Effects of dividing on the growth and organogenesis of protocorm-like bodies in Doritaenopsis

Amaki, W.; Higuchi, Haruzo

doi:10.1016/0304-4238(89)90038-1

Cited by 10 publications

(11 citation statements)

References 3 publications

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“…It has been reported that the percentage of secondary PLB formation is higher in the basal parts than in the top parts of nPLBs (Amaki and Higuchi, 1989;Tanaka, 1987). In particular, when partially incised ePLBs had been subjected to light acclimation before culturing under high light conditions, a large number of secondary PLBs were formed from the basal half of the enlarged spheroidal part produced during the light acclimation period as well as the entire elongated rod-shaped part produced during dark culture (Fig.…”

Section: Discussionmentioning

confidence: 96%

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Development of a Highly Efficient and Simple Micropropagation System for Phalaenopsis Using Elongated Protocorm-like Bodies Induced by Skotomorphogenesis under Dark Conditions

Enoki

Takahara

2014

J. Japan. Soc. Hort. Sci.

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A highly efficient and simple micropropagation system for Phalaenopsis was developed using elongated protocorm-like bodies , of photosynthetic photon flux density respectively), PLB proliferation efficiency was higher under dark than high light and low light conditions. In addition, shoot formation percentage was lower under dark conditions (8.0%) than under low light (66.0%) and high light conditions (68.2%) and few PLBs developed shoots during culture under dark conditions. The Secondary ePLBs obtained after culturing under dark conditions were approximately twice as long as nPLBs. After acclimation under low light conditions for 2 weeks, the ePLBs were transferred to high light conditions after making a partial incision in their apical parts. Under high light conditions, a large number of secondary PLBs were obtained from ePLBs, 6 times as many as from nPLBs treated with the same partial incisions. The findings of this study showed that culturing PLBs in dark conditions suppresses shoot formation that might interfere with PLB proliferation, and that a large number of secondary PLBs could be obtained from these ePLBs compared with nPLBs after exposure to high light intensity.

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Section: Discussionmentioning

confidence: 96%

“…Each of the resulting PLBs can then easily be differentiated into a plantlet. The proliferation efficiency at this stage can be increased considerably by dividing PLB clusters into pieces (Amaki and Higuchi, 1989) and injuring PLB meristems (Nii et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Development of a Highly Efficient and Simple Micropropagation System for Phalaenopsis Using Elongated Protocorm-like Bodies Induced by Skotomorphogenesis under Dark Conditions

Enoki

Takahara

2014

J. Japan. Soc. Hort. Sci.

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“…Amaki and Higuchi (1989) reported that segments from the basal parts of PLBs can produce PLBs in Doritaenopsis in one long-term culture. Other reports revealed that PLB explants are useful for inducing callus and subsequent regeneration of PLBs in Phalaenopsis (Young et al, 2000), Dendrobium (Nayak et al, 2002), and Cymbidium (Nayak et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Several tissues have been used for in vitro culture, including flower-stalk nodes and internodes (Lin et al, 2000), cut ends of stems (Chen and Chang, 2000), protocorm-like body (PLB) sections (Young et al, 2000), leaves (Park et al, 2002), and shoot tips (Tokuhara and Mii, 2001). Amaki and Higuchi (1989) reported that long-term culture of segments from the basal part of PLBs produced a large number of secondary PLBs. However, the spectrum of PLB proliferation and the mode of their formation in repeated subcultures has not been investigated (Amaki and Higuchi, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…Amaki and Higuchi (1989) reported that long-term culture of segments from the basal part of PLBs produced a large number of secondary PLBs. However, the spectrum of PLB proliferation and the mode of their formation in repeated subcultures has not been investigated (Amaki and Higuchi, 1989). Induction of PLBs from leaf sections by direct somatic embryogenesis has been described for Phalaenopsis (Young et al, 2000;Park et al, 2002;Hsu and Chen, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids

et al. 2014

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ABSTRACT. Phalaenopsis orchids have been regenerated by inducing protocorm-like bodies (PLBs) from etiolated leaf sections. However, the physiological and molecular mechanisms of secondary PLB development and subsequent proliferation have not been explored. Bisectionally cutting primary PLBs resulted in more secondary PLBs at 5 weeks, suggesting an embryogenic stem cell property imposed by wounding of primary PLB tissues. The ethylene precursors ethephon and 1-aminocyclopropanecarboxylic acid and the ethylene perception inhibitor silver nitrate increased PLB formation, while aminoethoxyvinylglycine decreased PLB formation. Ethylene content in wounded PLB explants increased over culture time in media containing ethylene precursors or inhibitors. mRNA levels of PhACS2, PhACS3, and PhACO were increased by ethephon and decreased by ethylene inhibitors. Expression of genes in the ethylene signaling pathway was enhanced following ethylene-precursor treatment and was mitigated by ethylene inhibitors during PLB proliferation. Transcription of PhETR and PhEIN3, as well as PhERS, PhCTR, and PhGTP, was significantly increased 12 h after ethylene treatment. Ethylene and physical wounding stimulated secondary PLB formation in Phalaenopsis, probably through ethylene biosynthesis and signal transduction.

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2017

Micropropagation of Orchids

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Effects of dividing on the growth and organogenesis of protocorm-like bodies in Doritaenopsis

Cited by 10 publications

References 3 publications

Development of a Highly Efficient and Simple Micropropagation System for Phalaenopsis Using Elongated Protocorm-like Bodies Induced by Skotomorphogenesis under Dark Conditions

Development of a Highly Efficient and Simple Micropropagation System for Phalaenopsis Using Elongated Protocorm-like Bodies Induced by Skotomorphogenesis under Dark Conditions

Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids

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