better understanding of the activity of gold implants: Danscher [29] and Danscher and Larsen [30] have shown that gold ions are liberated from gold implants, and that is now, on the basis of our studies, understandable as dissolution of active gold sites by free oxygen radicals which are typically formed in immune defense reactions.…”

Section: The Electrochemical Deposition Of Pt On Polycrystallinementioning

confidence: 82%

The effects of pretreatment of polycrystalline gold with OH• radicals on the electrochemical nucleation and growth of platinum

Sievers

Hasse

Scholz

2011

J Solid State Electrochem

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The nucleation and growth of platinum on polycrystalline gold was studied by chronoamperometry, cyclic voltammetry, and atomic force microscopy before and after treatment of the gold surface with hydroxyl (OH • ) radicals. Two different procedures of mechanical polishing of the gold surface ("coarse polish" and "fine polish") were applied before the treatment with OH • radicals. The nucleation and growth of Pt was much better reproducible on electrodes which underwent a "coarse polish". The treatment of the Au surface with OH • radicals decreased the number of active sites; however, the nucleation growth mode remained the same (3-D instantaneous). The spontaneous Pt deposition (no externally applied potential) on Au was unaffected by the treatment with OH • radicals. In situ atomic force microscopy experiments showed that the Pt starts to grow only on some of the Au grains, most probably on those which have active sites on their surface. This leads to a roughening of the electrode surface upon Pt deposition. Treatment with OH • radicals did only quantitatively diminish the amount of deposited Pt, but qualitatively the imaging of the Pt growth remained the same. Obviously, the OH • radicals lead to a knockout (decreasing number) of active sites for Pt nucleation, while the nature of the remaining active sites stays unaffected.

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“…However, gold ions, dissolucytotically released from metallic gold implants, have been shown to be anti-inflammatory [18,23-26]. Together with recent findings of decreased articular pain and inflammation in veterinary use [6,8], it is of interest to analyse the mode of action of metallic gold which can be applied to specific points of inflammation without systemic effects.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of macrophages: implications for an immunosuppressive effect of dissolucytotic gold ions

Seifert

Matussek

Sjögren

et al. 2012

Journal of Inflammation

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BackgroundGold salts has previously been used in the treatment of rheumatoid arthritis but have been replaced by biologicals such as TNF-α inhibitors. The mechanisms behind the anti-inflammatory effect of metallic gold ions are still unknown, however, recent data showed that charged gold atoms are released from pure metallic gold implants by macrophages via a dissolucytosis membrane, and that gold ions are taken up by local macrophages, mast cells and to some extent fibroblasts. These findings open the question of possible immunomodulatory effects of metallic gold and motivate efforts on a deeper understanding of the effect of metallic gold on key inflammatory cells as macrophages.MethodsHuman macrophage cells (cell line THP-1) were grown on gold foils and intracellular uptake was analysed by autometallography. The impact of phagocytised gold ions on viability of THP-1 cells was investigated by trypan blue staining and TUNEL assay. The global gene expression profile of THP-1 cells after incorporation of gold ions was studied using microarray analysis comprising approximately 20,000 genes. The gene expression data was confirmed by measurement of secreted proteins.ResultsAutometallography showed intracellular uptake of gold ions into THP-1 cells. No significant effect on viability of THP-1 cells was demonstrated. Our data revealed a unique gene expression signature of dissolucytotic THP-1 cells that had taken up gold ions. A large number of regulated genes were functionally related to immunomodulation. Gold ion uptake induced downregulation of genes involved in rheumatoid arthritis such as hepatocyte growth factor, tenascin-C, inhibitor of DNA binding 1 and 3 and matrix metalloproteinase 13.ConclusionThe data obtained in this study offer new insights into the mode of action of gold ions and suggest for the investigation of effects on other key cells and a possible future role of metallic gold as implants in rheumatoid arthritis or other inflammatory conditions.

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Effects of dissolucytotic gold ions on recovering brain lesions

Cited by 18 publications

References 58 publications

Blood compatibility studies of Swarna bhasma (gold bhasma), an Ayurvedic drug

Blood compatibility studies of Swarna bhasma (gold bhasma), an Ayurvedic drug

The effects of pretreatment of polycrystalline gold with OH• radicals on the electrochemical nucleation and growth of platinum

Gene expression profiling of macrophages: implications for an immunosuppressive effect of dissolucytotic gold ions

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